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Q65Z40

- WAPL_MOUSE

UniProt

Q65Z40 - WAPL_MOUSE

Protein

Wings apart-like protein homolog

Gene

Wapal

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair By similarity.By similarity

    GO - Biological processi

    1. mitotic nuclear division Source: MGI
    2. negative regulation of chromatin binding Source: UniProtKB
    3. negative regulation of DNA replication Source: UniProtKB
    4. negative regulation of sister chromatid cohesion Source: UniProtKB
    5. positive regulation of fibroblast proliferation Source: MGI
    6. protein localization to chromatin Source: UniProtKB
    7. regulation of chromosome condensation Source: MGI
    8. regulation of chromosome segregation Source: MGI
    9. regulation of cohesin localization to chromatin Source: UniProtKB
    10. response to toxic substance Source: MGI

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_196614. Establishment of Sister Chromatid Cohesion.
    REACT_196634. Cohesin Loading onto Chromatin.
    REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Wings apart-like protein homolog
    Alternative name(s):
    Dioxin-inducible factor 2
    Short name:
    DIF-2
    Gene namesi
    Name:Wapal
    Synonyms:Kiaa0261, Wapl
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:2675859. Wapal.

    Subcellular locationi

    Nucleus By similarity. Chromosome By similarity. Cytoplasm By similarity
    Note: Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase By similarity.By similarity

    GO - Cellular componenti

    1. chromatin Source: UniProtKB
    2. cohesin complex Source: UniProtKB
    3. cytoplasm Source: UniProtKB
    4. microtubule cytoskeleton Source: Ensembl
    5. synaptonemal complex Source: MGI

    Keywords - Cellular componenti

    Chromosome, Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12001200Wings apart-like protein homologPRO_0000245233Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei77 – 771Phosphoserine2 Publications
    Modified residuei168 – 1681N6-acetyllysineBy similarity
    Modified residuei226 – 2261PhosphoserineBy similarity
    Modified residuei228 – 2281PhosphoserineBy similarity
    Modified residuei388 – 3881PhosphoserineBy similarity
    Modified residuei468 – 4681Phosphoserine1 Publication
    Modified residuei470 – 4701Phosphoserine1 Publication
    Modified residuei914 – 9141PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ65Z40.
    PaxDbiQ65Z40.
    PRIDEiQ65Z40.

    PTM databases

    PhosphoSiteiQ65Z40.

    Expressioni

    Tissue specificityi

    Highly expressed in large pachytene spermatocytes of testis. Down-regulated by dioxin in testis.1 Publication

    Gene expression databases

    ArrayExpressiQ65Z40.
    BgeeiQ65Z40.
    CleanExiMM_WAPAL.
    GenevestigatoriQ65Z40.

    Interactioni

    Subunit structurei

    Interacts with the cohesin complex throughout the cell cycle; interacts with both chromatin-bound and soluble pools of the complex. Interacts with RAD21; the interaction is direct By similarity. Interacts with PDS5A; the interaction is direct, cohesin-dependent and competitive with CDCA5/SORORIN By similarity. Interacts (via FGF motifs) with PDS5B; the interaction is direct By similarity. Interacts with a SMC1 protein (SMC1A or SMC1B) and SMC3 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi230082. 5 interactions.
    IntActiQ65Z40. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65Z40.
    SMRiQ65Z40. Positions 640-1200.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini635 – 1179545WAPLPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 668668Mediates interaction with the cohesin complexBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili266 – 29328Sequence AnalysisAdd
    BLAST
    Coiled coili751 – 79141Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi73 – 753FGF motif 1
    Motifi437 – 4393FGF motif 2
    Motifi462 – 4643FGF motif 3

    Sequence similaritiesi

    Belongs to the WAPL family.Curated
    Contains 1 WAPL domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG297597.
    GeneTreeiENSGT00390000015768.
    HOGENOMiHOG000001563.
    HOVERGENiHBG088338.
    InParanoidiQ65Z40.
    OMAiWNSQFGK.
    OrthoDBiEOG7DZ8J3.
    TreeFamiTF323477.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    IPR012502. WAPL_metazoan_plants.
    IPR022771. WAPL_prot.
    [Graphical view]
    PfamiPF07814. WAPL. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS51271. WAPL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q65Z40-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSRFGKTYS RKGGNGSSKF DEVFSNKRTT LSTKWGETTF MAKLGQKRPN     50
    FKPDIQEIPK KPKVEEEDTG DPFGFDSDDE SLPVSSKNLA QGKGSSYSES 100
    SEAAQLEEVT SVFEANSKCS HVVGEDSFAS DRCLLVEDTL IGKEKSISRI 150
    PEDNANKSSC TKLLTSDKVE NFSEEHEKNS HHFHKNAEDS TKKPNAETAV 200
    ASEYKADETK ETNDTWNSQS GKRTESPSES CPVKGSVRTG LYEWDNDFED 250
    IRSEDCILSL DNESLLEMKD EDLKNRIGGL ENLNETFEED IIQSVLRPSN 300
    CRTYCRANKA RSSQGASNFD KLMDGTSQSL AKANSESSKD GLNQAKKGSA 350
    SCGTSFRGTV GRTRDYTVLH PSCLSVCNVT IQDTMERSMD EFTASTPADL 400
    GEAGRLRKKA DIATSKTTTR FRPSNTKSKK DVKLEFFGFE DHDETGGDEG 450
    GSGSSNYKIK YFGFDDLSES EDDDDDDCQV ERKKDKKRTK TAPSPSQQPP 500
    PESSDNSQDS QSSTNNAENL DFTEDLPGVP ESVKKPISKQ GDKSKENTRK 550
    IFSGPKRSPT KAVYNARHWN HPDSEELPGP PIAKPQRVTV RLSSKEPNQK 600
    DDGVFKAPAP PLKVIKTVTI PTQPYQEIVT ALKCRKEDKE LYTVVQHVKH 650
    FNDVVEFGEN QEFTDDIEYL LSGLKSTQPL NTRCLSVISL ATKCAMPSFR 700
    MHLRAHGMVA MVFKTLDDSQ HHQNLSLCTA ALMYILSRDR LNMDLDRASL 750
    DLMIRLLELE QDASSAKLLN EKDMNKIKEK IRRLCETVHN KHLDLENITT 800
    GHLAMETLLS LTSKRAGDWF KEELRLLGGL DHIVDKVKEC VDHLSRDDED 850
    EEKLVASLWG AERCLRVLES VTVHNPENQS YLIAYKDSQL IISSAKALQH 900
    CEDLIQQYNR AENSICVADS NPLPYQNVTN HVGKAVEDCM RAIIGVLLNL 950
    TNDNEWGSTK TGEQEGLIGT AMNCVLQVPK YLPQEQRFDI RVLGLGLLIN 1000
    LVEYSARNRH CLVNMQTSCS FDSSFSSGEG DHSLRLAGQV HAVQALVQLF 1050
    LERERAAQLA ESKTDELIKD APTTQHDKSG EWQETSGEIQ WVSTEKTDGA 1100
    EEKQKKEEED EELDLNKALQ HAGKHMEDCI VASYTALLLG CLCQESPINV 1150
    TTVREYLPEG DFSIMTEMLK KFLSFMNLTC AVGTTGQKSI SRVIEYLEHC 1200
    Length:1,200
    Mass (Da):134,070
    Last modified:July 27, 2011 - v2
    Checksum:i7697FA31AABBC573
    GO

    Sequence cautioni

    The sequence BAC97915.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti757 – 7571L → V in BAD44717. (PubMed:15620708)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB167349 mRNA. Translation: BAD44717.1.
    AK129105 mRNA. Translation: BAC97915.1. Different initiation.
    BC139434 mRNA. Translation: AAI39435.1.
    CCDSiCCDS36880.1.
    RefSeqiNP_001004436.2. NM_001004436.3.
    UniGeneiMm.27957.

    Genome annotation databases

    EnsembliENSMUST00000048263; ENSMUSP00000040232; ENSMUSG00000041408.
    ENSMUST00000169910; ENSMUSP00000130547; ENSMUSG00000041408.
    GeneIDi218914.
    KEGGimmu:218914.
    UCSCiuc007tbi.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB167349 mRNA. Translation: BAD44717.1 .
    AK129105 mRNA. Translation: BAC97915.1 . Different initiation.
    BC139434 mRNA. Translation: AAI39435.1 .
    CCDSi CCDS36880.1.
    RefSeqi NP_001004436.2. NM_001004436.3.
    UniGenei Mm.27957.

    3D structure databases

    ProteinModelPortali Q65Z40.
    SMRi Q65Z40. Positions 640-1200.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230082. 5 interactions.
    IntActi Q65Z40. 1 interaction.

    PTM databases

    PhosphoSitei Q65Z40.

    Proteomic databases

    MaxQBi Q65Z40.
    PaxDbi Q65Z40.
    PRIDEi Q65Z40.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000048263 ; ENSMUSP00000040232 ; ENSMUSG00000041408 .
    ENSMUST00000169910 ; ENSMUSP00000130547 ; ENSMUSG00000041408 .
    GeneIDi 218914.
    KEGGi mmu:218914.
    UCSCi uc007tbi.1. mouse.

    Organism-specific databases

    CTDi 23063.
    MGIi MGI:2675859. Wapal.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG297597.
    GeneTreei ENSGT00390000015768.
    HOGENOMi HOG000001563.
    HOVERGENi HBG088338.
    InParanoidi Q65Z40.
    OMAi WNSQFGK.
    OrthoDBi EOG7DZ8J3.
    TreeFami TF323477.

    Enzyme and pathway databases

    Reactomei REACT_196614. Establishment of Sister Chromatid Cohesion.
    REACT_196634. Cohesin Loading onto Chromatin.
    REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Miscellaneous databases

    NextBioi 376486.
    PROi Q65Z40.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q65Z40.
    Bgeei Q65Z40.
    CleanExi MM_WAPAL.
    Genevestigatori Q65Z40.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    IPR012502. WAPL_metazoan_plants.
    IPR022771. WAPL_prot.
    [Graphical view ]
    Pfami PF07814. WAPL. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS51271. WAPL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A dioxin sensitive gene, mammalian WAPL, is implicated in spermatogenesis."
      Kuroda M., Oikawa K., Ohbayashi T., Yoshida K., Yamada K., Mimura J., Matsuda Y., Fujii-Kuriyama Y., Mukai K.
      FEBS Lett. 579:167-172(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryonic tail.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    5. "Wapl controls the dynamic association of cohesin with chromatin."
      Kueng S., Hegemann B., Peters B.H., Lipp J.J., Schleiffer A., Mechtler K., Peters J.M.
      Cell 127:955-967(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH THE COHESIN COMPLEX.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468 AND SER-470, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiWAPL_MOUSE
    AccessioniPrimary (citable) accession number: Q65Z40
    Secondary accession number(s): B9EIG8, Q6ZQE9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 73 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3