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Q65VJ8 (TYSY_MANSM) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:MS0405
OrganismMannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]
Taxonomic identifier221988 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Basfia

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008

Subunit structure

Homodimer By similarity. HAMAP MF_00008

Subcellular location

Cytoplasm By similarity HAMAP MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdTMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionthymidylate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 283283Thymidylate synthase HAMAP MF_00008
PRO_0000140978

Sites

Active site1601 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65VJ8 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: ED695E7F6DDD865B

FASTA28332,687
        10         20         30         40         50         60 
MRQYLDLCQR IVNEGCWIEN KRTGKRCLTV INADLTYDVA NNRFPIITTR KSYWKAAIAE 

        70         80         90        100        110        120 
FLGYIRGYDN AADFRKLGAK TWDANANENQ VWLNNPHRKG TDDMGRVYGV QGRAWRKPNG 

       130        140        150        160        170        180 
ETVDQLRKIV NNLSRGIDDR GEILTFLNPG EFDLGCLRPC MYNHTFSLLG DTLYLTSYQR 

       190        200        210        220        230        240 
SCDVPLGLNF NQIQVFTFLA LMAQITGKKA GQAYHKIVNA HIYEDQLELM RDVQLKREPF 

       250        260        270        280 
PSPKLEINPD IKTLEDLETW VTMDDFNVVG YQCHEPIKYP FSV

« Hide

References

[1]"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
Nat. Biotechnol. 22:1275-1281(2004) [PubMed: 15378067] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MBEL55E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016827 Genomic DNA. Translation: AAU37012.1.
RefSeqYP_087597.1. NC_006300.1.

3D structure databases

ProteinModelPortalQ65VJ8.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ65VJ8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3075186.
GenomeReviewsGene locus MS0405 in contig AE016827_GR.
KEGGmsu:MS0405.
NMPDRfig|221988.1.peg.385.
PATRIC22444212. VBIManSuc86752_0371.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHBG588098.
OMAPIKYPFS.
PhylomeDBQ65VJ8.
ProtClustDBPRK01827.

Enzyme and pathway databases

BioCycMSUC221988:MS0405-MONOMER.

Family and domain databases

HAMAPMF_00008. Thymidy_synth_bact.
[Tree]
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
Gene3DG3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KOK00560.
PANTHERPTHR11549:SF2. Thymidylat_synth_C. 1 hit.
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMsTIGR03284. Thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_MANSM
AccessionPrimary (citable) accession number: Q65VJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 25, 2004
Last modified: January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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