ID   ORF2A_CMVIX             Reviewed;         858 AA.
AC   Q66117;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 40.
DE   RecName: Full=RNA-directed RNA polymerase 2A;
DE            Short=protein 2A;
DE            EC=2.7.7.48;
GN   Name=RNA2; ORFNames=ORF2a;
OS   Cucumber mosaic virus (strain Ixora) (CMV).
OC   Viruses; ssRNA positive-strand viruses, no DNA stage; Bromoviridae;
OC   Cucumovirus.
OX   NCBI_TaxID=117114;
OH   NCBI_TaxID=3562; Spinacia oleracea (Spinach).
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   MEDLINE=96005047; PubMed=7561763;
RA   McGarvey P.B., Tousignant M., Geletka L., Cellini F., Kaper J.M.;
RT   "The complete sequence of a cucumber mosaic virus from Ixora that is
RT   deficient in the replication of satellite RNAs.";
RL   J. Gen. Virol. 76:2257-2270(1995).
CC   -!- FUNCTION: RNA-directed RNA polymerase.
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- MISCELLANEOUS: The virus has a tripartite genome (RNA1, RNA2 and
CC       RNA3) and an encapsidated subgenomic RNA (RNA4) from which the
CC       coat protein is expressed. Virions are icosahedral, not enveloped,
CC       with 32 capsomers per nucleocapsid.
CC   -!- SIMILARITY: Belongs to the cucumoviruses/bromoviruses 2A family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; U20218; AAC54616.1; -; Genomic_RNA.
DR   PIR; A71392; A71392.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR001788; RNA-dep_RNA_pol_vir-typ.
DR   InterPro; IPR007094; RNA_pol_PSvir.
DR   Pfam; PF00978; RdRP_2; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA replication;
KW   RNA-directed RNA polymerase; Transferase.
FT   CHAIN         1    858       RNA-directed RNA polymerase 2A.
FT                                /FTId=PRO_0000083275.
FT   DOMAIN      511    624       RdRp catalytic.
SQ   SEQUENCE   858 AA;  97045 MW;  AE0151CA566B4005 CRC64;
     MAFPAPAFSL ANLLNGTYGV DTPEEVERVR SEQREEAAAA CRNYKPLPAV DVGASVTEDA
     HSLRTPDGAP SEEVSVEFVT YGAEDYLEKS DDELFVAFET MVKPMCIGQL WCPAFNKCSF
     ISSIAMARAL LLVPNTPNRT MKCFEDLVAA IYTKSDFYYD DECEADDVQI NISSRDVPGY
     SFEPWSRTSG FEPPPICEAC NMIMYQCPCF DFNALKKSCA ERTFADDYVI EGLDGVVDNA
     TLLSNLGPFL VPVKCQYKQY PTPTVAIPPS LNRATDRVDI NLVQSICDST LPTHSNYDDS
     FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLN TGSGHKRVGT TKEVLTAIKK
     RNADVPELGD SVNLPRLSKA VAERFFIAYI NGNSLATSNF VNVVSNFHDY MEKWKSSGLS
     YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPVPAT ITYHKKGITS QFSPLFTALF
     ERFQRCLRER VILPVGKISS LEMSGFDVKN KYCLEIDLSK FDKSQGEFHL MIQEHILNGL
     GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAF TYFGNTIVTM AEFAWCYDTD
     QFEKLLFPGD DSLGFSVLPP VGDPSKFTTL FNMEAKVMEP AVPYICSKFL LSDEFGNTFS
     VPDPLREVQR LGTKKIPYSD NDEFLFAHFM SFVDRLKFLD RMTQSCIDQL SLFFELKYRK
     SGAEAALMLG AFKKYTANFQ SYKELYYSDR RQCELINSFS CVELRIERSS FIKQRKKKDG
     IERRRNDKRR TPTSPHGGGE ETETKVSQEE STGTMLQKSQ RESAFKSQTI PFPTVLSSRR
     FGIDRDVPPR ECGGIVRV
//