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Q66431

- L_DUGBA

UniProt

Q66431 - L_DUGBA

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Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Dugbe virus (isolate ArD44313) (DUGV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Displays RNA-directed RNA polymerase and deubiquitinating activities. RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent innate immunity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401For ubiquitin thioesterase activityBy similarity
Active sitei151 – 1511For ubiquitin thioesterase activityBy similarity

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  2. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  3. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  4. transcription, DNA-templated Source: InterPro
  5. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Protein family/group databases

MEROPSiC87.001.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 2 domains:
Ubiquitin thioesterase (EC:3.4.19.12)
Gene namesi
Name:L
OrganismiDugbe virus (isolate ArD44313) (DUGV)
Taxonomic identifieri766194 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeNairovirus
Virus hostiAmblyomma variegatum (Tropical bont tick) [TaxID: 34610]
Homo sapiens (Human) [TaxID: 9606]
Hyalomma marginatum rufipes [TaxID: 72862]
Hyalomma truncatum [TaxID: 72855]
Rhipicephalus [TaxID: 34630]
Rhipicephalus annulatus [TaxID: 34611]
Rhipicephalus decoloratus [TaxID: 60189]
Rhipicephalus geigyi [TaxID: 136141]
ProteomesiUP000000278: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 40364036RNA-directed RNA polymerase LPRO_0000222026Add
BLAST

Proteomic databases

PRIDEiQ66431.

Structurei

Secondary structure

1
4036
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 64Combined sources
Beta strandi10 – 134Combined sources
Beta strandi16 – 194Combined sources
Helixi25 – 284Combined sources
Beta strandi29 – 324Combined sources
Beta strandi36 – 383Combined sources
Helixi40 – 4910Combined sources
Beta strandi50 – 523Combined sources
Turni55 – 573Combined sources
Helixi58 – 7215Combined sources
Helixi73 – 753Combined sources
Helixi77 – 804Combined sources
Helixi86 – 938Combined sources
Helixi102 – 11110Combined sources
Beta strandi116 – 1216Combined sources
Beta strandi125 – 13511Combined sources
Beta strandi141 – 1466Combined sources
Beta strandi148 – 1503Combined sources
Beta strandi152 – 1576Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HXDX-ray2.85B/D2-169[»]
ProteinModelPortaliQ66431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 158130OTUPROSITE-ProRule annotationAdd
BLAST
Domaini2424 – 2639216RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Domaini

The OTU domain is responsible for the deubiquitinating and deISGylation activities of Nsp2.By similarity

Sequence similaritiesi

Belongs to the nairovirus RNA polymerase family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR003323. OTU.
IPR007099. RNA-dir_pol_NSvirus.
IPR007322. RNA_pol_bunyavir.
[Graphical view]
PfamiPF04196. Bunya_RdRp. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66431-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MDFLDSLIWE RVVDEQYITN PTFCVSDYFE VIRQPGDGNC FYHSIAELFF
60 70 80 90 100
DVKTPSSFRK VKEHLQLAAE VYYDTEPEAV GTGISKDEYI KVAMKDNEWG
110 120 130 140 150
GSLEASMLSK HLQTTIILWV VNSTEQVTAA IKFGPGRVST ALNLMHVGRT
160 170 180 190 200
HFDALRIIEQ LENNQPQDRN RLDIADRIAA AEVYVRQSIE DNLQEDEFFD
210 220 230 240 250
YAREDEISED VSAPGGSREA TELKKKAILL NKTVKRGENI PIRVGRVLDC
260 270 280 290 300
LFSCKIAVSL DEGLLYLRPE TRESEATSIS LRQLGHKLLT RDRHIKMEYA
310 320 330 340 350
RSKLYVTRDL IDHLDIGGLL RSSFPGLGLE RYIQLLHSEL VLDLVTVVLA
360 370 380 390 400
VLLSTFLYGS NNKNKKQFIT NCLLNTKLSG KRVFKALSKL TGQMLYRTPK
410 420 430 440 450
RAVSIVSQEL YGKLMLKVKN NLEGMGPISM LALRNLNFDN MQLQDYLEML
460 470 480 490 500
SEMSKIDNSD VEYTHREISD LHTLVERLSK LQKSQDVNEL KLWFKEEVLT
510 520 530 540 550
KRSQRSVGNA FEFLINDYFK KKDIMKFVST SGKASSTGNI GNVLSYAHNL
560 570 580 590 600
YLSKESLRMT SEDVTQLLIE IRKLHKLQGD LSIEPVAIIC DKLEDQFRKL
610 620 630 640 650
FRELPEECSS ECQTLFNDIR NSPSHSVAWK HALRLKGTAY EGMFAKQYGW
660 670 680 690 700
SYISEDIKPS LTMIVQTLFP ESFEAFLDRT QLHPEFRDLT PDYALTQKIF
710 720 730 740 750
FPRNTIPRTE NRQLAIDVSL EGSVEAVPIV EKRMFPLPEV PIGEANSISR
760 770 780 790 800
VMNIFKEKRE ESMQKKLEHD RQAEANRLKS AGLSASKAEQ EVCNSAQDRK
810 820 830 840 850
EEKERTTEPA GKQQRTEDLV VIEGNQDEGD SDPQKKVDEK TVPGESKQHS
860 870 880 890 900
KSSGSSSTNQ MSQKVVDVPS VEDSSDQAPG DFPDYGYYFK RIVMDESGTV
910 920 930 940 950
LTEEAQLEKR QLLFIEVGYQ TDVDGKITTD YKKWKDILRL LELLNIKCSF
960 970 980 990 1000
IACADCSSTP SNNWWISEDK VRLLKNSISH LFSKLTQNSP ADVTDIVVGS
1010 1020 1030 1040 1050
ISTQKVRSYL KSGTATKTPI SLKDVQETWS KMKDYIVNRP TGISLNKELV
1060 1070 1080 1090 1100
GALYQGLVEG AIISKEGTVN LIQMLKDKQE RITDEFERTK LKHEVNEDVK
1110 1120 1130 1140 1150
TSEKLLLGWL MEDLKGCRCM GCLTKIKELS ESMSVNQDRL EYLSTNCQTK
1160 1170 1180 1190 1200
SHCTECHPRS LEYRNISNVD NRVPSMQRVS HSRNEGFEDT NETLTELDRL
1210 1220 1230 1240 1250
VRLTLPGKTE KERRVKRNVE GLIRFMMQQS SLDCIKLPSG QIIAHRCSKK
1260 1270 1280 1290 1300
FKNSSEAEEK CNERFERLMK ELSEQKLKPY SDHVRKTITS SLKKTDKQAG
1310 1320 1330 1340 1350
SKCAVPRLWL ETLIRDLRVP TKDEEILLNI RTSMQSKTNF IRNNDKLIIR
1360 1370 1380 1390 1400
SNKEIADYLE TKRKNLLSEK ASDKIFSSDC ILFKEVIAEA LRRYYSTPYE
1410 1420 1430 1440 1450
GVPETIVKLI NFLCTFGWFQ EVVLYSKICE TFLRCCTEFS RSGIKLVKVR
1460 1470 1480 1490 1500
HCDTNLSIKL PSNKKENMLC CLYDKNMSLL KGPFFLNRRQ AILGSAYPYI
1510 1520 1530 1540 1550
LITLYIQVLQ QHRCLEVLNS VNDRVVGNIN TCTSNLLNTV KAELTLVNSG
1560 1570 1580 1590 1600
LFEKAYECRT EQCRLGGNFL NRSSRDHFIS TVSGLNVVYG ALIKDNLLAN
1610 1620 1630 1640 1650
SQPQNKQLQM LRFGMLCGLS RLSSALELGK KFSTSCRRIE DNIMRLYLQS
1660 1670 1680 1690 1700
TIYSANRDVS QNVQNWKMKD LCPDITIPCF SVYGLFVNSD RQRIFDIYNV
1710 1720 1730 1740 1750
HIYNKEMDNF DEGCITVLEE TAERHMLWEL DLLRSLEGDT RDVRAARLLL
1760 1770 1780 1790 1800
GCPNIRKATD KDGNRLMKKG ITDDWREEAG SDSSSISGRR SYASSGTRVK
1810 1820 1830 1840 1850
SMFGKYNSSQ KPFELKPGLE VVNDPLHDYK QAVQDSFCYS EYTPNTESVL
1860 1870 1880 1890 1900
KDCIHIIRTN PSHTMGSYEL IQAVTENARR KYPPENIERA RKDPKNWVSI
1910 1920 1930 1940 1950
SEVTETTSIV SQPRTHFMLK DCYKVLLGTE NKKIVKMLRG KLKKLGAMRT
1960 1970 1980 1990 2000
DIEIGKKDCL DLLTTVDGLS EEQCKNIVNG IFEPSKLSFY HWKDLLKKEL
2010 2020 2030 2040 2050
SEVLLTDDGN YIYCWLKTLS SMVKHSLKKD LRFMTGKNSL DIKPEMFTDE
2060 2070 2080 2090 2100
EYSALNIMKL ELLGEHTDGI QGKTDFLLSS WKKCALKPKE GQSILNVGLN
2110 2120 2130 2140 2150
SLAALHDELY DIRLQHLELT RIKKENPTVS FTKEEILVKR LEKGFLNKYK
2160 2170 2180 2190 2200
KEVMEAVNLI FYCCLTAPWC LHYKSLEAYL VRHPEILETE CIKENDIPLL
2210 2220 2230 2240 2250
DLTVTSLIRS LIDDIEGESS FNDSSDIKVR FAVKYLITLF TANGEPFSLS
2260 2270 2280 2290 2300
LNDGGLNDDL QLTTDEKLLY QTKKVFAKLG LSGNNYDFIW TLQMIANSNF
2310 2320 2330 2340 2350
NVCKRLTGRT TGERLPRSVR SKVIYEMVKL VGETGMAILQ QLAFSQALNY
2360 2370 2380 2390 2400
DHRFYAVLAP KAQLGGSRDL LVQETGTKVI HATTEMFSRN LLKTTSDDGL
2410 2420 2430 2440 2450
TNPHLKETRL NIGLDMLSTA RALDGKQVSD DSNLLNFFKT VCISGDNTKW
2460 2470 2480 2490 2500
GPIHCCSFFS GMMQQLLKDV QDWSSFYKLT FIKNLCRQIE IPAPSIRKIL
2510 2520 2530 2540 2550
NVLRFKLSDK GGVEKLSEEA IRSELINNLA EWEGNDTVKF LITTYISKGI
2560 2570 2580 2590 2600
MAMNSYNHMG QGIHHATSSL LTSMMAETFE ELAVDYMKKH FPGLTVNVDH
2610 2620 2630 2640 2650
AGSSDDYAKC IIVSGLVSKD MYKRYDGVFW RHMCRLKNFL AAVRRCCQMK
2660 2670 2680 2690 2700
DSAKTLVGDC FLEFYSEFMM GNRVTPAVIK FIFTGLINSS VTSPQSLVQA
2710 2720 2730 2740 2750
CHVSSQQGMY NSVPLVTNAA FTILRQQIFY NHVEDFIRRY GLITLGAVSP
2760 2770 2780 2790 2800
FGRLFLPRFS GLVSSSVALE DSETISKAAA EINSNDIFFN TSSLSNLDKL
2810 2820 2830 2840 2850
EQSPDSSGLD DDSVVSTTTV ESSDSKGSSS SFTFDLNRPL SETEVKFLKL
2860 2870 2880 2890 2900
LRELTSTTAC EMLQEKINTL YNDSREGPLD RHNILQNCRL SESCDWLLDG
2910 2920 2930 2940 2950
KKRGLLELSR RMSCLLNVLI AGYYRSFGSE GTEKQVKASW SRDDNRVIED
2960 2970 2980 2990 3000
PMIQLIPEKL RRELERLGLS RMEVDELMPA VGPDESLSQL VAKKLISLNV
3010 3020 3030 3040 3050
STEEYSAEVS RLKQTLTARN VLHGLAGGIK ELSLPIYTIF MKPYFFKDNV
3060 3070 3080 3090 3100
FLDLEDRWSS RHSTNYRDST GKMLTGKVVT KFTHWLDTFL SCVVSANRSQ
3110 3120 3130 3140 3150
EIKECSLFNP NLRCVNIMVK GNGIKELSYI RSHLSVLSVE FENLNLQFSD
3160 3170 3180 3190 3200
VNRQKLKIVE SRPPECELEA NKAVIIKSKL FSAVEHVRLS NNPAVVMGYL
3210 3220 3230 3240 3250
LEESSISEVK PTKVDFSNLL KHRFKLMQFF PSVFTLLRSL QSESKELEKL
3260 3270 3280 3290 3300
GEPVDMHQVS KYANHLTLLC RMIQQSKPSL TVFYMLKGSQ MNTEPTVSEL
3310 3320 3330 3340 3350
VSYGIKEGRF LKLPEIGLDA STYSVRYWKI LHCISAIGEL PLSSEDKTSL
3360 3370 3380 3390 3400
LISFLNWKVT SDCVDDCCPL EKYDKAIVSE FSGQVLINTL ASELSSVRKD
3410 3420 3430 3440 3450
QEREGLTDLI DYINSPSELL KKKPYLGTTC RFQCWGEGAK SGKFTYSSRT
3460 3470 3480 3490 3500
GEAIGIFVAG KLHIHLTSDS PGLLCEVERQ VLSWLGKRRT DVLTKEQHQF
3510 3520 3530 3540 3550
FLDFLPNLSE VVQKNRDGAI LGVTIDSTNV RMLKYVPPKR NTPVIKIKKQ
3560 3570 3580 3590 3600
ILTVKKQTTL DVESEPRIVW GHGQLSIVYD ECETETTYHE NLIKVKKLVD
3610 3620 3630 3640 3650
LASGTTDKLP TAIFSDTRIT LARVKFKTEL LLNSLCLLHC FLKHTSQDAI
3660 3670 3680 3690 3700
QEVESKCNVL ERYLRSGGVQ FRPMSESLDK KVTKLPLQCQ SDKDVDKEIN
3710 3720 3730 3740 3750
FCEDLTRVFS NENVPLSSWS EVQSYIEEVG FGNVLVHIEK NPTRSDLIWR
3760 3770 3780 3790 3800
FSIDSISGNF GPIKDIRTLV TYMSTETVPK FLLPFLLFEE QLKHLIAGCV
3810 3820 3830 3840 3850
ELRDALNSSG INDREIAIVA LFTCFYYQSD SVKRQGPVCS ISSFCSLIGD
3860 3870 3880 3890 3900
DLLPLDNRLQ ARVLPEQDNV KLHFKLNLTT DSALGKKDKA IQAKKIISRY
3910 3920 3930 3940 3950
LRLIFTEDDM DLKRLKSDAT KVKLSSEKEC EFLEFCLHSD LSYALNYRVL
3960 3970 3980 3990 4000
LEHLIDLEDR AKKTACVLIE EFILMLTGRL MISSTIDSDS KRTLEDDALC
4010 4020 4030
LEDLLDSDNE ASSSKSDNEE QIALQTGKFN FNWDSD
Length:4,036
Mass (Da):459,392
Last modified:November 1, 1996 - v1
Checksum:iE2EDF0B4358E31BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15018 Genomic RNA. Translation: AAB18834.1.
RefSeqiNP_690576.1. NC_004159.1.

Genome annotation databases

GeneIDi956566.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15018 Genomic RNA. Translation: AAB18834.1 .
RefSeqi NP_690576.1. NC_004159.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HXD X-ray 2.85 B/D 2-169 [» ]
ProteinModelPortali Q66431.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C87.001.

Proteomic databases

PRIDEi Q66431.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956566.

Family and domain databases

InterProi IPR003323. OTU.
IPR007099. RNA-dir_pol_NSvirus.
IPR007322. RNA_pol_bunyavir.
[Graphical view ]
Pfami PF04196. Bunya_RdRp. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view ]
PROSITEi PS50802. OTU. 1 hit.
PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Large RNA segment of Dugbe nairovirus encodes the putative RNA polymerase."
    Marriott A.C., Nuttall P.A.
    J. Gen. Virol. 77:1775-1780(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiL_DUGBA
AccessioniPrimary (citable) accession number: Q66431
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3