Reviewed, UniProtKB/Swiss-Prot Q66145 (ORF2A_CMVMB)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase 2A
      Short name=protein 2A
    EC=2.7.7.48

Gene names

Name:	RNA2
ORF Names:	ORF2a

Organism

Cucumber mosaic virus (strain MB-8) (CMV)

Taxonomic identifier

117122 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Bromoviridae › Cucumovirus

Virus host

Spinacia oleracea (Spinach) [TaxID: 3562]
Cucumis sativus (Cucumber) [TaxID: 3659]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]

Protein attributes

Sequence length	857 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-directed RNA polymerase.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Miscellaneous	The virus has a tripartite genome (RNA1, RNA2 and RNA3) and an encapsidated subgenomic RNA (RNA4) from which the coat protein is expressed. Virions are icosahedral, not enveloped, with 32 capsomers per nucleocapsid.
Sequence similarities	Belongs to the cucumoviruses/bromoviruses 2A family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 857	857	RNA-directed RNA polymerase 2A		PRO_0000083278
Regions
	Domain	511 – 624	114	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q66145-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 658EC9F705C463D6	FASTA	857	96,671
	10 20 30 40 50 60 MAFPAPAFSL ANLLNGSYGV DTPEDVERLR SEQREEAAAA CRNYRPLSAV DVSESVTEDA 70 80 90 100 110 120 HSLQTPDGAP AEAVSDEFVT YGAEDYLEKS DDELLVAFET MVKPMRIGQL WCPAFNKCSF 130 140 150 160 170 180 ISSIAMARAL LLAPRTSHRT MKCFEDLVAA IYTKSDFYYS EECEADDVQM DISSRDVPGY 190 200 210 220 230 240 SFEPWSRTSG FEPPPICEAC DMIMYQCPCF DFNALKKSCA ERTFADDYVI EGLDGVVDNA 250 260 270 280 290 300 TLLSNLGPFL VPVKCQYEKC PTPPIAIPPN LNRATDRVDI NLVQSICDST LPTHSNYDDS 310 320 330 340 350 360 FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLN TGSGHKRVGT TKEVLTAIKK 370 380 390 400 410 420 RNADVPELGD SVNLSRLSKA VAERFFISYI NGNSLASSNF VNVVSNFHDY MEKWKSSGLS 430 440 450 460 470 480 YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPIPAT ITYHKKSITS QFSPLFTALF 490 500 510 520 530 540 ERFQRCLRER IILPVGKISS LEMAGFDVKN KYCLEIDLSK FDKSQGEFHL LIQEHILNGL 550 560 570 580 590 600 GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAF TYFGNTIVTM AEFAWCYDTD 610 620 630 640 650 660 QFEKLLFSGD DSLGFSLLPP VGDSSKFTTL YNMEAKVMEP SVPYICSKFL LSDEFGNTFS 670 680 690 700 710 720 VPDPLREVQR LGTKKIPYSD NDEFLFAHFM SFVDRLKFLD RMSQSCIDQL SIFFELKYKK 730 740 750 760 770 780 SGEEAALMLG AFKKYTANFQ SYKELYYSDR HQCELINSFC STEFRVERVN SNKQRKKYGI 790 800 810 820 830 840 ERRCDDKRRT PTGSYGGGEE AETKVSQTKS TGTRSQKSQR ESAFESQTVP LPTVLSSGWS 850 GTDRVVPPCE RGGVTRA

References

[1]	Karasawa A., Ito A., Okada I., Hase S., Ehara Y. Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D86613 Genomic RNA. Translation: BAA13141.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001788. RNA-dep_RNA_pol_vir-typ. IPR007094. RNA_pol_PSvir. [Graphical view]
Pfam	PF00978. RdRP_2. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ORF2A_CMVMB

Accession

Primary (citable) accession number: Q66145

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)