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Q65ZU1

- SYI_BORGA

UniProt

Q65ZU1 - SYI_BORGA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Borrelia garinii (strain PBi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei597 – 5971ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBGAR290434:BG0858-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BG0858
    OrganismiBorrelia garinii (strain PBi)
    Taxonomic identifieri290434 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
    ProteomesiUP000002276: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10421042Isoleucine--tRNA ligasePRO_0000098526Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi290434.BG0858.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65ZU1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q65ZU1-1 [UniParc]FASTAAdd to Basket

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    MFKKVENKAN FPKIEEKILK FWNDNKIFEK SMEQREGCEE FTFYDGPPFA     50
    TGLPHFGHFV PNTIKDIIPR YQTMKGKYVK RNFGWDTHGL PVEYEVEKKL 100
    GISGKYEIEN YGIENFNKEC KKIVLRYTEE WKNIILRLGR WVDFEKGYKT 150
    MDISFMESVW WVFKNLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK 200
    EVNDPSLTIK FKIKDKNEYL LAWTTTPWTL PSNLGIAVGK EIEYSKIFDK 250
    KKEEILILGS KKINSYFDDE NAYTIIEKFK GSQLQGIEYE PIFNYFLEQK 300
    DKGAFKVHTA DYITTDDGTG IVHIAPFGEE DYRVLKKHTN VDIIDPLDAE 350
    CKFTNRVKDF KGLFVKDADK KIIENLKLRN FLFKRENYLH RYPFCYRTNY 400
    PIIYRPISSW FVNVEKIKTQ LLEVNEKINW MPAHLKKGRF GKWLENAKDW 450
    AISRNRFWGN PIPIWICSKT GKKICVGSRK ELEELSGQKI EDLHKDKIDK 500
    ITWPSKDGGT FIRTSEVLDC WFESGAMPYA SNHYPFANES NFKNIFPADF 550
    IAEGLDQTRG WFYTLTILGT SLFENTAFKN VIVNGLVLSS DGRKMSKSFK 600
    NYTDPMEVIN TFGADALRLY LIMSPVVKAD DLKYSDNGVR DVLKNIIIPI 650
    WNAYSFFTTY AIIDKFKPTK NLSLVKSNNL DKWIISELES LKKILNKEID 700
    KYNLTKSIES LLEFIDKLNN WYIRRSRRRF WKSENDKDKN DAYETLYYAI 750
    KTLMILLAPF IPFITEEIYQ NLKTDEDKQS IHLNDYPKAN ENFIDKTIEE 800
    KINLARKITS MARSLRSLHN IKIRMPISTI YVVTKNQNEQ NMLIEMQEII 850
    LDEINVKEMK IKSNEEELIT YKAKANFKEL GKKLGKDMKT VSIEISKLKN 900
    EDIIKIINGI SHEIKVDNAK YYLSLNDIIL ERDEKDNLKV INEESITIGI 950
    DSLITQELYL EGLTREFVRQ IQNLRKEKNF DVSDRINLYI ENNATLEEIL 1000
    NKFEKYIKTE TLALNIILNK SKLEKKINLD NDIFTIIGIE KC 1042
    Length:1,042
    Mass (Da):122,408
    Last modified:October 11, 2004 - v1
    Checksum:i6389CBC417E852C4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000013 Genomic DNA. Translation: AAU07680.1.
    RefSeqiYP_073272.1. NC_006156.1.

    Genome annotation databases

    EnsemblBacteriaiAAU07680; AAU07680; BG0858.
    GeneIDi2957738.
    KEGGibga:BG0858.
    PATRICi20567293. VBIBorGar102262_0943.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000013 Genomic DNA. Translation: AAU07680.1 .
    RefSeqi YP_073272.1. NC_006156.1.

    3D structure databases

    ProteinModelPortali Q65ZU1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290434.BG0858.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU07680 ; AAU07680 ; BG0858 .
    GeneIDi 2957738.
    KEGGi bga:BG0858.
    PATRICi 20567293. VBIBorGar102262_0943.

    Phylogenomic databases

    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BGAR290434:BG0858-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PBi.

    Entry informationi

    Entry nameiSYI_BORGA
    AccessioniPrimary (citable) accession number: Q65ZU1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3