Alkaline serine protease - Pseudoalteromonas sp. AS-11

Preferred order of sections

Names and origin

Protein names

Submitted name:
Alkaline serine protease EMBL BAD51450.1

Gene names

Name:

apa1 EMBL BAD51450.1

Organism

Pseudoalteromonas sp. AS-11 EMBL BAD51450.1

Taxonomic identifier

247492 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	817 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 1V6C PDB 1WVM PDB 1Y9Z Magnesium PDB 1WVM Metal-binding PDB 1V6C PDB 1WVM PDB 1Y9Z
Molecular function	Hydrolase Protease EMBL BAD51450.1
Technical term	3D-structure PDB 1V6C PDB 1WVM PDB 1Y9Z
Gene Ontology (GO)
Biological process	negative regulation of catalytic activity Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	identical protein binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

146

1

Calcium 1 PDB 1V6C PDB 1WVM PDB 1Y9Z

Metal binding

183

1

Calcium 1 PDB 1V6C PDB 1WVM PDB 1Y9Z

Metal binding

220

1

Calcium 1; via carbonyl oxygen PDB 1V6C PDB 1WVM PDB 1Y9Z

Metal binding

222

1

Calcium 1 PDB 1V6C PDB 1WVM PDB 1Y9Z

Metal binding

224

1

Calcium 1; via carbonyl oxygen PDB 1V6C PDB 1WVM PDB 1Y9Z

Metal binding

226

1

Calcium 1; via carbonyl oxygen PDB 1V6C PDB 1WVM PDB 1Y9Z

Metal binding

313

1

Calcium 2; via carbonyl oxygen PDB 1V6C

Metal binding

313

1

Magnesium 1; via carbonyl oxygen PDB 1WVM

Metal binding

315

1

Calcium 2; via carbonyl oxygen PDB 1V6C

Metal binding

315

1

Magnesium 1; via carbonyl oxygen PDB 1WVM

Metal binding

318

1

Calcium 2; via carbonyl oxygen PDB 1V6C

Metal binding

318

1

Magnesium 1; via carbonyl oxygen PDB 1WVM

Metal binding

318

1

Magnesium 2; via carbonyl oxygen PDB 1WVM

Metal binding

320

1

Magnesium 2; via amide nitrogen PDB 1WVM

Metal binding

339

1

Calcium 2 PDB 1V6C

Metal binding

339

1

Magnesium 1 PDB 1WVM

Metal binding

339

1

Magnesium 2; via carbonyl oxygen PDB 1WVM

Metal binding

341

1

Magnesium 2 PDB 1WVM

Metal binding

548

1

Calcium 3 PDB 1V6C PDB 1WVM PDB 1Y9Z

Metal binding

549

1

Calcium 3; via carbonyl oxygen PDB 1V6C PDB 1WVM PDB 1Y9Z

Metal binding

551

1

Calcium 3; via carbonyl oxygen PDB 1V6C PDB 1WVM PDB 1Y9Z

Metal binding

553

1

Calcium 3; via carbonyl oxygen PDB 1V6C PDB 1WVM PDB 1Y9Z

Metal binding

555

1

Calcium 3 PDB 1V6C PDB 1WVM PDB 1Y9Z

Sequences

Sequence

Length

Mass (Da)

Tools

Q65Z69 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 83A11C17B1A39513
Show »

FASTA

817

85,145

        10         20         30         40         50         60 
MKKNKNSAVV LSMTALALAI STQVNAAPSP LQLYATNQQI DQQSPLPKRY IVKFKNDNAA 

        70         80         90        100        110        120 
QTLSSSSVTQ NVGVQPETNS MSYQPRSNEV FSQFRALNSV KAREMKRVGR SNSYSVKLDS 

       130        140        150        160        170        180 
PSIKALRLRQ DVEYVEEDLP RRLLAETTPW GQTFVGATVL SDSQAGNRTI CIIDSGYDRS 

       190        200        210        220        230        240 
HNDLNANNVT GTNNSGTGNW YQPGNNNAHG THVAGTIAAI ANNEGVVGVM PNQNANIHIV 

       250        260        270        280        290        300 
KVFNEAGWGY SSSLVAAIDT CVNSGGANVV TMSLGGSGST TTERNALNTH YNNGVLLIAA 

       310        320        330        340        350        360 
AGNAGDSSYS YPASYDSVMS VAAVDSNLDH AAFSQYTDQV EISGPGEAIL STVTVGEGRL 

       370        380        390        400        410        420 
ADITIGGQSY FSNGVVPHNR LTPSGTSYAP APINASATGA LAECTVNGTS FSCGNMANKI 

       430        440        450        460        470        480 
CLVERVGNQG SSYPEINSTK ACKTAGAKGI IVYSNSALPG LQNPFLVDAN SDITVPSVSV 

       490        500        510        520        530        540 
DRATGLALKA KLGQSTTVSN QGNQDYEYYN GTSMATPHVS GVATLVWSYH PECSASQVRA 

       550        560        570        580        590        600 
ALNATADDLS VAGRDNQTGY GMINAVAAKA YLDESCTGPT DPGTGPGTGD SVLVKGVAKT 

       610        620        630        640        650        660 
GLAGAKSDEL YFSLDVPAGA KDLTFTMNGG TGDADLYVQY GASPTSSSYD CRPWKSGNAE 

       670        680        690        700        710        720 
SCPIATPQTG TYYVMVQGYS AFSNVNLMAN YTAATTSGGN TGGPASYTNT GNYTIPDNNT 

       730        740        750        760        770        780 
TGITSPITVS RTGDSGSVSV SIGIIHTYIG DLKIQLVSPT GQTVVLHNNT GGGTDNLTQT 

       790        800        810 
YTANMAGVES SGVWTLKAVD NAKQDTGYID TWSLSFQ

« Hide

References

[1]	"Subtilisin-like Protease Apa1 from Antarctic Psychrotroph Pseudoalteromonas sp. Strain AS-11." Dong D., Aise K., Koizumi T., Watanabe K. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: AS-11 EMBL BAD51450.1.
[2]	"Crystal Structure of Psychrophilic Subtilisin-like Serine Protease from Antarctic Psychrotroph Pseudoalteromonas sp. AS-11 at 0.14 nm resolution." Dong D., Watanabe K. Submitted (DEC-2004) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 145-585 IN COMPLEX WITH CALCIUM.
[3]	"Crystal Structure of Psychrophilic Subtilisin-like Serine Protease from Antarctic Psychrotroph Pseudoalteromonas sp. AS-11 at 0.16 nm resolution." Dong D., Watanabe K. Submitted (DEC-2004) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 145-585 IN COMPLEX WITH CALCIUM AND MAGNESIUM.
[4]	"Crystal Structure of Psychrophilic Subtilisin-like Protease Apa1 from Antarctic Psychrotroph Pseudoalteromonas sp. AS-11." Dong D., Ihara T., Motoshima H., Watanabe K. Submitted (NOV-2003) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 145-585 IN COMPLEX WITH CALCIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB120714 Genomic DNA. Translation: BAD51450.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V6C	X-ray	1.80	A/B	145-585	[»]
1WVM	X-ray	1.60	A/B	145-585	[»]
1Y9Z	X-ray	1.40	A/B	145-585	[»]

ProteinModelPortal

Q65Z69.

SMR

Q65Z69. Positions 145-580.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008979. Galactose-bd-like.
IPR007280. Peptidase_C_arc/bac.
IPR000209. Peptidase_S8/S53.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR010259. Prot_inh_S8A.
IPR003137. Protease-assoc_domain.
IPR002884. PrprotnconvertsP.
[Graphical view]

Gene3D

G3DSA:3.40.50.200. Pept_S8_S53. 2 hits.

PANTHER

PTHR10795. SubtilSerProt. 1 hit.

Pfam

PF05922. Inhibitor_I9. 1 hit.
PF01483. P_proprotein. 1 hit.
PF02225. PA. 1 hit.
PF00082. Peptidase_S8. 2 hits.
PF04151. PPC. 1 hit.
[Graphical view]

PRINTS

PR00723. SUBTILISIN.

SUPFAM

SSF49785. Gal_bind_like. 1 hit.
SSF52743. Pept_S8_S53. 1 hit.
SSF54897. Prot_inh_propept. 1 hit.

PROSITE

PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q65Z69_9GAMM

Accession

Primary (citable) accession number: Q65Z69

Entry history

Integrated into UniProtKB/TrEMBL:	October 25, 2004
Last sequence update:	October 25, 2004
Last modified:	November 16, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)