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Q66431

- L_DUGBA

UniProt

Q66431 - L_DUGBA

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Protein

RNA-directed RNA polymerase L

Gene
L
Organism
Dugbe virus (isolate ArD44313) (DUGV)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Displays RNA-directed RNA polymerase and deubiquitinating activities. RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent innate immunity By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401For ubiquitin thioesterase activity By similarity
Active sitei151 – 1511For ubiquitin thioesterase activity By similarity

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  2. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  3. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  4. transcription, DNA-templated Source: InterPro
  5. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Protein family/group databases

MEROPSiC87.001.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 2 domains:
Ubiquitin thioesterase (EC:3.4.19.12)
Gene namesi
Name:L
OrganismiDugbe virus (isolate ArD44313) (DUGV)
Taxonomic identifieri766194 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeNairovirus
Virus hostiAmblyomma variegatum (Tropical bont tick) [TaxID: 34610]
Homo sapiens (Human) [TaxID: 9606]
Hyalomma marginatum rufipes [TaxID: 72862]
Hyalomma truncatum [TaxID: 72855]
Rhipicephalus [TaxID: 34630]
Rhipicephalus annulatus [TaxID: 34611]
Rhipicephalus decoloratus [TaxID: 60189]
Rhipicephalus geigyi [TaxID: 136141]
ProteomesiUP000000278: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 40364036RNA-directed RNA polymerase LPRO_0000222026Add
BLAST

Proteomic databases

PRIDEiQ66431.

Structurei

Secondary structure

1
4036
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 64
Beta strandi10 – 134
Beta strandi16 – 194
Helixi25 – 284
Beta strandi29 – 324
Beta strandi36 – 383
Helixi40 – 4910
Beta strandi50 – 523
Turni55 – 573
Helixi58 – 7215
Helixi73 – 753
Helixi77 – 804
Helixi86 – 938
Helixi102 – 11110
Beta strandi116 – 1216
Beta strandi125 – 13511
Beta strandi141 – 1466
Beta strandi148 – 1503
Beta strandi152 – 1576

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HXDX-ray2.85B/D2-169[»]
ProteinModelPortaliQ66431.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 158130OTUAdd
BLAST
Domaini2424 – 2639216RdRp catalyticAdd
BLAST

Domaini

The OTU domain is responsible for the deubiquitinating and deISGylation activities of Nsp2 By similarity.

Sequence similaritiesi

Contains 1 OTU domain.

Family and domain databases

InterProiIPR003323. OTU.
IPR007099. RNA-dir_pol_NSvirus.
IPR007322. RNA_pol_bunyavir.
[Graphical view]
PfamiPF04196. Bunya_RdRp. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66431-1 [UniParc]FASTAAdd to Basket

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MDFLDSLIWE RVVDEQYITN PTFCVSDYFE VIRQPGDGNC FYHSIAELFF     50
DVKTPSSFRK VKEHLQLAAE VYYDTEPEAV GTGISKDEYI KVAMKDNEWG 100
GSLEASMLSK HLQTTIILWV VNSTEQVTAA IKFGPGRVST ALNLMHVGRT 150
HFDALRIIEQ LENNQPQDRN RLDIADRIAA AEVYVRQSIE DNLQEDEFFD 200
YAREDEISED VSAPGGSREA TELKKKAILL NKTVKRGENI PIRVGRVLDC 250
LFSCKIAVSL DEGLLYLRPE TRESEATSIS LRQLGHKLLT RDRHIKMEYA 300
RSKLYVTRDL IDHLDIGGLL RSSFPGLGLE RYIQLLHSEL VLDLVTVVLA 350
VLLSTFLYGS NNKNKKQFIT NCLLNTKLSG KRVFKALSKL TGQMLYRTPK 400
RAVSIVSQEL YGKLMLKVKN NLEGMGPISM LALRNLNFDN MQLQDYLEML 450
SEMSKIDNSD VEYTHREISD LHTLVERLSK LQKSQDVNEL KLWFKEEVLT 500
KRSQRSVGNA FEFLINDYFK KKDIMKFVST SGKASSTGNI GNVLSYAHNL 550
YLSKESLRMT SEDVTQLLIE IRKLHKLQGD LSIEPVAIIC DKLEDQFRKL 600
FRELPEECSS ECQTLFNDIR NSPSHSVAWK HALRLKGTAY EGMFAKQYGW 650
SYISEDIKPS LTMIVQTLFP ESFEAFLDRT QLHPEFRDLT PDYALTQKIF 700
FPRNTIPRTE NRQLAIDVSL EGSVEAVPIV EKRMFPLPEV PIGEANSISR 750
VMNIFKEKRE ESMQKKLEHD RQAEANRLKS AGLSASKAEQ EVCNSAQDRK 800
EEKERTTEPA GKQQRTEDLV VIEGNQDEGD SDPQKKVDEK TVPGESKQHS 850
KSSGSSSTNQ MSQKVVDVPS VEDSSDQAPG DFPDYGYYFK RIVMDESGTV 900
LTEEAQLEKR QLLFIEVGYQ TDVDGKITTD YKKWKDILRL LELLNIKCSF 950
IACADCSSTP SNNWWISEDK VRLLKNSISH LFSKLTQNSP ADVTDIVVGS 1000
ISTQKVRSYL KSGTATKTPI SLKDVQETWS KMKDYIVNRP TGISLNKELV 1050
GALYQGLVEG AIISKEGTVN LIQMLKDKQE RITDEFERTK LKHEVNEDVK 1100
TSEKLLLGWL MEDLKGCRCM GCLTKIKELS ESMSVNQDRL EYLSTNCQTK 1150
SHCTECHPRS LEYRNISNVD NRVPSMQRVS HSRNEGFEDT NETLTELDRL 1200
VRLTLPGKTE KERRVKRNVE GLIRFMMQQS SLDCIKLPSG QIIAHRCSKK 1250
FKNSSEAEEK CNERFERLMK ELSEQKLKPY SDHVRKTITS SLKKTDKQAG 1300
SKCAVPRLWL ETLIRDLRVP TKDEEILLNI RTSMQSKTNF IRNNDKLIIR 1350
SNKEIADYLE TKRKNLLSEK ASDKIFSSDC ILFKEVIAEA LRRYYSTPYE 1400
GVPETIVKLI NFLCTFGWFQ EVVLYSKICE TFLRCCTEFS RSGIKLVKVR 1450
HCDTNLSIKL PSNKKENMLC CLYDKNMSLL KGPFFLNRRQ AILGSAYPYI 1500
LITLYIQVLQ QHRCLEVLNS VNDRVVGNIN TCTSNLLNTV KAELTLVNSG 1550
LFEKAYECRT EQCRLGGNFL NRSSRDHFIS TVSGLNVVYG ALIKDNLLAN 1600
SQPQNKQLQM LRFGMLCGLS RLSSALELGK KFSTSCRRIE DNIMRLYLQS 1650
TIYSANRDVS QNVQNWKMKD LCPDITIPCF SVYGLFVNSD RQRIFDIYNV 1700
HIYNKEMDNF DEGCITVLEE TAERHMLWEL DLLRSLEGDT RDVRAARLLL 1750
GCPNIRKATD KDGNRLMKKG ITDDWREEAG SDSSSISGRR SYASSGTRVK 1800
SMFGKYNSSQ KPFELKPGLE VVNDPLHDYK QAVQDSFCYS EYTPNTESVL 1850
KDCIHIIRTN PSHTMGSYEL IQAVTENARR KYPPENIERA RKDPKNWVSI 1900
SEVTETTSIV SQPRTHFMLK DCYKVLLGTE NKKIVKMLRG KLKKLGAMRT 1950
DIEIGKKDCL DLLTTVDGLS EEQCKNIVNG IFEPSKLSFY HWKDLLKKEL 2000
SEVLLTDDGN YIYCWLKTLS SMVKHSLKKD LRFMTGKNSL DIKPEMFTDE 2050
EYSALNIMKL ELLGEHTDGI QGKTDFLLSS WKKCALKPKE GQSILNVGLN 2100
SLAALHDELY DIRLQHLELT RIKKENPTVS FTKEEILVKR LEKGFLNKYK 2150
KEVMEAVNLI FYCCLTAPWC LHYKSLEAYL VRHPEILETE CIKENDIPLL 2200
DLTVTSLIRS LIDDIEGESS FNDSSDIKVR FAVKYLITLF TANGEPFSLS 2250
LNDGGLNDDL QLTTDEKLLY QTKKVFAKLG LSGNNYDFIW TLQMIANSNF 2300
NVCKRLTGRT TGERLPRSVR SKVIYEMVKL VGETGMAILQ QLAFSQALNY 2350
DHRFYAVLAP KAQLGGSRDL LVQETGTKVI HATTEMFSRN LLKTTSDDGL 2400
TNPHLKETRL NIGLDMLSTA RALDGKQVSD DSNLLNFFKT VCISGDNTKW 2450
GPIHCCSFFS GMMQQLLKDV QDWSSFYKLT FIKNLCRQIE IPAPSIRKIL 2500
NVLRFKLSDK GGVEKLSEEA IRSELINNLA EWEGNDTVKF LITTYISKGI 2550
MAMNSYNHMG QGIHHATSSL LTSMMAETFE ELAVDYMKKH FPGLTVNVDH 2600
AGSSDDYAKC IIVSGLVSKD MYKRYDGVFW RHMCRLKNFL AAVRRCCQMK 2650
DSAKTLVGDC FLEFYSEFMM GNRVTPAVIK FIFTGLINSS VTSPQSLVQA 2700
CHVSSQQGMY NSVPLVTNAA FTILRQQIFY NHVEDFIRRY GLITLGAVSP 2750
FGRLFLPRFS GLVSSSVALE DSETISKAAA EINSNDIFFN TSSLSNLDKL 2800
EQSPDSSGLD DDSVVSTTTV ESSDSKGSSS SFTFDLNRPL SETEVKFLKL 2850
LRELTSTTAC EMLQEKINTL YNDSREGPLD RHNILQNCRL SESCDWLLDG 2900
KKRGLLELSR RMSCLLNVLI AGYYRSFGSE GTEKQVKASW SRDDNRVIED 2950
PMIQLIPEKL RRELERLGLS RMEVDELMPA VGPDESLSQL VAKKLISLNV 3000
STEEYSAEVS RLKQTLTARN VLHGLAGGIK ELSLPIYTIF MKPYFFKDNV 3050
FLDLEDRWSS RHSTNYRDST GKMLTGKVVT KFTHWLDTFL SCVVSANRSQ 3100
EIKECSLFNP NLRCVNIMVK GNGIKELSYI RSHLSVLSVE FENLNLQFSD 3150
VNRQKLKIVE SRPPECELEA NKAVIIKSKL FSAVEHVRLS NNPAVVMGYL 3200
LEESSISEVK PTKVDFSNLL KHRFKLMQFF PSVFTLLRSL QSESKELEKL 3250
GEPVDMHQVS KYANHLTLLC RMIQQSKPSL TVFYMLKGSQ MNTEPTVSEL 3300
VSYGIKEGRF LKLPEIGLDA STYSVRYWKI LHCISAIGEL PLSSEDKTSL 3350
LISFLNWKVT SDCVDDCCPL EKYDKAIVSE FSGQVLINTL ASELSSVRKD 3400
QEREGLTDLI DYINSPSELL KKKPYLGTTC RFQCWGEGAK SGKFTYSSRT 3450
GEAIGIFVAG KLHIHLTSDS PGLLCEVERQ VLSWLGKRRT DVLTKEQHQF 3500
FLDFLPNLSE VVQKNRDGAI LGVTIDSTNV RMLKYVPPKR NTPVIKIKKQ 3550
ILTVKKQTTL DVESEPRIVW GHGQLSIVYD ECETETTYHE NLIKVKKLVD 3600
LASGTTDKLP TAIFSDTRIT LARVKFKTEL LLNSLCLLHC FLKHTSQDAI 3650
QEVESKCNVL ERYLRSGGVQ FRPMSESLDK KVTKLPLQCQ SDKDVDKEIN 3700
FCEDLTRVFS NENVPLSSWS EVQSYIEEVG FGNVLVHIEK NPTRSDLIWR 3750
FSIDSISGNF GPIKDIRTLV TYMSTETVPK FLLPFLLFEE QLKHLIAGCV 3800
ELRDALNSSG INDREIAIVA LFTCFYYQSD SVKRQGPVCS ISSFCSLIGD 3850
DLLPLDNRLQ ARVLPEQDNV KLHFKLNLTT DSALGKKDKA IQAKKIISRY 3900
LRLIFTEDDM DLKRLKSDAT KVKLSSEKEC EFLEFCLHSD LSYALNYRVL 3950
LEHLIDLEDR AKKTACVLIE EFILMLTGRL MISSTIDSDS KRTLEDDALC 4000
LEDLLDSDNE ASSSKSDNEE QIALQTGKFN FNWDSD 4036
Length:4,036
Mass (Da):459,392
Last modified:November 1, 1996 - v1
Checksum:iE2EDF0B4358E31BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15018 Genomic RNA. Translation: AAB18834.1.
RefSeqiNP_690576.1. NC_004159.1.

Genome annotation databases

GeneIDi956566.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15018 Genomic RNA. Translation: AAB18834.1 .
RefSeqi NP_690576.1. NC_004159.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HXD X-ray 2.85 B/D 2-169 [» ]
ProteinModelPortali Q66431.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C87.001.

Proteomic databases

PRIDEi Q66431.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956566.

Family and domain databases

InterProi IPR003323. OTU.
IPR007099. RNA-dir_pol_NSvirus.
IPR007322. RNA_pol_bunyavir.
[Graphical view ]
Pfami PF04196. Bunya_RdRp. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view ]
PROSITEi PS50802. OTU. 1 hit.
PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Large RNA segment of Dugbe nairovirus encodes the putative RNA polymerase."
    Marriott A.C., Nuttall P.A.
    J. Gen. Virol. 77:1775-1780(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiL_DUGBA
AccessioniPrimary (citable) accession number: Q66431
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi