Reviewed, UniProtKB/Swiss-Prot Q662E4 (SYA_BORGA)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BG0223

Organism

Borrelia garinii [Complete proteome] [HAMAP]

Taxonomic identifier

29519 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm. HAMAP MF_00036
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075073

Sequences

	Sequence		Length	Mass (Da)
	Q662E4-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: C0ACE947C796332D	FASTA	594	67,664
	10 20 30 40 50 60 MTLDKLRKKY IDFFKSKKHF EIMGKSLVPE NDPTVLFNTA GMQPLIPYLL GEVHPSGDML 70 80 90 100 110 120 VNVQKCLRTG DIDEVGDLSH LTFFEMLGNW SLGAYFKEYS VKCSFEFLTS SDYLNIPKDS 130 140 150 160 170 180 LYVSVFEGDQ KIPCDTETAK VWESLGIPKD RIYYLSKAHN FWGPVGSKGP CGPDTEIYVD 190 200 210 220 230 240 TGKIKCSLDC NITCSCGKYF EIWNNVFMQY NKDENGNYIE LDRKCVDTGM GLERTIAFLQ 250 260 270 280 290 300 GKSSVYDTDA FIPIIKRIEV ISGKIYGQKE DDDRCIRIIS DHVKAACFIL ADSSVVSPSN 310 320 330 340 350 360 LGQGYVLRRL IRRSIRYAKK LGIKSHFLAD LVDSIETIYG SFYNELTEKK DFIKKELSTE 370 380 390 400 410 420 EEKFFKTLFQ GEQEFIKITR NLPSKTIPGD IAFKLYDTYG FPYEVTEELA FEYGFNIDKV 430 440 450 460 470 480 GFDEYFKKHQ KTSKKGGDKV FKGGLADYTY ETTRLHTATH LLHKALQLVL GDHVRQKGSN 490 500 510 520 530 540 ITAERLRFDF VHSEKMTDDE IKKVEEIVNL QIKNSLSVKK SIMELSEAQK KGAMALFGEK 550 560 570 580 590 YDNLVSVYEI DGFSLEVCGG PHVENTNELG TFKIQKEQSS SSGIRRIKAI LIDK

References

[1]

"Comparative analysis of the Borrelia garinii genome." Gloeckner G., Lehmann R., Romualdi A., Pradella S., Schulte-Spechtel U., Schilhabel M., Wilske B., Suehnel J., Platzer M. Nucleic Acids Res. 32:6038-6046(2004) [PubMed: 15547252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PBi.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000013 Genomic DNA. Translation: AAU07077.1.
RefSeq	YP_072669.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2957270.
GenomeReviews	Gene locus BG0223 in contig CP000013_GR.
KEGG	bga:BG0223.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q662E4.
OMA	DIDEVGD.
Enzyme and pathway databases
BioCyc	BGAR290434:BG0223-MON.
BRENDA	6.1.1.7. 294970.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BORGA

Accession

Primary (citable) accession number: Q662E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)