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Q65VT5

- TRMB_MANSM

UniProt

Q65VT5 - TRMB_MANSM

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801S-adenosyl-L-methionineUniRule annotation
Binding sitei105 – 1051S-adenosyl-L-methionineUniRule annotation
Binding sitei132 – 1321S-adenosyl-L-methionineUniRule annotation
Active sitei155 – 1551By similarity
Binding sitei155 – 1551S-adenosyl-L-methionineUniRule annotation
Binding sitei159 – 1591SubstrateUniRule annotation
Binding sitei191 – 1911SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBMAN221988:GHGM-324-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:MS0318
OrganismiMannheimia succiniciproducens (strain MBEL55E)
Taxonomic identifieri221988 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
ProteomesiUP000000607: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249tRNA (guanine-N(7)-)-methyltransferasePRO_0000171346Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi221988.MS0318.

Structurei

3D structure databases

ProteinModelPortaliQ65VT5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni228 – 2314Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65VT5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEQKITFAD QKRKTVETAE FTEDGRYKRK VRSFVLRTGR LSEFQRNMMN
60 70 80 90 100
DNWADFGLEH QNNYFDFAEI YGNTNPVILE IGFGMGKSLV EMAEQNPERN
110 120 130 140 150
YLGIEVHTPG VGACIAYAVE KQVKNLRVIC HDATEILQDC IADDSLGGLQ
160 170 180 190 200
LFFPDPWHKS KHHKRRIVQP NFVDNVMQKL QQSGFIHMAT DWENYAEQML
210 220 230 240
DVLSQSKALT NTSKTNDFIP RPDFRPLTKF EQRGHRLGHG VWDLYFVKN
Length:249
Mass (Da):28,908
Last modified:May 29, 2007 - v2
Checksum:i7B6D09F79B9F9E09
GO

Sequence cautioni

The sequence AAU36925.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016827 Genomic DNA. Translation: AAU36925.1. Different initiation.
RefSeqiYP_087510.1. NC_006300.1.

Genome annotation databases

EnsemblBacteriaiAAU36925; AAU36925; MS0318.
GeneIDi3076119.
KEGGimsu:MS0318.
PATRICi22444056. VBIManSuc86752_0293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016827 Genomic DNA. Translation: AAU36925.1 . Different initiation.
RefSeqi YP_087510.1. NC_006300.1.

3D structure databases

ProteinModelPortali Q65VT5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 221988.MS0318.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU36925 ; AAU36925 ; MS0318 .
GeneIDi 3076119.
KEGGi msu:MS0318.
PATRICi 22444056. VBIManSuc86752_0293.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073968.
KOi K03439.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci BMAN221988:GHGM-324-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
    Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
    Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MBEL55E.

Entry informationi

Entry nameiTRMB_MANSM
AccessioniPrimary (citable) accession number: Q65VT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: May 29, 2007
Last modified: October 1, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3