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Q66104

- P19_CIRV

UniProt

Q66104 - P19_CIRV

Protein

RNA silencing suppressor p19

Gene

ORF4

Organism
Carnation Italian ringspot virus (CIRV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. Binds to short interfering RNAs (siRNAs) with high affinity. Acts as a molecular caliper to specifically select siRNAs based on the length of the duplex region of the RNA.2 Publications

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Suppressor of RNA silencing

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA silencing suppressor p19
    Alternative name(s):
    19 kDa symptom severity modulator
    Gene namesi
    ORF Names:ORF4
    OrganismiCarnation Italian ringspot virus (CIRV)
    Taxonomic identifieri39443 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageTombusviridaeTombusvirus
    Virus hostiDianthus caryophyllus (Carnation) (Clove pink) [TaxID: 3570]

    Subcellular locationi

    GO - Cellular componenti

    1. virion Source: InterPro

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi39 – 391W → G: Complete loss of silencing suppression. 1 Publication
    Mutagenesisi42 – 421W → G: Complete loss of silencing suppression. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 172172RNA silencing suppressor p19PRO_0000222872Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    Secondary structure

    1
    172
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi10 – 178
    Beta strandi29 – 324
    Helixi39 – 457
    Beta strandi57 – 659
    Beta strandi68 – 758
    Helixi80 – 867
    Helixi87 – 893
    Helixi92 – 1009
    Beta strandi109 – 1168
    Beta strandi119 – 1268
    Helixi127 – 1293
    Helixi130 – 14516

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1RPUX-ray2.50A/B1-172[»]
    ProteinModelPortaliQ66104.
    SMRiQ66104. Positions 9-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ66104.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tombusvirus protein p19 family.Curated

    Family and domain databases

    InterProiIPR004905. Tombusvirus_p19.
    [Graphical view]
    PfamiPF03220. Tombus_P19. 1 hit.
    [Graphical view]
    ProDomiPD005743. Tombus_P19. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF103145. SSF103145. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q66104-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERAIQGNDT REQANGERWD GGSGGITSPF KLPDESPSWT EWRLYNDETN    50
    SNQDNPLGFK ESWGFGKVVF KRYLRYDRTE ASLHRVLGSW TGDSVNYAAS 100
    RFLGANQVGC TYSIRFRGVS VTISGGSRTL QHLCEMAIRS KQELLQLTPV 150
    EVESNVSRGC PEGIETFKKE SE 172
    Length:172
    Mass (Da):19,335
    Last modified:November 1, 1996 - v1
    Checksum:iEF81C6386E06C3C4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85215 Genomic RNA. Translation: CAA59481.1.
    RefSeqiNP_612584.1. NC_003500.2.

    Genome annotation databases

    GeneIDi935442.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85215 Genomic RNA. Translation: CAA59481.1 .
    RefSeqi NP_612584.1. NC_003500.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1RPU X-ray 2.50 A/B 1-172 [» ]
    ProteinModelPortali Q66104.
    SMRi Q66104. Positions 9-152.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 935442.

    Miscellaneous databases

    EvolutionaryTracei Q66104.

    Family and domain databases

    InterProi IPR004905. Tombusvirus_p19.
    [Graphical view ]
    Pfami PF03220. Tombus_P19. 1 hit.
    [Graphical view ]
    ProDomi PD005743. Tombus_P19. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF103145. SSF103145. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and complete nucleotide sequence of carnation Italian ringspot tombusvirus genomic and defective interfering RNAs."
      Rubino L., Burgyan J., Russo M.
      Arch. Virol. 140:2027-2039(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Recognition mechanism of siRNA by viral p19 suppressor of RNA silencing: a molecular dynamics study."
      Xia Z., Zhu Z., Zhu J., Zhou R.
      Biophys. J. 96:1761-1769(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF TRP-39 AND TRP-42.
    3. "Size selective recognition of siRNA by an RNA silencing suppressor."
      Vargason J.M., Szittya G., Burgyan J., Tanaka Hall T.M.
      Cell 115:799-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION.

    Entry informationi

    Entry nameiP19_CIRV
    AccessioniPrimary (citable) accession number: Q66104
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3