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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Borrelia garinii (strain PBi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi486 – 4861MagnesiumUniRule annotation
Metal bindingi492 – 4921MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciBGAR290434:BG0831-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:BG0831
OrganismiBorrelia garinii (strain PBi)
Taxonomic identifieri290434 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliaBorrelia burgdorferi group
ProteomesiUP000002276 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Polyribonucleotide nucleotidyltransferasePRO_0000329541Add
BLAST

Proteomic databases

PRIDEiQ65ZW8.

Interactioni

Protein-protein interaction databases

STRINGi290434.BG0831.

Structurei

3D structure databases

ProteinModelPortaliQ65ZW8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini553 – 61260KHUniRule annotationAdd
BLAST
Domaini622 – 71695S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218326.
KOiK00962.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 2 hits.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65ZW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKILKLKIG RDELVFETGF MAKQANGSVL ATYGGSSVLA TVCCSNNVRE
60 70 80 90 100
DLDFVPLSVE YNEKYYAAGK IPGGFIKREG KPKDKEILVS RLIDRPMRPL
110 120 130 140 150
FDKRFGREIQ VIPTTLATDQ LNPPDIVGMN AAFTAVFLSD IPFNGPIAAV
160 170 180 190 200
RMVYLNGKFI VNPSFEEIHD SDLDIVVAGS LNGITMVEGG ANEVSEDILL
210 220 230 240 250
SAIDGAHEYI KQICNAQKEF LDIVGEKEKL PLAFEEKIFE FKEELKDFIY
260 270 280 290 300
TDLKEACFVK GKLNRDKAIT LLRNKSYEHF SSLEKLTDSN ESLFYKAFDD
310 320 330 340 350
FEREIVRNSI LNDKIRTDGR TPNEIRDIIA EVDILSRTHG SALFTRGETQ
360 370 380 390 400
ALAVTTLGTS IDEQIMDDID GDKRLNFMLH YNFPPFSVGE TGRLMTGRRE
410 420 430 440 450
IGHGHLAQRA LESMVPGKND FPYTIRVVSE ILESNGSSSM ATVCAGSMSL
460 470 480 490 500
MSAGVPVKRQ VAGIAMGLIS EGDKYVVLSD ILGEEDHLGD MDFKVAGTKN
510 520 530 540 550
GITGFQMDIK IENVTKHLMR DALEQARIGR MHILSVMDTV ISDSRVGISK
560 570 580 590 600
YAPKIVQLQI DIDKISLVIG STGKTVKAIT DEFEVKVQIE QNGKIILFGD
610 620 630 640 650
DDFKMQKAKE RIESIVREPK VGEIYEGIVK KINSFGAFIE LTPTKEGFLS
660 670 680 690 700
TRLKSRDNKY GSGRFGTGNR YPRFGGGDNI RGNVGLVRPP KLEEGQQIKV
710 720
KIIDIDKFGK IDLEVVRDKD Y
Length:721
Mass (Da):80,286
Last modified:April 29, 2008 - v2
Checksum:i4002F1A9124CC131
GO

Sequence cautioni

The sequence AAU07653.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000013 Genomic DNA. Translation: AAU07653.1. Different initiation.
RefSeqiWP_044007938.1. NC_006156.1.

Genome annotation databases

EnsemblBacteriaiAAU07653; AAU07653; BG0831.
KEGGibga:BG0831.
PATRICi20567235. VBIBorGar102262_0914.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000013 Genomic DNA. Translation: AAU07653.1. Different initiation.
RefSeqiWP_044007938.1. NC_006156.1.

3D structure databases

ProteinModelPortaliQ65ZW8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290434.BG0831.

Proteomic databases

PRIDEiQ65ZW8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU07653; AAU07653; BG0831.
KEGGibga:BG0831.
PATRICi20567235. VBIBorGar102262_0914.

Phylogenomic databases

HOGENOMiHOG000218326.
KOiK00962.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciBGAR290434:BG0831-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 2 hits.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PBi.

Entry informationi

Entry nameiPNP_BORGA
AccessioniPrimary (citable) accession number: Q65ZW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: July 22, 2015
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.