RNA-directed RNA polymerase L - Dugbe virus (isolate ArD44313) (DUGV)

Sequence or UniProt identifier

>sp|Q66431|L_DUGBA RNA-directed RNA polymerase L OS=Dugbe virus (isolate ArD44313) GN=L PE=1 SV=1 MDFLDSLIWERVVDEQYITNPTFCVSDYFEVIRQPGDGNCFYHSIAELFFDVKTPSSFRK VKEHLQLAAEVYYDTEPEAVGTGISKDEYIKVAMKDNEWGGSLEASMLSKHLQTTIILWV VNSTEQVTAAIKFGPGRVSTALNLMHVGRTHFDALRIIEQLENNQPQDRNRLDIADRIAA AEVYVRQSIEDNLQEDEFFDYAREDEISEDVSAPGGSREATELKKKAILLNKTVKRGENI PIRVGRVLDCLFSCKIAVSLDEGLLYLRPETRESEATSISLRQLGHKLLTRDRHIKMEYA RSKLYVTRDLIDHLDIGGLLRSSFPGLGLERYIQLLHSELVLDLVTVVLAVLLSTFLYGS NNKNKKQFITNCLLNTKLSGKRVFKALSKLTGQMLYRTPKRAVSIVSQELYGKLMLKVKN NLEGMGPISMLALRNLNFDNMQLQDYLEMLSEMSKIDNSDVEYTHREISDLHTLVERLSK LQKSQDVNELKLWFKEEVLTKRSQRSVGNAFEFLINDYFKKKDIMKFVSTSGKASSTGNI GNVLSYAHNLYLSKESLRMTSEDVTQLLIEIRKLHKLQGDLSIEPVAIICDKLEDQFRKL FRELPEECSSECQTLFNDIRNSPSHSVAWKHALRLKGTAYEGMFAKQYGWSYISEDIKPS LTMIVQTLFPESFEAFLDRTQLHPEFRDLTPDYALTQKIFFPRNTIPRTENRQLAIDVSL EGSVEAVPIVEKRMFPLPEVPIGEANSISRVMNIFKEKREESMQKKLEHDRQAEANRLKS AGLSASKAEQEVCNSAQDRKEEKERTTEPAGKQQRTEDLVVIEGNQDEGDSDPQKKVDEK TVPGESKQHSKSSGSSSTNQMSQKVVDVPSVEDSSDQAPGDFPDYGYYFKRIVMDESGTV LTEEAQLEKRQLLFIEVGYQTDVDGKITTDYKKWKDILRLLELLNIKCSFIACADCSSTP SNNWWISEDKVRLLKNSISHLFSKLTQNSPADVTDIVVGSISTQKVRSYLKSGTATKTPI SLKDVQETWSKMKDYIVNRPTGISLNKELVGALYQGLVEGAIISKEGTVNLIQMLKDKQE RITDEFERTKLKHEVNEDVKTSEKLLLGWLMEDLKGCRCMGCLTKIKELSESMSVNQDRL EYLSTNCQTKSHCTECHPRSLEYRNISNVDNRVPSMQRVSHSRNEGFEDTNETLTELDRL VRLTLPGKTEKERRVKRNVEGLIRFMMQQSSLDCIKLPSGQIIAHRCSKKFKNSSEAEEK CNERFERLMKELSEQKLKPYSDHVRKTITSSLKKTDKQAGSKCAVPRLWLETLIRDLRVP TKDEEILLNIRTSMQSKTNFIRNNDKLIIRSNKEIADYLETKRKNLLSEKASDKIFSSDC ILFKEVIAEALRRYYSTPYEGVPETIVKLINFLCTFGWFQEVVLYSKICETFLRCCTEFS RSGIKLVKVRHCDTNLSIKLPSNKKENMLCCLYDKNMSLLKGPFFLNRRQAILGSAYPYI LITLYIQVLQQHRCLEVLNSVNDRVVGNINTCTSNLLNTVKAELTLVNSGLFEKAYECRT EQCRLGGNFLNRSSRDHFISTVSGLNVVYGALIKDNLLANSQPQNKQLQMLRFGMLCGLS RLSSALELGKKFSTSCRRIEDNIMRLYLQSTIYSANRDVSQNVQNWKMKDLCPDITIPCF SVYGLFVNSDRQRIFDIYNVHIYNKEMDNFDEGCITVLEETAERHMLWELDLLRSLEGDT RDVRAARLLLGCPNIRKATDKDGNRLMKKGITDDWREEAGSDSSSISGRRSYASSGTRVK SMFGKYNSSQKPFELKPGLEVVNDPLHDYKQAVQDSFCYSEYTPNTESVLKDCIHIIRTN PSHTMGSYELIQAVTENARRKYPPENIERARKDPKNWVSISEVTETTSIVSQPRTHFMLK DCYKVLLGTENKKIVKMLRGKLKKLGAMRTDIEIGKKDCLDLLTTVDGLSEEQCKNIVNG IFEPSKLSFYHWKDLLKKELSEVLLTDDGNYIYCWLKTLSSMVKHSLKKDLRFMTGKNSL DIKPEMFTDEEYSALNIMKLELLGEHTDGIQGKTDFLLSSWKKCALKPKEGQSILNVGLN SLAALHDELYDIRLQHLELTRIKKENPTVSFTKEEILVKRLEKGFLNKYKKEVMEAVNLI FYCCLTAPWCLHYKSLEAYLVRHPEILETECIKENDIPLLDLTVTSLIRSLIDDIEGESS FNDSSDIKVRFAVKYLITLFTANGEPFSLSLNDGGLNDDLQLTTDEKLLYQTKKVFAKLG LSGNNYDFIWTLQMIANSNFNVCKRLTGRTTGERLPRSVRSKVIYEMVKLVGETGMAILQ QLAFSQALNYDHRFYAVLAPKAQLGGSRDLLVQETGTKVIHATTEMFSRNLLKTTSDDGL TNPHLKETRLNIGLDMLSTARALDGKQVSDDSNLLNFFKTVCISGDNTKWGPIHCCSFFS GMMQQLLKDVQDWSSFYKLTFIKNLCRQIEIPAPSIRKILNVLRFKLSDKGGVEKLSEEA IRSELINNLAEWEGNDTVKFLITTYISKGIMAMNSYNHMGQGIHHATSSLLTSMMAETFE ELAVDYMKKHFPGLTVNVDHAGSSDDYAKCIIVSGLVSKDMYKRYDGVFWRHMCRLKNFL AAVRRCCQMKDSAKTLVGDCFLEFYSEFMMGNRVTPAVIKFIFTGLINSSVTSPQSLVQA CHVSSQQGMYNSVPLVTNAAFTILRQQIFYNHVEDFIRRYGLITLGAVSPFGRLFLPRFS GLVSSSVALEDSETISKAAAEINSNDIFFNTSSLSNLDKLEQSPDSSGLDDDSVVSTTTV ESSDSKGSSSSFTFDLNRPLSETEVKFLKLLRELTSTTACEMLQEKINTLYNDSREGPLD RHNILQNCRLSESCDWLLDGKKRGLLELSRRMSCLLNVLIAGYYRSFGSEGTEKQVKASW SRDDNRVIEDPMIQLIPEKLRRELERLGLSRMEVDELMPAVGPDESLSQLVAKKLISLNV STEEYSAEVSRLKQTLTARNVLHGLAGGIKELSLPIYTIFMKPYFFKDNVFLDLEDRWSS RHSTNYRDSTGKMLTGKVVTKFTHWLDTFLSCVVSANRSQEIKECSLFNPNLRCVNIMVK GNGIKELSYIRSHLSVLSVEFENLNLQFSDVNRQKLKIVESRPPECELEANKAVIIKSKL FSAVEHVRLSNNPAVVMGYLLEESSISEVKPTKVDFSNLLKHRFKLMQFFPSVFTLLRSL QSESKELEKLGEPVDMHQVSKYANHLTLLCRMIQQSKPSLTVFYMLKGSQMNTEPTVSEL VSYGIKEGRFLKLPEIGLDASTYSVRYWKILHCISAIGELPLSSEDKTSLLISFLNWKVT SDCVDDCCPLEKYDKAIVSEFSGQVLINTLASELSSVRKDQEREGLTDLIDYINSPSELL KKKPYLGTTCRFQCWGEGAKSGKFTYSSRTGEAIGIFVAGKLHIHLTSDSPGLLCEVERQ VLSWLGKRRTDVLTKEQHQFFLDFLPNLSEVVQKNRDGAILGVTIDSTNVRMLKYVPPKR NTPVIKIKKQILTVKKQTTLDVESEPRIVWGHGQLSIVYDECETETTYHENLIKVKKLVD LASGTTDKLPTAIFSDTRITLARVKFKTELLLNSLCLLHCFLKHTSQDAIQEVESKCNVL ERYLRSGGVQFRPMSESLDKKVTKLPLQCQSDKDVDKEINFCEDLTRVFSNENVPLSSWS EVQSYIEEVGFGNVLVHIEKNPTRSDLIWRFSIDSISGNFGPIKDIRTLVTYMSTETVPK FLLPFLLFEEQLKHLIAGCVELRDALNSSGINDREIAIVALFTCFYYQSDSVKRQGPVCS ISSFCSLIGDDLLPLDNRLQARVLPEQDNVKLHFKLNLTTDSALGKKDKAIQAKKIISRY LRLIFTEDDMDLKRLKSDATKVKLSSEKECEFLEFCLHSDLSYALNYRVLLEHLIDLEDR AKKTACVLIEEFILMLTGRLMISSTIDSDSKRTLEDDALCLEDLLDSDNEASSSKSDNEE QIALQTGKFNFNWDSD

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Sequence length	4036 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Function	Displays RNA-directed RNA polymerase and deubiquitinating activities. RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent innate immunity By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Domain	The OTU domain is responsible for the deubiquitinating and deISGylation activities of Nsp2 By similarity.
Sequence similarities	Belongs to the nairovirus RNA polymerase family. Contains 1 OTU domain. Contains 1 RdRp catalytic domain.

Keywords
Biological process	Host-virus interaction Inhibition of host ISG15 by virus Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Modulation of host ubiquitin pathway by viral deubiquitinase Modulation of host ubiquitin pathway by virus Viral RNA replication Viral immunoevasion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	modulation by virus of host protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host ISG15 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Family and domain databases

Entry information

Relevant documents

[1]	"Large RNA segment of Dugbe nairovirus encodes the putative RNA polymerase." Marriott A.C., Nuttall P.A. J. Gen. Virol. 77:1775-1780(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
+	Additional computationally mapped references.