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Q662B4 (SYL_BORGA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BG0254
OrganismBorrelia garinii (strain PBi) [Complete proteome] [HAMAP]
Taxonomic identifier290434 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group

Protein attributes

Sequence length840 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 840840Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151980

Regions

Motif44 – 5512"HIGH" region HAMAP-Rule MF_00049
Motif617 – 6215"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q662B4 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 20A9FBE916589685

FASTA84098,168
        10         20         30         40         50         60 
MSKYEFIKIE KKWQEFWDNN KTYKVKEDPN IPKEKRLYIL DMFPYPSANG LHVGHPEGYT 

        70         80         90        100        110        120 
ATDIFGRYKL LNGFHVLHPI GFDSFGLPAE NYAIQTGTHP QKSTEENINK FKKQIKALGF 

       130        140        150        160        170        180 
AYDWDREIRT HDENYYKWTQ WIFLELYKKG LAYAKEMPVW YCPELGTVLA NEEIIQTPDG 

       190        200        210        220        230        240 
PKSERGFHNV EKKYLRQWVL KITKYAERLL NDLEELEWPE SVKEMQRNWI GKSTGVEIDF 

       250        260        270        280        290        300 
EIEGHNDKIK VFTTRPDTIF GITYLVIAPE SKLIEKITKN NFKRNVLKYV KHEELKSDLK 

       310        320        330        340        350        360 
RTSLEKDKSG VFTGSYAFHP ITNEKIPIWI GSYVLGTYGS GAVMGVPAHD ERDFQFAKKY 

       370        380        390        400        410        420 
KLKILPVISK SGKNEILEKA FIDDGISINS PNEFNNLKNS EVKDKVIEWL IKNKKGKEKV 

       430        440        450        460        470        480 
TYKLRDWIFS RQRYWGEPIP ILFDKLGNAV PLEKNDLPLK LPKTANYKPS RTGESPLSRI 

       490        500        510        520        530        540 
KDWVNLKDTG FTRETNTMPQ WAGSCWYYLR YLDPKNPKEF ASKKKIEYWM PVDLYIGGAE 

       550        560        570        580        590        600 
HTVLHLLYSR FWHKVLYDLG YVNTKEPFKK LINQGIITAF SYQKENGILI PNDQVIEKNS 

       610        620        630        640        650        660 
KFFDKRDNKE VIQVIAKMSK SLKNVINPDD IIKEFGADSI RIYEMFMGPL TDSKPWNTKG 

       670        680        690        700        710        720 
IIGVFRFLNK IWNLREKELS KDNPPKEIIS QLHKAIKKVT EDTEKLSFNT AISAMMIFVN 

       730        740        750        760        770        780 
ELIKYEKNYL NIFKPFIIIL SPYAPHLAEE LWEYIGETPS LFKNSKWPEF DENLIIKDAK 

       790        800        810        820        830        840 
EIVLQINGKM KDKILLSKET DEEELKEIAM GNSKIKANLL NKKIVKIIAI KNKLVNIVIK 

« Hide

References

[1]"Comparative analysis of the Borrelia garinii genome."
Gloeckner G., Lehmann R., Romualdi A., Pradella S., Schulte-Spechtel U., Schilhabel M., Wilske B., Suehnel J., Platzer M.
Nucleic Acids Res. 32:6038-6046(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PBi.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000013 Genomic DNA. Translation: AAU07107.1.
RefSeqYP_072699.1. NC_006156.1.

3D structure databases

ProteinModelPortalQ662B4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290434.BG0254.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU07107; AAU07107; BG0254.
GeneID2957256.
KEGGbga:BG0254.
PATRIC20566065. VBIBorGar102262_0358.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycBGAR290434:BG0254-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BORGA
AccessionPrimary (citable) accession number: Q662B4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries