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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Bovine coronavirus (strain G95) (BCoV) (BCV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system (By similarity).By similarity

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401NucleophileBy similarity
Active sitei326 – 3261Charge relay systemBy similarity
Active sitei329 – 3291Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2b
OrganismiBovine coronavirus (strain G95) (BCoV) (BCV)
Taxonomic identifieri230235 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 392374Virion surfaceSequence AnalysisAdd
BLAST
Transmembranei393 – 41321HelicalSequence AnalysisAdd
BLAST
Topological domaini414 – 42411IntravirionSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Chaini19 – 424406Hemagglutinin-esterasePRO_0000037135Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 65By similarity
Glycosylationi54 – 541N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi89 – 891N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi113 ↔ 162By similarity
Glycosylationi153 – 1531N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi197 ↔ 276By similarity
Disulfide bondi205 ↔ 249By similarity
Glycosylationi236 – 2361N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi301 – 3011N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi307 ↔ 312By similarity
Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi347 ↔ 371By similarity
Glycosylationi358 – 3581N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

N-glycosylated in the RER.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliQ66165.
SMRiQ66165. Positions 19-376.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 127121Esterase domain first partBy similarityAdd
BLAST
Regioni128 – 266139Receptor bindingBy similarityAdd
BLAST
Regioni267 – 379113Esterase domain second partBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66165-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLLPRFVLV SCIIGSLGFE NPPTNVVSHL NGDWFLFGDS RSDCNHVVNT
60 70 80 90 100
NPRNYSYMDL NPALCDSGKI SSKAGNSIFR SFHFTDFYNY TGEGQQIIFY
110 120 130 140 150
EGVNFTPYHA FKCTTSGSND IWMQNKGLFY TQVYKNMAVY RSLTFVNVPY
160 170 180 190 200
VYNGSAQSTA LCKSGSLVLN NPAYIAREAN FGDYYYKVEA DFYLSGCDEY
210 220 230 240 250
IVPLCIFNGK FLSNTKYYDD SQYYFNKDTG VIYGLNSTET ITTGFDFNCH
260 270 280 290 300
YLVLPSGNYL AISNELLLTV PTKAICLNKR KDFTPVQVVD SRWNNARQSD
310 320 330 340 350
NMTAVACQPP YCYFRNSTTN YVGVYDINHG DAGFTSILSG LLYDSPCFSQ
360 370 380 390 400
QGVFRYNNVS SVWPLYPYGR CPTAADINTP DVPICVYDPL PLILLGILLG
410 420
VAVIIIVVLL LYFMVDNGTR LHDA
Length:424
Mass (Da):47,702
Last modified:June 16, 2003 - v2
Checksum:iAA39CBAB56C8B8A4
GO

Sequence cautioni

The sequence AAA73152.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80842 mRNA. Translation: AAA73152.1. Different initiation.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80842 mRNA. Translation: AAA73152.1. Different initiation.

3D structure databases

ProteinModelPortaliQ66165.
SMRiQ66165. Positions 19-376.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The hemagglutinin/esterase gene of human coronavirus strain OC43: phylogenetic relationships to bovine and murine coronaviruses and influenza C virus."
    Zhang X.M., Kousoulas K.G., Storz J.
    Virology 186:318-323(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiHEMA_CVBG9
AccessioniPrimary (citable) accession number: Q66165
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: June 24, 2015
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.