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Q66104

- P19_CIRV

UniProt

Q66104 - P19_CIRV

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Protein

RNA silencing suppressor p19

Gene

ORF4

Organism
Carnation Italian ringspot virus (CIRV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. Binds to short interfering RNAs (siRNAs) with high affinity. Acts as a molecular caliper to specifically select siRNAs based on the length of the duplex region of the RNA.2 Publications

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Suppressor of RNA silencing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA silencing suppressor p19
Alternative name(s):
19 kDa symptom severity modulator
Gene namesi
ORF Names:ORF4
OrganismiCarnation Italian ringspot virus (CIRV)
Taxonomic identifieri39443 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageTombusviridaeTombusvirus
Virus hostiDianthus caryophyllus (Carnation) (Clove pink) [TaxID: 3570]

Subcellular locationi

GO - Cellular componenti

  1. virion Source: InterPro
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi39 – 391W → G: Complete loss of silencing suppression. 1 Publication
Mutagenesisi42 – 421W → G: Complete loss of silencing suppression. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172RNA silencing suppressor p19PRO_0000222872Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
172
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 178Combined sources
Beta strandi29 – 324Combined sources
Helixi39 – 457Combined sources
Beta strandi57 – 659Combined sources
Beta strandi68 – 758Combined sources
Helixi80 – 867Combined sources
Helixi87 – 893Combined sources
Helixi92 – 1009Combined sources
Beta strandi109 – 1168Combined sources
Beta strandi119 – 1268Combined sources
Helixi127 – 1293Combined sources
Helixi130 – 14516Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RPUX-ray2.50A/B1-172[»]
ProteinModelPortaliQ66104.
SMRiQ66104. Positions 9-152.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ66104.

Family & Domainsi

Sequence similaritiesi

Belongs to the tombusvirus protein p19 family.Curated

Family and domain databases

InterProiIPR004905. Tombusvirus_p19.
[Graphical view]
PfamiPF03220. Tombus_P19. 1 hit.
[Graphical view]
ProDomiPD005743. Tombus_P19. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF103145. SSF103145. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66104-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERAIQGNDT REQANGERWD GGSGGITSPF KLPDESPSWT EWRLYNDETN
60 70 80 90 100
SNQDNPLGFK ESWGFGKVVF KRYLRYDRTE ASLHRVLGSW TGDSVNYAAS
110 120 130 140 150
RFLGANQVGC TYSIRFRGVS VTISGGSRTL QHLCEMAIRS KQELLQLTPV
160 170
EVESNVSRGC PEGIETFKKE SE
Length:172
Mass (Da):19,335
Last modified:November 1, 1996 - v1
Checksum:iEF81C6386E06C3C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85215 Genomic RNA. Translation: CAA59481.1.
RefSeqiNP_612584.1. NC_003500.2.

Genome annotation databases

GeneIDi935442.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85215 Genomic RNA. Translation: CAA59481.1 .
RefSeqi NP_612584.1. NC_003500.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1RPU X-ray 2.50 A/B 1-172 [» ]
ProteinModelPortali Q66104.
SMRi Q66104. Positions 9-152.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 935442.

Miscellaneous databases

EvolutionaryTracei Q66104.

Family and domain databases

InterProi IPR004905. Tombusvirus_p19.
[Graphical view ]
Pfami PF03220. Tombus_P19. 1 hit.
[Graphical view ]
ProDomi PD005743. Tombus_P19. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF103145. SSF103145. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and complete nucleotide sequence of carnation Italian ringspot tombusvirus genomic and defective interfering RNAs."
    Rubino L., Burgyan J., Russo M.
    Arch. Virol. 140:2027-2039(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Recognition mechanism of siRNA by viral p19 suppressor of RNA silencing: a molecular dynamics study."
    Xia Z., Zhu Z., Zhu J., Zhou R.
    Biophys. J. 96:1761-1769(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TRP-39 AND TRP-42.
  3. "Size selective recognition of siRNA by an RNA silencing suppressor."
    Vargason J.M., Szittya G., Burgyan J., Tanaka Hall T.M.
    Cell 115:799-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION.

Entry informationi

Entry nameiP19_CIRV
AccessioniPrimary (citable) accession number: Q66104
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3