Q65WL7 (ASNA_MANSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	MS0036

Organism

Mannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]

Taxonomic identifier

221988 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Basfia

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017954

Sequences

	Sequence		Length	Mass (Da)
	Q65WL7 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: A6F365E612908801	FASTA	330	37,364
	10 20 30 40 50 60 MKKSFILQQQ EISFTKNTFT EKLAEHLGLV EVQGPILSQV GNGIQDNLSG TEKAVQVNVK 70 80 90 100 110 120 MITDAAFEVV HSLAKWKRHT LARFGFAEGE GLFVHMKALR PDEDSLDQTH SVYVDQWDWE 130 140 150 160 170 180 KVIPEGRRNL DYLKETVREI YAAILETEAA VDKKYGLKSF LPKEITFIHS EDLVKDYPGM 190 200 210 220 230 240 TDKERENELC KKYGAVFLIG IGGVLPDGKP HDGRAPDYDD WTTTSEGEYK GLNGDILVWN 250 260 270 280 290 300 PILNRAFEVS SMGIRVDETA LRKQLSITGD EDRLKFDWHQ DLINGRMPLS IGGGIGQSRL 310 320 330 AMLLLQKRHI GEVQSSVWPK AVMEQYENIL

References

[1]

"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens." Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J. Nat. Biotechnol. 22:1275-1281(2004) [PubMed: 15378067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MBEL55E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016827 Genomic DNA. Translation: AAU36643.1.
RefSeq	YP_087228.1. NC_006300.1.
3D structure databases
HSSP	HSSP built from PDB template 12AS based on UniProtKB P00963.
ProteinModelPortal	Q65WL7.
SMR	Q65WL7. Positions 4-330.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65WL7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3077137.
GenomeReviews	Gene locus MS0036 in contig AE016827_GR.
KEGG	msu:MS0036.
NMPDR	fig|221988.1.peg.36.
PATRIC	22443525. VBIManSuc86752_0034.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2502.
HOGENOM	HBG288146.
OMA	LNDNLNG.
PhylomeDB	Q65WL7.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	MSUC221988:MS0036-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_MANSM

Accession

Primary (citable) accession number: Q65WL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program