Q660H3 (TRMD_BORGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BG0721

Organism

Borrelia garinii [Complete proteome] [HAMAP]

Taxonomic identifier

29519 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060340
Regions
	Region	130 – 135	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	110	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q660H3 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: F62D543EE11EB5ED	FASTA	239	27,328
	10 20 30 40 50 60 MKFTVISLFP AIIKPFFENS IMKKAINKGI VSFELIDVRD FSKDKHKRCD DLSYGGGAGM 70 80 90 100 110 120 VLKAEPMSFA LEHVEATKKT TIFLSPSGIK YTQELAYSLS KKEEIVIICG RYEGIDQRII 130 140 150 160 170 180 DLYVDFEISI GDYVLSSGEI AALVLIDSVY RLLDGVINPN SLLEESFGMK NGLLEYPHYT 190 200 210 220 230 RPYNFMGIKV PEVLVSGHHE NIKNWRLFKA REKTKKNRYD LYLKYLEIIG EDNGFDKKN

References

[1]

"Comparative analysis of the Borrelia garinii genome." Gloeckner G., Lehmann R., Romualdi A., Pradella S., Schulte-Spechtel U., Schilhabel M., Wilske B., Suehnel J., Platzer M. Nucleic Acids Res. 32:6038-6046(2004) [PubMed: 15547252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PBi.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000013 Genomic DNA. Translation: AAU07548.1.
RefSeq	YP_073140.1. NC_006156.1.
3D structure databases
ProteinModelPortal	Q660H3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000007660; EBBORP00000007035; EBBORG00000007659.
GeneID	2957212.
GenomeReviews	Gene locus BG0721 in contig CP000013_GR.
KEGG	bga:BG0721.
PATRIC	20567003. VBIBorGar102262_0804.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007285.
HOGENOM	HBG285805.
OMA	NRYDLYL.
PhylomeDB	Q660H3.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	BGAR290434:BG0721-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BORGA

Accession

Primary (citable) accession number: Q660H3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program