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Protein

Replicase large subunit

Gene
N/A
Organism
Youcai mosaic virus (YoMV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The replicase large subunit is an RNA-dependent RNA polymerase active in viral RNA replication.By similarity
The replicase small subunit is a methyltransferase active in RNA capping and an RNA helicase. It also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May mediate silencing suppression through either inhibition of HEN1-mediated siRNA or siRNA demethylation (By similarity).By similarity

Miscellaneous

Readthrough of the terminator codon UAG occurs between codons for Gln-1103 and Gln-1105.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi823 – 830ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Nucleotidyltransferase, RNA-directed RNA polymerase, Suppressor of RNA silencing, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase large subunit (EC:2.7.7.48)
Alternative name(s):
182 kDa protein
Cleaved into the following chain:
Alternative name(s):
125 kDa protein
Methyltransferase/RNA helicase
Short name:
MT/HEL
OrganismiYoucai mosaic virus (YoMV)
Taxonomic identifieri228578 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageVirgaviridaeTobamovirus
Virus hostiBrassica napus (Rape) [TaxID: 3708]
Proteomesi
  • UP000008609 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000411741 – 1597Replicase large subunitAdd BLAST1597
ChainiPRO_00000411751 – 1103Replicase small subunitAdd BLAST1103

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 280Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST209
Domaini791 – 950(+)RNA virus helicase ATP-bindingAdd BLAST160
Domaini951 – 1103(+)RNA virus helicase C-terminalAdd BLAST153
Domaini1365 – 1478RdRp catalyticPROSITE-ProRule annotationAdd BLAST114

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG09000048.

Family and domain databases

InterProiView protein in InterPro
IPR027351. (+)RNA_virus_helicase_core_dom.
IPR002588. Alphavirus-like_MT_dom.
IPR027417. P-loop_NTPase.
IPR007094. RNA-dir_pol_PSvirus.
IPR001788. Tymovirus_RNA-dep_RNA_pol.
PfamiView protein in Pfam
PF00978. RdRP_2. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF01660. Vmethyltransf. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51743. ALPHAVIRUS_MT. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQFQQTVNM QTLQAAAGRN SLVNDLASRR VYDNAVEELN ARSRRPKVHF
60 70 80 90 100
SKSVSTEQTL LASNAYPEFE ISFTHTQQAV HSLAGGLRTL ELEYLMMQVP
110 120 130 140 150
FGSLTYDIGG NFAAHLFKGR DYVHCCMPNL DVRDIARHEG HKEAIFSYLS
160 170 180 190 200
RLDRQRRPVP EYQRAAFHNY AENPHFVHCD RPFQQCELST VNRWDTYAIA
210 220 230 240 250
LHSIYDIPAD EFGAALLRKN VKICYAAFHF HENMLLDCDS VTLEDIGATF
260 270 280 290 300
QRAGDKLNFF FHNESTLNYT HSFSNIIKYV CKTFFPASQR YVYHKEFLVT
310 320 330 340 350
RVNTWYCKFT RVDTFTLFRG VYKTSVDSEE FYKAMDDAWE YKKTLAMLNS
360 370 380 390 400
ERTIFKDSAA INFWFPKVRD MVIIPLFDAS ITTGRMSRRE VLVNKDFVYT
410 420 430 440 450
VLNHIKTYQA KALTYANVLS FVESIRSRVI INGVTARSEW DTDKAILGPL
460 470 480 490 500
AMTFFLVTKL SHVQDEIVLK KFQKFDATAK ELIWSSLCDA LKGVIPSVKE
510 520 530 540 550
TLARGGFVKL AEESLEIKIP ELYCTFTDRL VLEYKRTEEF QSCDLSKPLE
560 570 580 590 600
ESEKYYNALS ELSVLENLGS FDLDAFKELC QKKNVDPDVA AKVVVAIMNS
610 620 630 640 650
ELTLPFKKPT EEEVAEALSG EVVQDEGLRL SNKAPFPCVS NLKEGLVPAC
660 670 680 690 700
GLCPNGANFD RVDMDISEFH LKSVDAVKKG AMMSAVYTGK IKVQQMKNYV
710 720 730 740 750
DYLSASLSAT VSNLCKVLRD VHGVDPESQE KSGVWDVRRG RWLLKPNAKC
760 770 780 790 800
HAWGVAEDAN HKLVIVLLNW DEGKPVCDET WFRLAVSSDS LVYSDMGKLK
810 820 830 840 850
TLTSCCRDGE PPEPTAKLVL VDGVPGCGKT KEILEKVNFS EDLVLVPGKE
860 870 880 890 900
ASKMIIRRAN QAGITRADKD NVRTVDSFLM HPPKRVFKRL FIDEGLMLHT
910 920 930 940 950
GCVNFLMLLS HCDVAYVYVD TQQIPFICRV ANFPYPAHFA KLVVDEKEDR
960 970 980 990 1000
RVTLRCPADV TYFLNQKYDG SVLCTSSVER SVSAEVVRGK GALNPITLPL
1010 1020 1030 1040 1050
EGKILTFTQA DKFELLDKGY KDVNTVHEVQ GETYEKTAIV RLTATPLEII
1060 1070 1080 1090 1100
SRASPHVLVA LTRHTTRCKY YTVVLDPMVN VISELGKLSN FLLEMYKVES
1110 1120 1130 1140 1150
GTQXQLQIDT VFKGTNLFVP TPKSGDWRDM QFYYDTLLPG NSTILNEFDA
1160 1170 1180 1190 1200
VTMNLRDISL NVKDCRIDFS KSVQVPKERP VFMKPKLRTA AEMPRTAGLL
1210 1220 1230 1240 1250
ENLVAMIKRN MNAPDLTGTI DIEDTASLVV EKFWDAYVVK EFSGTDGMAM
1260 1270 1280 1290 1300
TRESFSRWLS KQESSTVGQL ADFNFVDLPA VDEYKHMIKS QPKQKLDLSI
1310 1320 1330 1340 1350
QDEYPALQTI VYHSKKINAI FGPMFSELTR MLLETIDTSK FLFYTRKTPT
1360 1370 1380 1390 1400
QIEEFFSDLD SSQAMEILEL DISKYDKSQN EFHCAVEYKI WEKLGIDDWL
1410 1420 1430 1440 1450
AEVWRQGHRK TTLKDYTAGI KTCLWYQRKS GDVTTFIGNT IIIAACLSSM
1460 1470 1480 1490 1500
IPMDKVIKAA FCGDDSLIYI PKGLDLPDIQ AGANLTWNFE AKLFRKKYGY
1510 1520 1530 1540 1550
FCGRYVIHHD RGAIVYYDPL KLISKLGCKH IRDEVHLEEL RRSLCDVTSN
1560 1570 1580 1590
LNNCAYFSQL DEAVAEVHKT AVGGAFVYCS IIKYLSDKRL FKDLFFV
Length:1,597
Mass (Da):181,624
Last modified:December 1, 2000 - v2
Checksum:i7256A908BD3308F2
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti919V → G. 1
Natural varianti1286H → Y. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30944 mRNA. Translation: AAB60599.2.
PIRiS65053.
RefSeqiNP_740757.2. NC_004422.1.

Genome annotation databases

GeneIDi955946.
KEGGivg:955946.

Keywords - Coding sequence diversityi

RNA suppression of termination

Similar proteinsi

Entry informationi

Entry nameiRDRP_YOMV
AccessioniPrimary (citable) accession number: Q66220
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 22, 2017
This is version 82 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families