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Q660T5 (SYM_BORGA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:BG0600
OrganismBorrelia garinii [Complete proteome] [HAMAP]
Taxonomic identifier29519 [NCBI]
Taxonomic lineageBacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length724 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Homodimer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 724724Methionine--tRNA ligase HAMAP MF_00098
PRO_0000139108

Regions

Domain560 – 665106tRNA-binding
Motif12 – 2211"HIGH" region HAMAP MF_00098
Motif330 – 3345"KMSKS" region HAMAP MF_00098

Sites

Metal binding1431Zinc By similarity
Metal binding1461Zinc By similarity
Metal binding1551Zinc By similarity
Metal binding1581Zinc By similarity
Binding site3331ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q660T5 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 75C5C1D4169FC999

FASTA72484,240
        10         20         30         40         50         60 
MKKMNLVTAA LPYVNNIPHL GNLVQVLSAD AFARYSKMSG IETLYICGTD EYGTATETKA 

        70         80         90        100        110        120 
LIEKTSPLEL CNKYYKIHKS IYKWFNIEFD IFGRTTNKHH QDIVQNFFLK LEKNGYIKER 

       130        140        150        160        170        180 
ETEQFFCNKD SIFLADRYVI GECPECQSIA KGDQCENCSK LLNPIDLINP KCIICKNKPV 

       190        200        210        220        230        240 
LKKTNHLYID LPKIKRKLEK WIKNSTTSKN WNTNALKMTN AFLRDGLKER AITRDLKWGI 

       250        260        270        280        290        300 
PVPKKGYENK VFYVWFDAPI GYISITKNLV KNWESWWKNN EQVNLVQFIG KDNILFHTVI 

       310        320        330        340        350        360 
FPSIEIGSKE NWTILNQLSS SEYLNYENLK FSKSEGTGIF GNDVITTGIP SDVWRFYIYY 

       370        380        390        400        410        420 
NRPEKSDFQF MWQDLMERVN TELIDNFSNL VNRVLTFQKK FFGDVIETIE IQNKFWKQII 

       430        440        450        460        470        480 
PKYNKILNLF KKTELKSALK EILKISSLGN KIFQDNEPWK RKDSSPQETK ELISNLIYLI 

       490        500        510        520        530        540 
RDLSILMMPF IPETSKKIQQ FFGNSYQFST KILGTKSGIK RIEFTEILFN KLEQKKINNL 

       550        560        570        580        590        600 
KLKYSGEKNM EEKEQPENLF REKVLLKVVK INKIERNPEA KNLFILKLDD GTNENKQIVS 

       610        620        630        640        650        660 
SLEGYYTEKE LLGKHIIIVD NLKPAKFRGI KSEGMLIAAE DKNKNFKIII VEDSIKNPIP 

       670        680        690        700        710        720 
GERIILENDQ NKDLICPPKI DINKFSKANI VAKDGELKIN GINLILEHSK NKILSKDIPN 


GIVC

« Hide

References

[1]"Comparative analysis of the Borrelia garinii genome."
Gloeckner G., Lehmann R., Romualdi A., Pradella S., Schulte-Spechtel U., Schilhabel M., Wilske B., Suehnel J., Platzer M.
Nucleic Acids Res. 32:6038-6046(2004) [PubMed: 15547252] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PBi.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000013 Genomic DNA. Translation: AAU07436.1.
RefSeqYP_073028.1. NC_006156.1.

3D structure databases

ProteinModelPortalQ660T5.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBORT00000008021; EBBORP00000007396; EBBORG00000008020.
GeneID2957737.
GenomeReviewsGene locus BG0600 in contig CP000013_GR.
KEGGbga:BG0600.
PATRIC20566765. VBIBorGar102262_0693.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000007495.
HOGENOMHBG721667.
OMAEEKTRNV.
PhylomeDBQ660T5.
ProtClustDBPRK00133.

Enzyme and pathway databases

BioCycBGAR290434:BG0600-MONOMER.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
TIGR00399. MetG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_BORGA
AccessionPrimary (citable) accession number: Q660T5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 11, 2004
Last modified: January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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