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Q65VB3

- SYE_MANSM

UniProt

Q65VB3 - SYE_MANSM

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Protein
Glutamate--tRNA ligase
Gene
gltX, MS0490
Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi110 – 1101Zinc By similarity
Metal bindingi112 – 1121Zinc By similarity
Metal bindingi137 – 1371Zinc By similarity
Metal bindingi139 – 1391Zinc By similarity
Binding sitei251 – 2511ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBMAN221988:GHGM-511-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gltX
Ordered Locus Names:MS0490
OrganismiMannheimia succiniciproducens (strain MBEL55E)
Taxonomic identifieri221988 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
ProteomesiUP000000607: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Glutamate--tRNA ligaseUniRule annotation
PRO_0000119595Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi221988.MS0490.

Structurei

3D structure databases

ProteinModelPortaliQ65VB3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi21 – 3111"HIGH" regionUniRule annotation
Add
BLAST
Motifi248 – 2525"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65VB3-1 [UniParc]FASTAAdd to Basket

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MELKALFNLD PNVKVRTRFA PSPTGYLHVG GARTALYSWL YAKHNDGEFV    50
LRIEDTDLER STPEATAAIL DAMEWLNLTW EHGPYFQTER FDRYNEVIDQ 100
MIEQGLAYRC YCSKERLEEL RHQQEANKEK PRYDRHCLHD HEHSPYEPHV 150
VRFKNPQEGS VVFEDAVRGR IEISNHELDD LIIRRSDGSP TYNFCVVVDD 200
WDMGITHVVR GEDHINNTPR QINILKALGA PIPVYAHVSM INGDDGQKLS 250
KRHGAVSVMQ YRDEGYLPEA LLNYLVRLGW GHGDQEIFTL EEMIKLFELE 300
HVSKSASAFN TEKLLWLNQH YIRELPAEYV AQHLAWQYQE QGIDTSKGPA 350
LTEIVSMLGE RCKTLKEMAA SSRYFFEEFD GFDEAAAKKH LKAAAVEPLE 400
KVKEKLTALS GWDAHSAHEA IEQTAAELEV GMGKVGMPLR VAVTGAGQSP 450
SMDVTLAGIG RERVLARIQK AIDFIKAKNA 480
Length:480
Mass (Da):54,588
Last modified:March 24, 2009 - v3
Checksum:i36E148D4CA10F528
GO

Sequence cautioni

The sequence AAU37097.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016827 Genomic DNA. Translation: AAU37097.1. Different initiation.
RefSeqiYP_087682.1. NC_006300.1.

Genome annotation databases

EnsemblBacteriaiAAU37097; AAU37097; MS0490.
GeneIDi3075217.
KEGGimsu:MS0490.
PATRICi22444404. VBIManSuc86752_0452.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016827 Genomic DNA. Translation: AAU37097.1 . Different initiation.
RefSeqi YP_087682.1. NC_006300.1.

3D structure databases

ProteinModelPortali Q65VB3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 221988.MS0490.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU37097 ; AAU37097 ; MS0490 .
GeneIDi 3075217.
KEGGi msu:MS0490.
PATRICi 22444404. VBIManSuc86752_0452.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252722.
KOi K01885.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci BMAN221988:GHGM-511-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
    Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
    Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MBEL55E.

Entry informationi

Entry nameiSYE_MANSM
AccessioniPrimary (citable) accession number: Q65VB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 67 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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