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Q66096 (RDRP_CRSVL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase

EC=2.7.7.48
Alternative name(s):
Protein p88
Gene names
ORF Names:ORF1
OrganismCarnation ringspot virus (isolate Lommel) (CRSV) [Complete proteome]
Taxonomic identifier652597 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageTombusviridaeDianthovirus
Virus hostDianthus barbatus [TaxID: 278075]
Dianthus caryophyllus (Carnation) (Clove pink) [TaxID: 3570]

Protein attributes

Sequence length769 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Probable polymerase.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sequence similarities

Belongs to the tombusviridae RNA polymerase family.

Contains 1 RdRp catalytic domain.

Sequence caution

The sequence AAA20212.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processViral RNA replication
   Coding sequence diversityRibosomal frameshifting
   LigandNucleotide-binding
   Molecular functionNucleotidyltransferase
RNA-directed RNA polymerase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processviral RNA genome replication

Inferred from electronic annotation. Source: InterPro

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nucleotide binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform 88 kDa protein (identifier: Q66096-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by ribosomal frameshifting between codons 236 and 237.
Isoform protein p27 (identifier: Q66096-2)

The sequence of this isoform differs from the canonical sequence as follows:
     237-767: Missing.
Note: Produced by conventional translation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 769769RNA-directed RNA polymerase
PRO_0000398304

Regions

Domain463 – 579117RdRp catalytic

Natural variations

Alternative sequence237 – 767531Missing in isoform protein p27.
VSP_039744

Sequences

Sequence LengthMass (Da)Tools
Isoform 88 kDa protein [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: 0B9FBFD6D28949AA

FASTA76987,776
        10         20         30         40         50         60 
MAIFELFSFD IDKLLVWVSK FSPGKILSAI CRLGLDSWNN FRKWFWGLNF DKHEWAVDHF 

        70         80         90        100        110        120 
MPLMPRFSSD MDEVAKRIVT PKSKPKLEDC LEIDTAVEEC FNEECFEPQE DGSMKLKRVA 

       130        140        150        160        170        180 
APQQIKRVRT GMIEDAISAV EARIRNRHMI MGDDMGLVDE AAVRATAMDI CGEYKINEHH 

       190        200        210        220        230        240 
TRCIIYAAAY RAMTPDQESI DATKMAYNPK SQARRDLVSI LRKNISFGGF KSLEDFLSAP 

       250        260        270        280        290        300 
VSFPVEDAPY QILGIPEIKV ADKRASRVCK FKRVVGLPSL SAGQSVCVHK TSLHNMIVSL 

       310        320        330        340        350        360 
EQRVFRVKNE TGEFVVPPQP SKGAFDSISY FREAWKKKLY SKGPVVKSSI DDVVACYSSE 

       370        380        390        400        410        420 
KKKLYQKGAA TLSHRPLHWR DSKVRAFIKV EKLECDKKAP VPRTIQPRSK RYNLCIGRYL 

       430        440        450        460        470        480 
RLNEKRMLDA IDAVFGEKTV LSGLDNKAQG RAIAKKWSKY ESPIGIGLDA SRFDQHCSKD 

       490        500        510        520        530        540 
ALKFEHSFYR ECFPDDKTSP DLLDWQLENE GSALMPTESL VKYRTRCRMS GDINTGLGNK 

       550        560        570        580        590        600 
ILMCSMVHAY LKEVGVNASL ANNGDDCVLF CEKGDFNRIN DSLREWFLCR GFNMVVEEPV 

       610        620        630        640        650        660 
ECLERVVFCR SQPVCVATKW AMVRQLGSLS RDCFSTQNWL NPTTFRDAMN ALGQCNGIIN 

       670        680        690        700        710        720 
DGVPVHMAQA KRMYAAGGNR KFDLKALHKQ MEYSWRDRLG ARTNLLWSEV EDSTRLSYFR 

       730        740        750        760 
AFGIEPAVQR IVEGYFSESK ISEEGRQTNF LPTHYSRLHK DLLVPRYLN 

« Hide

Isoform protein p27 [UniParc].

Checksum: 02A1E8922B798705
Show »

FASTA23827,386

References

[1]"Nucleotide sequence of carnation ringspot dianthovirus RNA-1."
Ryabov E.V., Generozov E.V., Kendall T.L., Lommel S.A., Zavriev S.K.
J. Gen. Virol. 75:243-247(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]Sit T.L., Lommel S.A.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L18870 Genomic RNA. Translation: AAA20211.1.
L18870 Genomic RNA. Translation: AAA20212.1. Sequence problems.

3D structure databases

ProteinModelPortalQ66096.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
[Graphical view]
PfamPF00998. RdRP_3. 1 hit.
[Graphical view]
PROSITEPS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRDRP_CRSVL
AccessionPrimary (citable) accession number: Q66096
Secondary accession number(s): Q66097
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 5, 2010
Last modified: April 16, 2014
This is version 34 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families