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Protein

RNA silencing suppressor p19

Gene

ORF4

Organism
Carnation Italian ringspot virus (CIRV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. Binds to short interfering RNAs (siRNAs) with high affinity. Acts as a molecular caliper to specifically select siRNAs based on the length of the duplex region of the RNA.2 Publications

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Suppressor of RNA silencing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA silencing suppressor p19
Alternative name(s):
19 kDa symptom severity modulator
Gene namesi
ORF Names:ORF4
OrganismiCarnation Italian ringspot virus (CIRV)
Taxonomic identifieri39443 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageTombusviridaeTombusvirus
Virus hostiDianthus caryophyllus (Carnation) (Clove pink) [TaxID: 3570]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39W → G: Complete loss of silencing suppression. 1 Publication1
Mutagenesisi42W → G: Complete loss of silencing suppression. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002228721 – 172RNA silencing suppressor p19Add BLAST172

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1172
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 17Combined sources8
Beta strandi29 – 32Combined sources4
Helixi39 – 45Combined sources7
Beta strandi57 – 65Combined sources9
Beta strandi68 – 75Combined sources8
Helixi80 – 86Combined sources7
Helixi87 – 89Combined sources3
Helixi92 – 100Combined sources9
Beta strandi109 – 116Combined sources8
Beta strandi119 – 126Combined sources8
Helixi127 – 129Combined sources3
Helixi130 – 145Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RPUX-ray2.50A/B1-172[»]
ProteinModelPortaliQ66104.
SMRiQ66104.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ66104.

Family & Domainsi

Sequence similaritiesi

Belongs to the tombusvirus protein p19 family.Curated

Family and domain databases

InterProiIPR004905. Tombusvirus_p19.
[Graphical view]
PfamiPF03220. Tombus_P19. 1 hit.
[Graphical view]
ProDomiPD005743. Tombus_P19. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF103145. SSF103145. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66104-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERAIQGNDT REQANGERWD GGSGGITSPF KLPDESPSWT EWRLYNDETN
60 70 80 90 100
SNQDNPLGFK ESWGFGKVVF KRYLRYDRTE ASLHRVLGSW TGDSVNYAAS
110 120 130 140 150
RFLGANQVGC TYSIRFRGVS VTISGGSRTL QHLCEMAIRS KQELLQLTPV
160 170
EVESNVSRGC PEGIETFKKE SE
Length:172
Mass (Da):19,335
Last modified:November 1, 1996 - v1
Checksum:iEF81C6386E06C3C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85215 Genomic RNA. Translation: CAA59481.1.
RefSeqiNP_612584.1. NC_003500.2.

Genome annotation databases

GeneIDi935442.
KEGGivg:935442.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85215 Genomic RNA. Translation: CAA59481.1.
RefSeqiNP_612584.1. NC_003500.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RPUX-ray2.50A/B1-172[»]
ProteinModelPortaliQ66104.
SMRiQ66104.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi935442.
KEGGivg:935442.

Miscellaneous databases

EvolutionaryTraceiQ66104.

Family and domain databases

InterProiIPR004905. Tombusvirus_p19.
[Graphical view]
PfamiPF03220. Tombus_P19. 1 hit.
[Graphical view]
ProDomiPD005743. Tombus_P19. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF103145. SSF103145. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiP19_CIRV
AccessioniPrimary (citable) accession number: Q66104
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.