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Q65Z40

- WAPL_MOUSE

UniProt

Q65Z40 - WAPL_MOUSE

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Protein

Wings apart-like protein homolog

Gene

Wapal

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair (By similarity).By similarity

GO - Biological processi

  1. mitotic nuclear division Source: MGI
  2. negative regulation of chromatin binding Source: UniProtKB
  3. negative regulation of DNA replication Source: UniProtKB
  4. negative regulation of sister chromatid cohesion Source: UniProtKB
  5. positive regulation of fibroblast proliferation Source: MGI
  6. protein localization to chromatin Source: UniProtKB
  7. regulation of chromosome condensation Source: MGI
  8. regulation of chromosome segregation Source: MGI
  9. regulation of cohesin localization to chromatin Source: UniProtKB
  10. response to toxic substance Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_196614. Establishment of Sister Chromatid Cohesion.
REACT_196634. Cohesin Loading onto Chromatin.
REACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.

Names & Taxonomyi

Protein namesi
Recommended name:
Wings apart-like protein homolog
Alternative name(s):
Dioxin-inducible factor 2
Short name:
DIF-2
Gene namesi
Name:Wapal
Synonyms:Kiaa0261, Wapl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:2675859. Wapal.

Subcellular locationi

Nucleus By similarity. Chromosome By similarity. Cytoplasm By similarity
Note: Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase (By similarity).By similarity

GO - Cellular componenti

  1. chromatin Source: UniProtKB
  2. cohesin complex Source: UniProtKB
  3. cytoplasm Source: UniProtKB
  4. microtubule cytoskeleton Source: Ensembl
  5. synaptonemal complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12001200Wings apart-like protein homologPRO_0000245233Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei77 – 771Phosphoserine2 Publications
Modified residuei168 – 1681N6-acetyllysineBy similarity
Modified residuei226 – 2261PhosphoserineBy similarity
Modified residuei228 – 2281PhosphoserineBy similarity
Modified residuei388 – 3881PhosphoserineBy similarity
Modified residuei468 – 4681Phosphoserine1 Publication
Modified residuei470 – 4701Phosphoserine1 Publication
Modified residuei914 – 9141PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ65Z40.
PaxDbiQ65Z40.
PRIDEiQ65Z40.

PTM databases

PhosphoSiteiQ65Z40.

Expressioni

Tissue specificityi

Highly expressed in large pachytene spermatocytes of testis. Down-regulated by dioxin in testis.1 Publication

Gene expression databases

BgeeiQ65Z40.
CleanExiMM_WAPAL.
ExpressionAtlasiQ65Z40. baseline and differential.
GenevestigatoriQ65Z40.

Interactioni

Subunit structurei

Interacts with the cohesin complex throughout the cell cycle; interacts with both chromatin-bound and soluble pools of the complex. Interacts with RAD21; the interaction is direct (By similarity). Interacts with PDS5A; the interaction is direct, cohesin-dependent and competitive with CDCA5/SORORIN (By similarity). Interacts (via FGF motifs) with PDS5B; the interaction is direct (By similarity). Interacts with a SMC1 protein (SMC1A or SMC1B) and SMC3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi230082. 5 interactions.
IntActiQ65Z40. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ65Z40.
SMRiQ65Z40. Positions 640-1200.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini635 – 1179545WAPLPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 668668Mediates interaction with the cohesin complexBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili266 – 29328Sequence AnalysisAdd
BLAST
Coiled coili751 – 79141Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi73 – 753FGF motif 1
Motifi437 – 4393FGF motif 2
Motifi462 – 4643FGF motif 3

Sequence similaritiesi

Belongs to the WAPL family.Curated
Contains 1 WAPL domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG297597.
GeneTreeiENSGT00390000015768.
HOGENOMiHOG000001563.
HOVERGENiHBG088338.
InParanoidiQ65Z40.
OMAiWNSQFGK.
OrthoDBiEOG7DZ8J3.
TreeFamiTF323477.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR012502. WAPL_metazoan_plants.
IPR022771. WAPL_prot.
[Graphical view]
PfamiPF07814. WAPL. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51271. WAPL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65Z40-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSRFGKTYS RKGGNGSSKF DEVFSNKRTT LSTKWGETTF MAKLGQKRPN
60 70 80 90 100
FKPDIQEIPK KPKVEEEDTG DPFGFDSDDE SLPVSSKNLA QGKGSSYSES
110 120 130 140 150
SEAAQLEEVT SVFEANSKCS HVVGEDSFAS DRCLLVEDTL IGKEKSISRI
160 170 180 190 200
PEDNANKSSC TKLLTSDKVE NFSEEHEKNS HHFHKNAEDS TKKPNAETAV
210 220 230 240 250
ASEYKADETK ETNDTWNSQS GKRTESPSES CPVKGSVRTG LYEWDNDFED
260 270 280 290 300
IRSEDCILSL DNESLLEMKD EDLKNRIGGL ENLNETFEED IIQSVLRPSN
310 320 330 340 350
CRTYCRANKA RSSQGASNFD KLMDGTSQSL AKANSESSKD GLNQAKKGSA
360 370 380 390 400
SCGTSFRGTV GRTRDYTVLH PSCLSVCNVT IQDTMERSMD EFTASTPADL
410 420 430 440 450
GEAGRLRKKA DIATSKTTTR FRPSNTKSKK DVKLEFFGFE DHDETGGDEG
460 470 480 490 500
GSGSSNYKIK YFGFDDLSES EDDDDDDCQV ERKKDKKRTK TAPSPSQQPP
510 520 530 540 550
PESSDNSQDS QSSTNNAENL DFTEDLPGVP ESVKKPISKQ GDKSKENTRK
560 570 580 590 600
IFSGPKRSPT KAVYNARHWN HPDSEELPGP PIAKPQRVTV RLSSKEPNQK
610 620 630 640 650
DDGVFKAPAP PLKVIKTVTI PTQPYQEIVT ALKCRKEDKE LYTVVQHVKH
660 670 680 690 700
FNDVVEFGEN QEFTDDIEYL LSGLKSTQPL NTRCLSVISL ATKCAMPSFR
710 720 730 740 750
MHLRAHGMVA MVFKTLDDSQ HHQNLSLCTA ALMYILSRDR LNMDLDRASL
760 770 780 790 800
DLMIRLLELE QDASSAKLLN EKDMNKIKEK IRRLCETVHN KHLDLENITT
810 820 830 840 850
GHLAMETLLS LTSKRAGDWF KEELRLLGGL DHIVDKVKEC VDHLSRDDED
860 870 880 890 900
EEKLVASLWG AERCLRVLES VTVHNPENQS YLIAYKDSQL IISSAKALQH
910 920 930 940 950
CEDLIQQYNR AENSICVADS NPLPYQNVTN HVGKAVEDCM RAIIGVLLNL
960 970 980 990 1000
TNDNEWGSTK TGEQEGLIGT AMNCVLQVPK YLPQEQRFDI RVLGLGLLIN
1010 1020 1030 1040 1050
LVEYSARNRH CLVNMQTSCS FDSSFSSGEG DHSLRLAGQV HAVQALVQLF
1060 1070 1080 1090 1100
LERERAAQLA ESKTDELIKD APTTQHDKSG EWQETSGEIQ WVSTEKTDGA
1110 1120 1130 1140 1150
EEKQKKEEED EELDLNKALQ HAGKHMEDCI VASYTALLLG CLCQESPINV
1160 1170 1180 1190 1200
TTVREYLPEG DFSIMTEMLK KFLSFMNLTC AVGTTGQKSI SRVIEYLEHC
Length:1,200
Mass (Da):134,070
Last modified:July 27, 2011 - v2
Checksum:i7697FA31AABBC573
GO

Sequence cautioni

The sequence BAC97915.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti757 – 7571L → V in BAD44717. (PubMed:15620708)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB167349 mRNA. Translation: BAD44717.1.
AK129105 mRNA. Translation: BAC97915.1. Different initiation.
BC139434 mRNA. Translation: AAI39435.1.
CCDSiCCDS36880.1.
RefSeqiNP_001004436.2. NM_001004436.4.
NP_001288259.1. NM_001301330.1.
UniGeneiMm.27957.

Genome annotation databases

EnsembliENSMUST00000048263; ENSMUSP00000040232; ENSMUSG00000041408.
ENSMUST00000169910; ENSMUSP00000130547; ENSMUSG00000041408.
GeneIDi218914.
KEGGimmu:218914.
UCSCiuc007tbi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB167349 mRNA. Translation: BAD44717.1 .
AK129105 mRNA. Translation: BAC97915.1 . Different initiation.
BC139434 mRNA. Translation: AAI39435.1 .
CCDSi CCDS36880.1.
RefSeqi NP_001004436.2. NM_001004436.4.
NP_001288259.1. NM_001301330.1.
UniGenei Mm.27957.

3D structure databases

ProteinModelPortali Q65Z40.
SMRi Q65Z40. Positions 640-1200.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 230082. 5 interactions.
IntActi Q65Z40. 1 interaction.

PTM databases

PhosphoSitei Q65Z40.

Proteomic databases

MaxQBi Q65Z40.
PaxDbi Q65Z40.
PRIDEi Q65Z40.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000048263 ; ENSMUSP00000040232 ; ENSMUSG00000041408 .
ENSMUST00000169910 ; ENSMUSP00000130547 ; ENSMUSG00000041408 .
GeneIDi 218914.
KEGGi mmu:218914.
UCSCi uc007tbi.1. mouse.

Organism-specific databases

CTDi 23063.
MGIi MGI:2675859. Wapal.
Rougei Search...

Phylogenomic databases

eggNOGi NOG297597.
GeneTreei ENSGT00390000015768.
HOGENOMi HOG000001563.
HOVERGENi HBG088338.
InParanoidi Q65Z40.
OMAi WNSQFGK.
OrthoDBi EOG7DZ8J3.
TreeFami TF323477.

Enzyme and pathway databases

Reactomei REACT_196614. Establishment of Sister Chromatid Cohesion.
REACT_196634. Cohesin Loading onto Chromatin.
REACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.

Miscellaneous databases

NextBioi 376486.
PROi Q65Z40.
SOURCEi Search...

Gene expression databases

Bgeei Q65Z40.
CleanExi MM_WAPAL.
ExpressionAtlasi Q65Z40. baseline and differential.
Genevestigatori Q65Z40.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR012502. WAPL_metazoan_plants.
IPR022771. WAPL_prot.
[Graphical view ]
Pfami PF07814. WAPL. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS51271. WAPL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A dioxin sensitive gene, mammalian WAPL, is implicated in spermatogenesis."
    Kuroda M., Oikawa K., Ohbayashi T., Yoshida K., Yamada K., Mimura J., Matsuda Y., Fujii-Kuriyama Y., Mukai K.
    FEBS Lett. 579:167-172(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryonic tail.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  5. "Wapl controls the dynamic association of cohesin with chromatin."
    Kueng S., Hegemann B., Peters B.H., Lipp J.J., Schleiffer A., Mechtler K., Peters J.M.
    Cell 127:955-967(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH THE COHESIN COMPLEX.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468 AND SER-470, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiWAPL_MOUSE
AccessioniPrimary (citable) accession number: Q65Z40
Secondary accession number(s): B9EIG8, Q6ZQE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3