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Q65VT5

- TRMB_MANSM

UniProt

Q65VT5 - TRMB_MANSM

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 2 (29 May 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei80 – 801S-adenosyl-L-methionineUniRule annotation
    Binding sitei105 – 1051S-adenosyl-L-methionineUniRule annotation
    Binding sitei132 – 1321S-adenosyl-L-methionineUniRule annotation
    Active sitei155 – 1551By similarity
    Binding sitei155 – 1551S-adenosyl-L-methionineUniRule annotation
    Binding sitei159 – 1591SubstrateUniRule annotation
    Binding sitei191 – 1911SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBMAN221988:GHGM-324-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:MS0318
    OrganismiMannheimia succiniciproducens (strain MBEL55E)
    Taxonomic identifieri221988 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
    ProteomesiUP000000607: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 249249tRNA (guanine-N(7)-)-methyltransferasePRO_0000171346Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi221988.MS0318.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65VT5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni228 – 2314Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q65VT5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEQKITFAD QKRKTVETAE FTEDGRYKRK VRSFVLRTGR LSEFQRNMMN    50
    DNWADFGLEH QNNYFDFAEI YGNTNPVILE IGFGMGKSLV EMAEQNPERN 100
    YLGIEVHTPG VGACIAYAVE KQVKNLRVIC HDATEILQDC IADDSLGGLQ 150
    LFFPDPWHKS KHHKRRIVQP NFVDNVMQKL QQSGFIHMAT DWENYAEQML 200
    DVLSQSKALT NTSKTNDFIP RPDFRPLTKF EQRGHRLGHG VWDLYFVKN 249
    Length:249
    Mass (Da):28,908
    Last modified:May 29, 2007 - v2
    Checksum:i7B6D09F79B9F9E09
    GO

    Sequence cautioni

    The sequence AAU36925.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016827 Genomic DNA. Translation: AAU36925.1. Different initiation.
    RefSeqiYP_087510.1. NC_006300.1.

    Genome annotation databases

    EnsemblBacteriaiAAU36925; AAU36925; MS0318.
    GeneIDi3076119.
    KEGGimsu:MS0318.
    PATRICi22444056. VBIManSuc86752_0293.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016827 Genomic DNA. Translation: AAU36925.1 . Different initiation.
    RefSeqi YP_087510.1. NC_006300.1.

    3D structure databases

    ProteinModelPortali Q65VT5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 221988.MS0318.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU36925 ; AAU36925 ; MS0318 .
    GeneIDi 3076119.
    KEGGi msu:MS0318.
    PATRICi 22444056. VBIManSuc86752_0293.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci BMAN221988:GHGM-324-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
      Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
      Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MBEL55E.

    Entry informationi

    Entry nameiTRMB_MANSM
    AccessioniPrimary (citable) accession number: Q65VT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 15, 2005
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3