Reviewed, UniProtKB/Swiss-Prot Q65ZV7 (MURC_BORGA)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BG0842

Organism

Borrelia garinii [Complete proteome] [HAMAP]

Taxonomic identifier

29519 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182062
Regions
	Nucleotide binding	121 – 127	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q65ZV7-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 2C2B65BC5B40CAF8	FASTA	468	53,886
	10 20 30 40 50 60 MKVDFDSLNN IFFVGIKGSG VCSLACFLNS KGYFVEGVDV PDKFYTDDIL NNNEISYYEN 70 80 90 100 110 120 IYEFSLKELD RSFDLIVYSS AYDKDGLQVL LEAKELNIPI LSYSEVLGEL SRKHYSIGIA 130 140 150 160 170 180 GSHGKTTTTA FLGILFDKLG LNPNVIVGSS VKDFGDNSAI AGISDIFIVE TCEYKKHFLY 190 200 210 220 230 240 FSPNMLILTN VDYEHVDFFK NYEALEDAFL QYINNLKKNG ILIINSDDKN LLKIKSQINR 250 260 270 280 290 300 EDINIFSFGS RDLSNFQISN IVVKNEYFCF SFLGLYNIEL RTVLFHNILN FSAALLALNL 310 320 330 340 350 360 FLESNGKSIF DFEEVVKRVA KNYSGIKRRS ELIKEKKGVI YMDDYAHHPR EIRATLFGIK 370 380 390 400 410 420 NFYKNKRIIL DFMPHTFTRT KEFFDDFVEV LNIADVLILH NIYLSNRENF NPDELSFKLF 430 440 450 460 LNIKKINKNT YFFKDVKDSI NFIKSLLISG DLFITMGAGN NFILHDFL

References

[1]

"Comparative analysis of the Borrelia garinii genome." Gloeckner G., Lehmann R., Romualdi A., Pradella S., Schulte-Spechtel U., Schilhabel M., Wilske B., Suehnel J., Platzer M. Nucleic Acids Res. 32:6038-6046(2004) [PubMed: 15547252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PBi.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000013 Genomic DNA. Translation: AAU07664.1.
RefSeq	YP_073256.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2957638.
GenomeReviews	Gene locus BG0842 in contig CP000013_GR.
KEGG	bga:BG0842.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65ZV7.
OMA	EWMVVEA.
Enzyme and pathway databases
BioCyc	BGAR290434:BG0842-MON.
BRENDA	6.3.2.8. 294970.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BORGA

Accession

Primary (citable) accession number: Q65ZV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)