Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q66431

- L_DUGBA

UniProt

Q66431 - L_DUGBA

Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Dugbe virus (isolate ArD44313) (DUGV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Displays RNA-directed RNA polymerase and deubiquitinating activities. RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent innate immunity By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei40 – 401For ubiquitin thioesterase activityBy similarity
    Active sitei151 – 1511For ubiquitin thioesterase activityBy similarity

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
    2. suppression by virus of host ISG15 activity Source: UniProtKB-KW
    3. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
    4. transcription, DNA-templated Source: InterPro
    5. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Viral immunoevasion, Viral RNA replication

    Keywords - Ligandi

    Nucleotide-binding

    Protein family/group databases

    MEROPSiC87.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase L
    Short name:
    Protein L
    Alternative name(s):
    Large structural protein
    Replicase
    Transcriptase
    Including the following 2 domains:
    Ubiquitin thioesterase (EC:3.4.19.12)
    Gene namesi
    Name:L
    OrganismiDugbe virus (isolate ArD44313) (DUGV)
    Taxonomic identifieri766194 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeNairovirus
    Virus hostiAmblyomma variegatum (Tropical bont tick) [TaxID: 34610]
    Homo sapiens (Human) [TaxID: 9606]
    Hyalomma marginatum rufipes [TaxID: 72862]
    Hyalomma truncatum [TaxID: 72855]
    Rhipicephalus [TaxID: 34630]
    Rhipicephalus annulatus [TaxID: 34611]
    Rhipicephalus decoloratus [TaxID: 60189]
    Rhipicephalus geigyi [TaxID: 136141]
    ProteomesiUP000000278: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 40364036RNA-directed RNA polymerase LPRO_0000222026Add
    BLAST

    Proteomic databases

    PRIDEiQ66431.

    Structurei

    Secondary structure

    1
    4036
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 64
    Beta strandi10 – 134
    Beta strandi16 – 194
    Helixi25 – 284
    Beta strandi29 – 324
    Beta strandi36 – 383
    Helixi40 – 4910
    Beta strandi50 – 523
    Turni55 – 573
    Helixi58 – 7215
    Helixi73 – 753
    Helixi77 – 804
    Helixi86 – 938
    Helixi102 – 11110
    Beta strandi116 – 1216
    Beta strandi125 – 13511
    Beta strandi141 – 1466
    Beta strandi148 – 1503
    Beta strandi152 – 1576

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HXDX-ray2.85B/D2-169[»]
    ProteinModelPortaliQ66431.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 158130OTUPROSITE-ProRule annotationAdd
    BLAST
    Domaini2424 – 2639216RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The OTU domain is responsible for the deubiquitinating and deISGylation activities of Nsp2.By similarity

    Sequence similaritiesi

    Belongs to the nairovirus RNA polymerase family.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR007099. RNA-dir_pol_NSvirus.
    IPR007322. RNA_pol_bunyavir.
    [Graphical view]
    PfamiPF04196. Bunya_RdRp. 1 hit.
    PF02338. OTU. 1 hit.
    [Graphical view]
    PROSITEiPS50802. OTU. 1 hit.
    PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q66431-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFLDSLIWE RVVDEQYITN PTFCVSDYFE VIRQPGDGNC FYHSIAELFF     50
    DVKTPSSFRK VKEHLQLAAE VYYDTEPEAV GTGISKDEYI KVAMKDNEWG 100
    GSLEASMLSK HLQTTIILWV VNSTEQVTAA IKFGPGRVST ALNLMHVGRT 150
    HFDALRIIEQ LENNQPQDRN RLDIADRIAA AEVYVRQSIE DNLQEDEFFD 200
    YAREDEISED VSAPGGSREA TELKKKAILL NKTVKRGENI PIRVGRVLDC 250
    LFSCKIAVSL DEGLLYLRPE TRESEATSIS LRQLGHKLLT RDRHIKMEYA 300
    RSKLYVTRDL IDHLDIGGLL RSSFPGLGLE RYIQLLHSEL VLDLVTVVLA 350
    VLLSTFLYGS NNKNKKQFIT NCLLNTKLSG KRVFKALSKL TGQMLYRTPK 400
    RAVSIVSQEL YGKLMLKVKN NLEGMGPISM LALRNLNFDN MQLQDYLEML 450
    SEMSKIDNSD VEYTHREISD LHTLVERLSK LQKSQDVNEL KLWFKEEVLT 500
    KRSQRSVGNA FEFLINDYFK KKDIMKFVST SGKASSTGNI GNVLSYAHNL 550
    YLSKESLRMT SEDVTQLLIE IRKLHKLQGD LSIEPVAIIC DKLEDQFRKL 600
    FRELPEECSS ECQTLFNDIR NSPSHSVAWK HALRLKGTAY EGMFAKQYGW 650
    SYISEDIKPS LTMIVQTLFP ESFEAFLDRT QLHPEFRDLT PDYALTQKIF 700
    FPRNTIPRTE NRQLAIDVSL EGSVEAVPIV EKRMFPLPEV PIGEANSISR 750
    VMNIFKEKRE ESMQKKLEHD RQAEANRLKS AGLSASKAEQ EVCNSAQDRK 800
    EEKERTTEPA GKQQRTEDLV VIEGNQDEGD SDPQKKVDEK TVPGESKQHS 850
    KSSGSSSTNQ MSQKVVDVPS VEDSSDQAPG DFPDYGYYFK RIVMDESGTV 900
    LTEEAQLEKR QLLFIEVGYQ TDVDGKITTD YKKWKDILRL LELLNIKCSF 950
    IACADCSSTP SNNWWISEDK VRLLKNSISH LFSKLTQNSP ADVTDIVVGS 1000
    ISTQKVRSYL KSGTATKTPI SLKDVQETWS KMKDYIVNRP TGISLNKELV 1050
    GALYQGLVEG AIISKEGTVN LIQMLKDKQE RITDEFERTK LKHEVNEDVK 1100
    TSEKLLLGWL MEDLKGCRCM GCLTKIKELS ESMSVNQDRL EYLSTNCQTK 1150
    SHCTECHPRS LEYRNISNVD NRVPSMQRVS HSRNEGFEDT NETLTELDRL 1200
    VRLTLPGKTE KERRVKRNVE GLIRFMMQQS SLDCIKLPSG QIIAHRCSKK 1250
    FKNSSEAEEK CNERFERLMK ELSEQKLKPY SDHVRKTITS SLKKTDKQAG 1300
    SKCAVPRLWL ETLIRDLRVP TKDEEILLNI RTSMQSKTNF IRNNDKLIIR 1350
    SNKEIADYLE TKRKNLLSEK ASDKIFSSDC ILFKEVIAEA LRRYYSTPYE 1400
    GVPETIVKLI NFLCTFGWFQ EVVLYSKICE TFLRCCTEFS RSGIKLVKVR 1450
    HCDTNLSIKL PSNKKENMLC CLYDKNMSLL KGPFFLNRRQ AILGSAYPYI 1500
    LITLYIQVLQ QHRCLEVLNS VNDRVVGNIN TCTSNLLNTV KAELTLVNSG 1550
    LFEKAYECRT EQCRLGGNFL NRSSRDHFIS TVSGLNVVYG ALIKDNLLAN 1600
    SQPQNKQLQM LRFGMLCGLS RLSSALELGK KFSTSCRRIE DNIMRLYLQS 1650
    TIYSANRDVS QNVQNWKMKD LCPDITIPCF SVYGLFVNSD RQRIFDIYNV 1700
    HIYNKEMDNF DEGCITVLEE TAERHMLWEL DLLRSLEGDT RDVRAARLLL 1750
    GCPNIRKATD KDGNRLMKKG ITDDWREEAG SDSSSISGRR SYASSGTRVK 1800
    SMFGKYNSSQ KPFELKPGLE VVNDPLHDYK QAVQDSFCYS EYTPNTESVL 1850
    KDCIHIIRTN PSHTMGSYEL IQAVTENARR KYPPENIERA RKDPKNWVSI 1900
    SEVTETTSIV SQPRTHFMLK DCYKVLLGTE NKKIVKMLRG KLKKLGAMRT 1950
    DIEIGKKDCL DLLTTVDGLS EEQCKNIVNG IFEPSKLSFY HWKDLLKKEL 2000
    SEVLLTDDGN YIYCWLKTLS SMVKHSLKKD LRFMTGKNSL DIKPEMFTDE 2050
    EYSALNIMKL ELLGEHTDGI QGKTDFLLSS WKKCALKPKE GQSILNVGLN 2100
    SLAALHDELY DIRLQHLELT RIKKENPTVS FTKEEILVKR LEKGFLNKYK 2150
    KEVMEAVNLI FYCCLTAPWC LHYKSLEAYL VRHPEILETE CIKENDIPLL 2200
    DLTVTSLIRS LIDDIEGESS FNDSSDIKVR FAVKYLITLF TANGEPFSLS 2250
    LNDGGLNDDL QLTTDEKLLY QTKKVFAKLG LSGNNYDFIW TLQMIANSNF 2300
    NVCKRLTGRT TGERLPRSVR SKVIYEMVKL VGETGMAILQ QLAFSQALNY 2350
    DHRFYAVLAP KAQLGGSRDL LVQETGTKVI HATTEMFSRN LLKTTSDDGL 2400
    TNPHLKETRL NIGLDMLSTA RALDGKQVSD DSNLLNFFKT VCISGDNTKW 2450
    GPIHCCSFFS GMMQQLLKDV QDWSSFYKLT FIKNLCRQIE IPAPSIRKIL 2500
    NVLRFKLSDK GGVEKLSEEA IRSELINNLA EWEGNDTVKF LITTYISKGI 2550
    MAMNSYNHMG QGIHHATSSL LTSMMAETFE ELAVDYMKKH FPGLTVNVDH 2600
    AGSSDDYAKC IIVSGLVSKD MYKRYDGVFW RHMCRLKNFL AAVRRCCQMK 2650
    DSAKTLVGDC FLEFYSEFMM GNRVTPAVIK FIFTGLINSS VTSPQSLVQA 2700
    CHVSSQQGMY NSVPLVTNAA FTILRQQIFY NHVEDFIRRY GLITLGAVSP 2750
    FGRLFLPRFS GLVSSSVALE DSETISKAAA EINSNDIFFN TSSLSNLDKL 2800
    EQSPDSSGLD DDSVVSTTTV ESSDSKGSSS SFTFDLNRPL SETEVKFLKL 2850
    LRELTSTTAC EMLQEKINTL YNDSREGPLD RHNILQNCRL SESCDWLLDG 2900
    KKRGLLELSR RMSCLLNVLI AGYYRSFGSE GTEKQVKASW SRDDNRVIED 2950
    PMIQLIPEKL RRELERLGLS RMEVDELMPA VGPDESLSQL VAKKLISLNV 3000
    STEEYSAEVS RLKQTLTARN VLHGLAGGIK ELSLPIYTIF MKPYFFKDNV 3050
    FLDLEDRWSS RHSTNYRDST GKMLTGKVVT KFTHWLDTFL SCVVSANRSQ 3100
    EIKECSLFNP NLRCVNIMVK GNGIKELSYI RSHLSVLSVE FENLNLQFSD 3150
    VNRQKLKIVE SRPPECELEA NKAVIIKSKL FSAVEHVRLS NNPAVVMGYL 3200
    LEESSISEVK PTKVDFSNLL KHRFKLMQFF PSVFTLLRSL QSESKELEKL 3250
    GEPVDMHQVS KYANHLTLLC RMIQQSKPSL TVFYMLKGSQ MNTEPTVSEL 3300
    VSYGIKEGRF LKLPEIGLDA STYSVRYWKI LHCISAIGEL PLSSEDKTSL 3350
    LISFLNWKVT SDCVDDCCPL EKYDKAIVSE FSGQVLINTL ASELSSVRKD 3400
    QEREGLTDLI DYINSPSELL KKKPYLGTTC RFQCWGEGAK SGKFTYSSRT 3450
    GEAIGIFVAG KLHIHLTSDS PGLLCEVERQ VLSWLGKRRT DVLTKEQHQF 3500
    FLDFLPNLSE VVQKNRDGAI LGVTIDSTNV RMLKYVPPKR NTPVIKIKKQ 3550
    ILTVKKQTTL DVESEPRIVW GHGQLSIVYD ECETETTYHE NLIKVKKLVD 3600
    LASGTTDKLP TAIFSDTRIT LARVKFKTEL LLNSLCLLHC FLKHTSQDAI 3650
    QEVESKCNVL ERYLRSGGVQ FRPMSESLDK KVTKLPLQCQ SDKDVDKEIN 3700
    FCEDLTRVFS NENVPLSSWS EVQSYIEEVG FGNVLVHIEK NPTRSDLIWR 3750
    FSIDSISGNF GPIKDIRTLV TYMSTETVPK FLLPFLLFEE QLKHLIAGCV 3800
    ELRDALNSSG INDREIAIVA LFTCFYYQSD SVKRQGPVCS ISSFCSLIGD 3850
    DLLPLDNRLQ ARVLPEQDNV KLHFKLNLTT DSALGKKDKA IQAKKIISRY 3900
    LRLIFTEDDM DLKRLKSDAT KVKLSSEKEC EFLEFCLHSD LSYALNYRVL 3950
    LEHLIDLEDR AKKTACVLIE EFILMLTGRL MISSTIDSDS KRTLEDDALC 4000
    LEDLLDSDNE ASSSKSDNEE QIALQTGKFN FNWDSD 4036
    Length:4,036
    Mass (Da):459,392
    Last modified:November 1, 1996 - v1
    Checksum:iE2EDF0B4358E31BD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15018 Genomic RNA. Translation: AAB18834.1.
    RefSeqiNP_690576.1. NC_004159.1.

    Genome annotation databases

    GeneIDi956566.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15018 Genomic RNA. Translation: AAB18834.1 .
    RefSeqi NP_690576.1. NC_004159.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HXD X-ray 2.85 B/D 2-169 [» ]
    ProteinModelPortali Q66431.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C87.001.

    Proteomic databases

    PRIDEi Q66431.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956566.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR007099. RNA-dir_pol_NSvirus.
    IPR007322. RNA_pol_bunyavir.
    [Graphical view ]
    Pfami PF04196. Bunya_RdRp. 1 hit.
    PF02338. OTU. 1 hit.
    [Graphical view ]
    PROSITEi PS50802. OTU. 1 hit.
    PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Large RNA segment of Dugbe nairovirus encodes the putative RNA polymerase."
      Marriott A.C., Nuttall P.A.
      J. Gen. Virol. 77:1775-1780(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiL_DUGBA
    AccessioniPrimary (citable) accession number: Q66431
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3