Reviewed, UniProtKB/Swiss-Prot Q65VK7 (NAGZ_MANSM)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase
    Beta-N-acetylhexosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	MS0396

Organism

Mannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]

Taxonomic identifier

221988 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Mannheimia

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_00364
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Beta-hexosaminidase HAMAP MF_00364		PRO_0000234915
Sites
	Active site	249	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65VK7-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: C75099A6C2D8DAA2	FASTA	347	38,847
	10 20 30 40 50 60 MSTLLIDLKG QELLAEEAEL LAHPLVAGLI LFTRNFYDRS QIQALIKDIR RRVKKPLLIT 70 80 90 100 110 120 VDQEGGRVQR FREGFTQLPA MQSFAAMISD PALQLTTAKE AGWLMAAEMT ALDIDLSFAP 130 140 150 160 170 180 VLDLGHECKA IGDRSFCEEV EPAVRLASAF IDGMHQAGMA TTGKHFPGHG HVLADSHLET 190 200 210 220 230 240 PYDERPSAVI FERDIQPFQQ LIAQNKLDAV MPAHVIYRHC DSQPASGSKY WLQDILRQKL 250 260 270 280 290 300 GFDGTVFSDD LGMKGAGFMG DFVARSEKAL SAGCDLLLLC NEPEGVVQVL DNLKLEENPP 310 320 330 340 HFAARQRRLQ SLFKKKAFSW NELTKTRRWL ENSKKLTALQ QSWLDSK

References

[1]

"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens." Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J. Nat. Biotechnol. 22:1275-1281(2004) [PubMed: 15378067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016827 Genomic DNA. Translation: AAU37003.1.
RefSeq	YP_087588.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q65VK7.
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Genome annotation databases
GeneID	3075871.
GenomeReviews	Gene locus MS0396 in contig AE016827_GR.
KEGG	msu:MS0396.
NMPDR	fig|221988.1.peg.377.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65VK7.
OMA	WQYMANT.
Family and domain databases
HAMAP	MF_00364. [Tree]
InterPro	IPR019800. Glyco_hydro_3_AS. IPR001764. Glyco_hydro_3_N. [Graphical view]
Gene3D	G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGZ_MANSM

Accession

Primary (citable) accession number: Q65VK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)