Q65X70 (GPDH3_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 3, cytosolic
EC=1.1.1.8

Gene names

Ordered Locus Names:	Os05g0495700, LOC_Os05g41590
ORF Names:	OJ1579_G03.13, OsJ_19053

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in cell redox homeostasis By similarity.
Catalytic activity	sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.
Subcellular location	Cytoplasm › cytosol By similarity.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.
Sequence caution	The sequence BAF17847.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase [NAD+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 3, cytosolic		PRO_0000420179
Regions
	Nucleotide binding	44 – 49	6	NAD By similarity
	Region	343 – 344	2	Substrate binding By similarity
Sites
	Active site	281	1	Proton acceptor By similarity
	Binding site	192	1	NAD; via amide nitrogen By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	231	1	NAD; via amide nitrogen By similarity
	Binding site	343	1	NAD By similarity
	Binding site	371	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65X70 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: A47AC2476C4472F8	FASTA	465	51,485
	10 20 30 40 50 60 MVGSYAAGGG RGAAVAAVAE GKLDELRRRM GKADGDLLRI VGVGGGAWGS AFCALLQDAY 70 80 90 100 110 120 GRHRDKAQVR VWRRPGRAVD RATAEHLFEV INSREDVLRR LIRRCAYLKY VEARLGDRTL 130 140 150 160 170 180 YADEILRDGF CLNMVDTPLC PLKVVTNLQE AVWDADIVIN GLPSTETREV FGEIGRYWKE 190 200 210 220 230 240 RIRPPVIISL AKGIEASIDP VPRIITPTQM ISNATGVPLE NILYLGGPNI ASEIYNKEYA 250 260 270 280 290 300 NARICGADKW RKPLAKFLRQ PHFIVWDNSD LITHEVMGGL KNIYAIGAGM VAALTNESAT 310 320 330 340 350 360 SKSVYFSLCT SEMIYITHLL AEDPEKLAGP LLADTYVTLL KGRNAWYGQK LAKGELTLEM 370 380 390 400 410 420 GDSIKGKGTI QGVSAVHAFY ELLSQSSLSV THPEVKKLVA PVELCPILKT LYKILIKREL 430 440 450 460 ATDSILQAIR DESMYDPRER IEMSQRQCLY RPSLLGLPKV DITQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC112160 Genomic DNA. Translation: AAU44049.1. AP008211 Genomic DNA. Translation: BAF17847.1. Different initiation. CM000142 Genomic DNA. Translation: EEE64220.1. AK101484 mRNA. Translation: BAG95083.1.
RefSeq	NP_001055933.1. NM_001062468.1.
3D structure databases
ProteinModelPortal	Q65X70.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os05g41590.1; LOC_Os05g41590.1; LOC_Os05g41590.
GeneID	4339200.
KEGG	osa:4339200.
Organism-specific databases
Gramene	Q65X70.
Phylogenomic databases
OMA	YITHLLA.
ProtClustDB	CLSN2683781.
Gene expression databases
ArrayExpress	Q65X70.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR006168. G3P_DH_NAD-dep. IPR006109. G3P_DH_NAD-dep_C. IPR011128. G3P_DH_NAD-dep_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11728. PTHR11728. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PRINTS	PR00077. GPDHDRGNASE.
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPDH3_ORYSJ

Accession

Primary (citable) accession number: Q65X70
Secondary accession number(s): Q0DH26

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2012
Last sequence update:	October 25, 2004
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program