Q660F3 (SYT_BORGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BG0742

Organism

Borrelia garinii [Complete proteome] [HAMAP]

Taxonomic identifier

29519 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 582	582	Threonine--tRNA ligase HAMAP MF_00184		PRO_0000100946
Regions
	Region	185 – 478	294	Catalytic HAMAP MF_00184
Sites
	Metal binding	278	1	Zinc; catalytic By similarity
	Metal binding	329	1	Zinc; catalytic By similarity
	Metal binding	455	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q660F3 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: B634C39182F942F6	FASTA	582	68,163
	10 20 30 40 50 60 MSKDLYKEDI LYKKRHSIAH VMAEAVCDLF PNTKIAIGPP IKDGFYYDFE FKNPITEDSL 70 80 90 100 110 120 LDIENRMREI LKTGSSFEKE IISLEQALEI FKDEPYKIDL IKNFDLQNEI SIYKSHNFID 130 140 150 160 170 180 LCRGPHVDNM NKIDPKAFKL TGIAGAYWRG NEKNTMLTRI YGTLWNNEKE LRSYLNLREE 190 200 210 220 230 240 IKKRDHRKLG KELDLFSIHE EIGPGLIFFH PNGAKIRALI EDFWREEHSK NGYDILFTPH 250 260 270 280 290 300 VGKSWLWQTS GHLNFYKDSM FEKIEMDKSD YYLKPMNCPF HIAIYNTGKH SYRDLPFRWA 310 320 330 340 350 360 ELGTVYRYEK IGALHGMMRA RGFTQDDAHI ICTHSQVVDE IKEVLRFAIY MWSKFGFNSL 370 380 390 400 410 420 KAYLSTKPDK SVGNNSDWEM SLKVLKEALS DFEVPYEIDK GGGAFYGPKI DLKIVDSLER 430 440 450 460 470 480 EWQMSTIQFD FNLPERFNMT YTSEDGKEKR PFMIHRALLG SIERFFGILV EHYGGAFPLW 490 500 510 520 530 540 LSHVQAVIIP VNNIVEDYTI KVFNKFKNAG IRIKLDNSSS RMNAKIREYQ AKKIPYMFII 550 560 570 580 GEREAIEERI SIRTRTNEQI NGIELDEALK LILLKVRDKE IS

References

[1]

"Comparative analysis of the Borrelia garinii genome." Gloeckner G., Lehmann R., Romualdi A., Pradella S., Schulte-Spechtel U., Schilhabel M., Wilske B., Suehnel J., Platzer M. Nucleic Acids Res. 32:6038-6046(2004) [PubMed: 15547252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PBi.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000013 Genomic DNA. Translation: AAU07568.1.
RefSeq	YP_073160.1. NC_006156.1.
3D structure databases
ProteinModelPortal	Q660F3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000007644; EBBORP00000007019; EBBORG00000007643.
GeneID	2957754.
GenomeReviews	Gene locus BG0742 in contig CP000013_GR.
KEGG	bga:BG0742.
PATRIC	20567049. VBIBorGar102262_0825.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007426.
HOGENOM	HBG352811.
OMA	MIIRNIL.
ProtClustDB	PRK12305.
Enzyme and pathway databases
BioCyc	BGAR290434:BG0742-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BORGA

Accession

Primary (citable) accession number: Q660F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program