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Q65Z40 (WAPL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Wings apart-like protein homolog
Alternative name(s):
Dioxin-inducible factor 2
Short name=DIF-2
Gene names
Name:Wapal
Synonyms:Kiaa0261, Wapl
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1200 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair By similarity.

Subunit structure

Interacts with the cohesin complex throughout the cell cycle; interacts with both chromatin-bound and soluble pools of the complex. Interacts with RAD21; the interaction is direct By similarity. Interacts with PDS5A; the interaction is direct, cohesin-dependent and competitive with CDCA5/SORORIN By similarity. Interacts (via FGF motifs) with PDS5B; the interaction is direct By similarity. Interacts with a SMC1 protein (SMC1A or SMC1B) and SMC3 By similarity. Ref.5

Subcellular location

Nucleus By similarity. Chromosome By similarity. Cytoplasm By similarity. Note: Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase By similarity.

Tissue specificity

Highly expressed in large pachytene spermatocytes of testis. Down-regulated by dioxin in testis. Ref.1

Sequence similarities

Belongs to the WAPL family.

Contains 1 WAPL domain.

Sequence caution

The sequence BAC97915.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentChromosome
Cytoplasm
Nucleus
   DomainCoiled coil
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmitotic nuclear division

Inferred from mutant phenotype PubMed 23975099. Source: MGI

negative regulation of DNA replication

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of chromatin binding

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of sister chromatid cohesion

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of fibroblast proliferation

Inferred from mutant phenotype PubMed 23975099. Source: MGI

protein localization to chromatin

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of chromosome condensation

Inferred from mutant phenotype PubMed 23975099. Source: MGI

regulation of chromosome segregation

Inferred from mutant phenotype PubMed 23975099. Source: MGI

regulation of cohesin localization to chromatin

Inferred from sequence or structural similarity. Source: UniProtKB

response to toxic substance

Inferred from direct assay Ref.1. Source: MGI

   Cellular_componentchromatin

Inferred from sequence or structural similarity. Source: UniProtKB

cohesin complex

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

microtubule cytoskeleton

Inferred from electronic annotation. Source: Ensembl

synaptonemal complex

Inferred from direct assay Ref.1. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12001200Wings apart-like protein homolog
PRO_0000245233

Regions

Domain635 – 1179545WAPL
Region1 – 668668Mediates interaction with the cohesin complex By similarity
Coiled coil266 – 29328 Potential
Coiled coil751 – 79141 Potential
Motif73 – 753FGF motif 1
Motif437 – 4393FGF motif 2
Motif462 – 4643FGF motif 3

Amino acid modifications

Modified residue771Phosphoserine Ref.4 Ref.7
Modified residue1681N6-acetyllysine By similarity
Modified residue2261Phosphoserine By similarity
Modified residue2281Phosphoserine By similarity
Modified residue3881Phosphoserine By similarity
Modified residue4681Phosphoserine Ref.6
Modified residue4701Phosphoserine Ref.6
Modified residue9141Phosphoserine By similarity

Experimental info

Sequence conflict7571L → V in BAD44717. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q65Z40 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 7697FA31AABBC573

FASTA1,200134,070
        10         20         30         40         50         60 
MTSRFGKTYS RKGGNGSSKF DEVFSNKRTT LSTKWGETTF MAKLGQKRPN FKPDIQEIPK 

        70         80         90        100        110        120 
KPKVEEEDTG DPFGFDSDDE SLPVSSKNLA QGKGSSYSES SEAAQLEEVT SVFEANSKCS 

       130        140        150        160        170        180 
HVVGEDSFAS DRCLLVEDTL IGKEKSISRI PEDNANKSSC TKLLTSDKVE NFSEEHEKNS 

       190        200        210        220        230        240 
HHFHKNAEDS TKKPNAETAV ASEYKADETK ETNDTWNSQS GKRTESPSES CPVKGSVRTG 

       250        260        270        280        290        300 
LYEWDNDFED IRSEDCILSL DNESLLEMKD EDLKNRIGGL ENLNETFEED IIQSVLRPSN 

       310        320        330        340        350        360 
CRTYCRANKA RSSQGASNFD KLMDGTSQSL AKANSESSKD GLNQAKKGSA SCGTSFRGTV 

       370        380        390        400        410        420 
GRTRDYTVLH PSCLSVCNVT IQDTMERSMD EFTASTPADL GEAGRLRKKA DIATSKTTTR 

       430        440        450        460        470        480 
FRPSNTKSKK DVKLEFFGFE DHDETGGDEG GSGSSNYKIK YFGFDDLSES EDDDDDDCQV 

       490        500        510        520        530        540 
ERKKDKKRTK TAPSPSQQPP PESSDNSQDS QSSTNNAENL DFTEDLPGVP ESVKKPISKQ 

       550        560        570        580        590        600 
GDKSKENTRK IFSGPKRSPT KAVYNARHWN HPDSEELPGP PIAKPQRVTV RLSSKEPNQK 

       610        620        630        640        650        660 
DDGVFKAPAP PLKVIKTVTI PTQPYQEIVT ALKCRKEDKE LYTVVQHVKH FNDVVEFGEN 

       670        680        690        700        710        720 
QEFTDDIEYL LSGLKSTQPL NTRCLSVISL ATKCAMPSFR MHLRAHGMVA MVFKTLDDSQ 

       730        740        750        760        770        780 
HHQNLSLCTA ALMYILSRDR LNMDLDRASL DLMIRLLELE QDASSAKLLN EKDMNKIKEK 

       790        800        810        820        830        840 
IRRLCETVHN KHLDLENITT GHLAMETLLS LTSKRAGDWF KEELRLLGGL DHIVDKVKEC 

       850        860        870        880        890        900 
VDHLSRDDED EEKLVASLWG AERCLRVLES VTVHNPENQS YLIAYKDSQL IISSAKALQH 

       910        920        930        940        950        960 
CEDLIQQYNR AENSICVADS NPLPYQNVTN HVGKAVEDCM RAIIGVLLNL TNDNEWGSTK 

       970        980        990       1000       1010       1020 
TGEQEGLIGT AMNCVLQVPK YLPQEQRFDI RVLGLGLLIN LVEYSARNRH CLVNMQTSCS 

      1030       1040       1050       1060       1070       1080 
FDSSFSSGEG DHSLRLAGQV HAVQALVQLF LERERAAQLA ESKTDELIKD APTTQHDKSG 

      1090       1100       1110       1120       1130       1140 
EWQETSGEIQ WVSTEKTDGA EEKQKKEEED EELDLNKALQ HAGKHMEDCI VASYTALLLG 

      1150       1160       1170       1180       1190       1200 
CLCQESPINV TTVREYLPEG DFSIMTEMLK KFLSFMNLTC AVGTTGQKSI SRVIEYLEHC 

« Hide

References

« Hide 'large scale' references
[1]"A dioxin sensitive gene, mammalian WAPL, is implicated in spermatogenesis."
Kuroda M., Oikawa K., Ohbayashi T., Yoshida K., Yamada K., Mimura J., Matsuda Y., Fujii-Kuriyama Y., Mukai K.
FEBS Lett. 579:167-172(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryonic tail.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[5]"Wapl controls the dynamic association of cohesin with chromatin."
Kueng S., Hegemann B., Peters B.H., Lipp J.J., Schleiffer A., Mechtler K., Peters J.M.
Cell 127:955-967(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH THE COHESIN COMPLEX.
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468 AND SER-470, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[7]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB167349 mRNA. Translation: BAD44717.1.
AK129105 mRNA. Translation: BAC97915.1. Different initiation.
BC139434 mRNA. Translation: AAI39435.1.
CCDSCCDS36880.1.
RefSeqNP_001004436.2. NM_001004436.3.
UniGeneMm.27957.

3D structure databases

ProteinModelPortalQ65Z40.
SMRQ65Z40. Positions 640-1200.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid230082. 5 interactions.
IntActQ65Z40. 1 interaction.

PTM databases

PhosphoSiteQ65Z40.

Proteomic databases

MaxQBQ65Z40.
PaxDbQ65Z40.
PRIDEQ65Z40.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000048263; ENSMUSP00000040232; ENSMUSG00000041408.
ENSMUST00000169910; ENSMUSP00000130547; ENSMUSG00000041408.
GeneID218914.
KEGGmmu:218914.
UCSCuc007tbi.1. mouse.

Organism-specific databases

CTD23063.
MGIMGI:2675859. Wapal.
RougeSearch...

Phylogenomic databases

eggNOGNOG297597.
GeneTreeENSGT00390000015768.
HOGENOMHOG000001563.
HOVERGENHBG088338.
InParanoidQ65Z40.
OMAWNSQFGK.
OrthoDBEOG7DZ8J3.
TreeFamTF323477.

Gene expression databases

ArrayExpressQ65Z40.
BgeeQ65Z40.
CleanExMM_WAPAL.
GenevestigatorQ65Z40.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR012502. WAPL_metazoan_plants.
IPR022771. WAPL_prot.
[Graphical view]
PfamPF07814. WAPL. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS51271. WAPL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio376486.
PROQ65Z40.
SOURCESearch...

Entry information

Entry nameWAPL_MOUSE
AccessionPrimary (citable) accession number: Q65Z40
Secondary accession number(s): B9EIG8, Q6ZQE9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot