Q660S4 (MURB_BORGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BG0611

Organism

Borrelia garinii [Complete proteome] [HAMAP]

Taxonomic identifier

29519 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000224668
Regions
	Domain	28 – 194	167	FAD-binding PCMH-type
Sites
	Active site	223	1	Proton donor By similarity
	Active site	295	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q660S4 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: A3FCBBAAFE346639	FASTA	306	34,753
	10 20 30 40 50 60 MIKRLNNFFE KINIKPQTKN LINYTTYKIG NISKLFLIPK NIQEAENIFK AAIEEKITLF 70 80 90 100 110 120 ILGGGSNILV NDKEELDFPI IYTGHLNKIE VHDNKIVAEC GANFENLCKI ALNSSLSGLE 130 140 150 160 170 180 FIYGLPGTLG GAVWMNARCF GNEISEILKK ITFINDKGKT ICKEFKKEDF KYKVSPFQNK 190 200 210 220 230 240 NFFILKTELN LKKENKKIIE EKMNKNKQAR INKGHYLFPS SGSTFKNNKS FLRPSGQIIE 250 260 270 280 290 300 ECKLKGLSVG GAEVSKYHGN FIININNATS NDIKSLIEKV KTEVYSKTGL LLEEEVLYIG FKNQKS

References

[1]

"Comparative analysis of the Borrelia garinii genome." Gloeckner G., Lehmann R., Romualdi A., Pradella S., Schulte-Spechtel U., Schilhabel M., Wilske B., Suehnel J., Platzer M. Nucleic Acids Res. 32:6038-6046(2004) [PubMed: 15547252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PBi.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000013 Genomic DNA. Translation: AAU07447.1.
RefSeq	YP_073039.1. NC_006156.1.
3D structure databases
ProteinModelPortal	Q660S4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000007426; EBBORP00000006801; EBBORG00000007425.
GeneID	2957815.
GenomeReviews	Gene locus BG0611 in contig CP000013_GR.
KEGG	bga:BG0611.
PATRIC	20566791. VBIBorGar102262_0706.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007374.
HOGENOM	HBG686573.
OMA	KITFIND.
PhylomeDB	Q660S4.
ProtClustDB	PRK14650.
Enzyme and pathway databases
BioCyc	BGAR290434:BG0611-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BORGA

Accession

Primary (citable) accession number: Q660S4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program