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Q65VR5 (SYL_MANSM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MS0338
OrganismMannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]
Taxonomic identifier221988 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia

Protein attributes

Sequence length860 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 860860Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152040

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65VR5 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: A5D49243B05C04C4

FASTA86097,814
        10         20         30         40         50         60 
MQEQYRPDLL EQEVQKYWQN NQTFKAVKDS SKEKYYCLSM FPYPSGRLHM GHVRNYTIAD 

        70         80         90        100        110        120 
VVSRYQRMNG KNVLQPVGWD AFGLPAEGAA VKNKTAPAKW TYENIDYMKN QLKMLGFSYD 

       130        140        150        160        170        180 
WDREIATCKP EYYKWEQWFF TELYKKGLVY KKTSVVNWCP NDETVLANEQ VHEGCCWRCD 

       190        200        210        220        230        240 
TPVEQKEIPQ WFIKITDYAE QLLSGLDTLP EWPDMVKTMQ RNWIGRSEGV EITFKIENSD 

       250        260        270        280        290        300 
ETVAVYTTRP DTFYGVSYMA VAAGHPLAEK AAQNNAELAR FIQECKNTKV AEAELATMEK 

       310        320        330        340        350        360 
KGMATGINAI HPITGKPVPV WVANFVLMHY GTGAVMAVPA HDQRDFEFAT KYGLPIKQVI 

       370        380        390        400        410        420 
APMNGEEIDL TKAAFTEHGK LVNSAEFDGL DFEAAFNGIA DKLEKMGVGK RQVNYRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMLTLENG DVVPAPLQDL PIVLPEDVVM DGVKSPIKAD PDWAKTSYNG 

       490        500        510        520        530        540 
QPALKETDTF DTFMESSWYY ARYTSPQYHE GMLDSDEANY WLPVDQYIGG IEHATMHLLY 

       550        560        570        580        590        600 
FRFFHKLLRD AGLVSTDEPT KKLLCQGMVL ADAFYYTSPT NERIWVSPTK VMLERDEKGR 

       610        620        630        640        650        660 
ILKATDDEGH ELVHAGMTKM SKSKNNGIDP QEMVEKYGAD TVRLFMMFAS PAEMTLEWQE 

       670        680        690        700        710        720 
SGVEGAKRFL GRLWNLVFEY NKNPVKTAPN PTALSSAQKA LRRDVHKTIA KVSDDIGRRQ 

       730        740        750        760        770        780 
TFNTAIAAIM ELMNKLTRAP LTDEQDRAVM GEALSAVVRM LYPITPHICF QLWKDLGNED 

       790        800        810        820        830        840 
IIDFAPWVQA DEAAMIDDEK LVVVQVNGKV RGKITVPADM AEEEIKRVAL AEENVQKFLD 

       850        860 
GLNIVKVIYV PGKLLSFVAK 

« Hide

References

[1]"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MBEL55E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016827 Genomic DNA. Translation: AAU36945.1.
RefSeqYP_087530.1. NC_006300.1.

3D structure databases

ProteinModelPortalQ65VR5.
SMRQ65VR5. Positions 228-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING221988.MS0338.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU36945; AAU36945; MS0338.
GeneID3076196.
KEGGmsu:MS0338.
PATRIC22444096. VBIManSuc86752_0313.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBMAN221988:GHGM-344-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MANSM
AccessionPrimary (citable) accession number: Q65VR5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 25, 2004
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries