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Q65VT5 (TRMB_MANSM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:MS0318
OrganismMannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]
Taxonomic identifier221988 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia

Protein attributes

Sequence length249 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Sequence caution

The sequence AAU36925.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 249249tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171346

Regions

Region228 – 2314Substrate binding By similarity

Sites

Active site1551 By similarity
Binding site801S-adenosyl-L-methionine By similarity
Binding site1051S-adenosyl-L-methionine By similarity
Binding site1321S-adenosyl-L-methionine By similarity
Binding site1551S-adenosyl-L-methionine By similarity
Binding site1591Substrate By similarity
Binding site1911Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65VT5 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: 7B6D09F79B9F9E09

FASTA24928,908
        10         20         30         40         50         60 
MSEQKITFAD QKRKTVETAE FTEDGRYKRK VRSFVLRTGR LSEFQRNMMN DNWADFGLEH 

        70         80         90        100        110        120 
QNNYFDFAEI YGNTNPVILE IGFGMGKSLV EMAEQNPERN YLGIEVHTPG VGACIAYAVE 

       130        140        150        160        170        180 
KQVKNLRVIC HDATEILQDC IADDSLGGLQ LFFPDPWHKS KHHKRRIVQP NFVDNVMQKL 

       190        200        210        220        230        240 
QQSGFIHMAT DWENYAEQML DVLSQSKALT NTSKTNDFIP RPDFRPLTKF EQRGHRLGHG 


VWDLYFVKN 

« Hide

References

[1]"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MBEL55E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016827 Genomic DNA. Translation: AAU36925.1. Different initiation.
RefSeqYP_087510.1. NC_006300.1.

3D structure databases

ProteinModelPortalQ65VT5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING221988.MS0318.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU36925; AAU36925; MS0318.
GeneID3076119.
KEGGmsu:MS0318.
PATRIC22444056. VBIManSuc86752_0293.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycBMAN221988:GHGM-324-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MANSM
AccessionPrimary (citable) accession number: Q65VT5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: May 29, 2007
Last modified: April 16, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways