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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi110ZincUniRule annotation1
Metal bindingi112ZincUniRule annotation1
Metal bindingi137ZincUniRule annotation1
Metal bindingi139ZincUniRule annotation1
Binding sitei251ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMSUC221988:G1G0A-461-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:MS0490
OrganismiMannheimia succiniciproducens (strain MBEL55E)
Taxonomic identifieri221988 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001195951 – 480Glutamate--tRNA ligaseAdd BLAST480

Proteomic databases

PRIDEiQ65VB3

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi221988.MS0490

Structurei

3D structure databases

ProteinModelPortaliQ65VB3
SMRiQ65VB3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi21 – 31"HIGH" regionUniRule annotationAdd BLAST11
Motifi248 – 252"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q65VB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELKALFNLD PNVKVRTRFA PSPTGYLHVG GARTALYSWL YAKHNDGEFV
60 70 80 90 100
LRIEDTDLER STPEATAAIL DAMEWLNLTW EHGPYFQTER FDRYNEVIDQ
110 120 130 140 150
MIEQGLAYRC YCSKERLEEL RHQQEANKEK PRYDRHCLHD HEHSPYEPHV
160 170 180 190 200
VRFKNPQEGS VVFEDAVRGR IEISNHELDD LIIRRSDGSP TYNFCVVVDD
210 220 230 240 250
WDMGITHVVR GEDHINNTPR QINILKALGA PIPVYAHVSM INGDDGQKLS
260 270 280 290 300
KRHGAVSVMQ YRDEGYLPEA LLNYLVRLGW GHGDQEIFTL EEMIKLFELE
310 320 330 340 350
HVSKSASAFN TEKLLWLNQH YIRELPAEYV AQHLAWQYQE QGIDTSKGPA
360 370 380 390 400
LTEIVSMLGE RCKTLKEMAA SSRYFFEEFD GFDEAAAKKH LKAAAVEPLE
410 420 430 440 450
KVKEKLTALS GWDAHSAHEA IEQTAAELEV GMGKVGMPLR VAVTGAGQSP
460 470 480
SMDVTLAGIG RERVLARIQK AIDFIKAKNA
Length:480
Mass (Da):54,588
Last modified:March 24, 2009 - v3
Checksum:i36E148D4CA10F528
GO

Sequence cautioni

The sequence AAU37097 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016827 Genomic DNA Translation: AAU37097.1 Different initiation.
RefSeqiWP_041639542.1, NC_006300.1

Genome annotation databases

EnsemblBacteriaiAAU37097; AAU37097; MS0490
KEGGimsu:MS0490

Entry informationi

Entry nameiSYE_MANSM
AccessioniPrimary (citable) accession number: Q65VB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 24, 2009
Last modified: May 23, 2018
This is version 87 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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