Reviewed, UniProtKB/Swiss-Prot Q66117 (ORF2A_CMVIX)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase 2A
      Short name=protein 2A
    EC=2.7.7.48

Gene names

Name:	RNA2
ORF Names:	ORF2a

Organism

Cucumber mosaic virus (strain Ixora) (CMV)

Taxonomic identifier

117114 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Bromoviridae › Cucumovirus

Virus host

Spinacia oleracea (Spinach) [TaxID: 3562]
Cucumis sativus (Cucumber) [TaxID: 3659]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]

Protein attributes

Sequence length	858 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-directed RNA polymerase.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Miscellaneous	The virus has a tripartite genome (RNA1, RNA2 and RNA3) and an encapsidated subgenomic RNA (RNA4) from which the coat protein is expressed. Virions are icosahedral, not enveloped, with 32 capsomers per nucleocapsid.
Sequence similarities	Belongs to the cucumoviruses/bromoviruses 2A family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 858	858	RNA-directed RNA polymerase 2A		PRO_0000083275
Regions
	Domain	511 – 624	114	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q66117-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: AE0151CA566B4005	FASTA	858	97,045
	10 20 30 40 50 60 MAFPAPAFSL ANLLNGTYGV DTPEEVERVR SEQREEAAAA CRNYKPLPAV DVGASVTEDA 70 80 90 100 110 120 HSLRTPDGAP SEEVSVEFVT YGAEDYLEKS DDELFVAFET MVKPMCIGQL WCPAFNKCSF 130 140 150 160 170 180 ISSIAMARAL LLVPNTPNRT MKCFEDLVAA IYTKSDFYYD DECEADDVQI NISSRDVPGY 190 200 210 220 230 240 SFEPWSRTSG FEPPPICEAC NMIMYQCPCF DFNALKKSCA ERTFADDYVI EGLDGVVDNA 250 260 270 280 290 300 TLLSNLGPFL VPVKCQYKQY PTPTVAIPPS LNRATDRVDI NLVQSICDST LPTHSNYDDS 310 320 330 340 350 360 FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLN TGSGHKRVGT TKEVLTAIKK 370 380 390 400 410 420 RNADVPELGD SVNLPRLSKA VAERFFIAYI NGNSLATSNF VNVVSNFHDY MEKWKSSGLS 430 440 450 460 470 480 YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPVPAT ITYHKKGITS QFSPLFTALF 490 500 510 520 530 540 ERFQRCLRER VILPVGKISS LEMSGFDVKN KYCLEIDLSK FDKSQGEFHL MIQEHILNGL 550 560 570 580 590 600 GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAF TYFGNTIVTM AEFAWCYDTD 610 620 630 640 650 660 QFEKLLFPGD DSLGFSVLPP VGDPSKFTTL FNMEAKVMEP AVPYICSKFL LSDEFGNTFS 670 680 690 700 710 720 VPDPLREVQR LGTKKIPYSD NDEFLFAHFM SFVDRLKFLD RMTQSCIDQL SLFFELKYRK 730 740 750 760 770 780 SGAEAALMLG AFKKYTANFQ SYKELYYSDR RQCELINSFS CVELRIERSS FIKQRKKKDG 790 800 810 820 830 840 IERRRNDKRR TPTSPHGGGE ETETKVSQEE STGTMLQKSQ RESAFKSQTI PFPTVLSSRR 850 FGIDRDVPPR ECGGIVRV

References

[1]	"The complete sequence of a cucumber mosaic virus from Ixora that is deficient in the replication of satellite RNAs." McGarvey P.B., Tousignant M., Geletka L., Cellini F., Kaper J.M. J. Gen. Virol. 76:2257-2270(1995) [PubMed: 7561763] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U20218 Genomic RNA. Translation: AAC54616.1.
PIR	A71392.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001788. RNA-dep_RNA_pol_vir-typ. IPR007094. RNA_pol_PSvir. [Graphical view]
Pfam	PF00978. RdRP_2. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ORF2A_CMVIX

Accession

Primary (citable) accession number: Q66117

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)