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Q65ZW8

- PNP_BORGA

UniProt

Q65ZW8 - PNP_BORGA

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Borrelia garinii (strain PBi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 2 (29 Apr 2008)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi486 – 4861MagnesiumUniRule annotation
    Metal bindingi492 – 4921MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciBGAR290434:BG0831-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:BG0831
    OrganismiBorrelia garinii (strain PBi)
    Taxonomic identifieri290434 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
    ProteomesiUP000002276: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 721721Polyribonucleotide nucleotidyltransferasePRO_0000329541Add
    BLAST

    Proteomic databases

    PRIDEiQ65ZW8.

    Interactioni

    Protein-protein interaction databases

    STRINGi290434.BG0831.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65ZW8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini553 – 61260KHUniRule annotationAdd
    BLAST
    Domaini622 – 71695S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000218326.
    KOiK00962.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 2 hits.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q65ZW8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKILKLKIG RDELVFETGF MAKQANGSVL ATYGGSSVLA TVCCSNNVRE    50
    DLDFVPLSVE YNEKYYAAGK IPGGFIKREG KPKDKEILVS RLIDRPMRPL 100
    FDKRFGREIQ VIPTTLATDQ LNPPDIVGMN AAFTAVFLSD IPFNGPIAAV 150
    RMVYLNGKFI VNPSFEEIHD SDLDIVVAGS LNGITMVEGG ANEVSEDILL 200
    SAIDGAHEYI KQICNAQKEF LDIVGEKEKL PLAFEEKIFE FKEELKDFIY 250
    TDLKEACFVK GKLNRDKAIT LLRNKSYEHF SSLEKLTDSN ESLFYKAFDD 300
    FEREIVRNSI LNDKIRTDGR TPNEIRDIIA EVDILSRTHG SALFTRGETQ 350
    ALAVTTLGTS IDEQIMDDID GDKRLNFMLH YNFPPFSVGE TGRLMTGRRE 400
    IGHGHLAQRA LESMVPGKND FPYTIRVVSE ILESNGSSSM ATVCAGSMSL 450
    MSAGVPVKRQ VAGIAMGLIS EGDKYVVLSD ILGEEDHLGD MDFKVAGTKN 500
    GITGFQMDIK IENVTKHLMR DALEQARIGR MHILSVMDTV ISDSRVGISK 550
    YAPKIVQLQI DIDKISLVIG STGKTVKAIT DEFEVKVQIE QNGKIILFGD 600
    DDFKMQKAKE RIESIVREPK VGEIYEGIVK KINSFGAFIE LTPTKEGFLS 650
    TRLKSRDNKY GSGRFGTGNR YPRFGGGDNI RGNVGLVRPP KLEEGQQIKV 700
    KIIDIDKFGK IDLEVVRDKD Y 721
    Length:721
    Mass (Da):80,286
    Last modified:April 29, 2008 - v2
    Checksum:i4002F1A9124CC131
    GO

    Sequence cautioni

    The sequence AAU07653.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000013 Genomic DNA. Translation: AAU07653.1. Different initiation.
    RefSeqiYP_073245.1. NC_006156.1.

    Genome annotation databases

    EnsemblBacteriaiAAU07653; AAU07653; BG0831.
    GeneIDi2957582.
    KEGGibga:BG0831.
    PATRICi20567235. VBIBorGar102262_0914.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000013 Genomic DNA. Translation: AAU07653.1 . Different initiation.
    RefSeqi YP_073245.1. NC_006156.1.

    3D structure databases

    ProteinModelPortali Q65ZW8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290434.BG0831.

    Proteomic databases

    PRIDEi Q65ZW8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU07653 ; AAU07653 ; BG0831 .
    GeneIDi 2957582.
    KEGGi bga:BG0831.
    PATRICi 20567235. VBIBorGar102262_0914.

    Phylogenomic databases

    HOGENOMi HOG000218326.
    KOi K00962.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci BGAR290434:BG0831-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 2 hits.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PBi.

    Entry informationi

    Entry nameiPNP_BORGA
    AccessioniPrimary (citable) accession number: Q65ZW8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 66 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3