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Q65VB0

- PNP_MANSM

UniProt

Q65VB0 - PNP_MANSM

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, MS0493
Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi485 – 4851Magnesium By similarity
Metal bindingi491 – 4911Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciBMAN221988:GHGM-514-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:MS0493
OrganismiMannheimia succiniciproducens (strain MBEL55E)
Taxonomic identifieri221988 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
ProteomesiUP000000607: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 704704Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_0000329707Add
BLAST

Proteomic databases

PRIDEiQ65VB0.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Protein-protein interaction databases

STRINGi221988.MS0493.

Structurei

3D structure databases

ProteinModelPortaliQ65VB0.
SMRiQ65VB0. Positions 616-690.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini552 – 61160KHAdd
BLAST
Domaini621 – 68969S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65VB0-1 [UniParc]FASTAAdd to Basket

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MNPIVKQFKY GQHTVTLETG AIARQATAAV MASMDDTTVF VSVVAKKDVK    50
EGQDFFPLTV DYQERTYAAG KIPGGFFKRE GRPSEGETLI ARLIDRPIRP 100
LFPEGFLNEI QIIATVVSVN PQISPDLVAM IGASAALSLS GVPFNGPIGA 150
ARVGFINDQF VLNPTMAEQK QSRLDLVVAG TDKAVLMVES EADILTEEQM 200
LAAVVFGHQQ QQVVVEAIKE FVAEAGKPRW DWVAPEPDSA LISKVKAIAE 250
NRLGDAYRIT EKQVRYEQID AIKADVIAQI TAEDEEVSEG KIVDIFTALE 300
SQIVRGRIIA GEPRIDGRTV DTVRALDICT GVLPRTHGSA IFTRGETQSL 350
AVVTLGTERD AQILDELTGE RQDTFLFHYN FPPYSVGETG RVGSPKRREI 400
GHGRLAKRGV AAVMPSISEF PYVVRVVSEI TESNGSSSMA SVCGASLALM 450
DAGVPVKSAV AGIAMGLVKE DDKFVVLSDI LGDEDHLGDM DFKVAGTRTG 500
VTALQMDIKI EGITPEIMQI ALNQAKSARM HILGVMEQAI SAPRAEISEF 550
APRIYTMKID PKKIKDVIGK GGATIRALTE ETGTSIDIDD DGTVKIAAVD 600
GNAVKTVMAR IEDITAEVEA GAVYTGKVTR LADFGAFVAI VGNKEGLVHI 650
SQIAEERVEK VSDYLQVGQE VQVKVVEIDR QGRIRLTMRD LGSKEESQEL 700
SVEQ 704
Length:704
Mass (Da):76,152
Last modified:October 25, 2004 - v1
Checksum:i4C9A56D23B6340F7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016827 Genomic DNA. Translation: AAU37100.1.
RefSeqiWP_011199672.1. NC_006300.1.
YP_087685.1. NC_006300.1.

Genome annotation databases

EnsemblBacteriaiAAU37100; AAU37100; MS0493.
GeneIDi3076357.
KEGGimsu:MS0493.
PATRICi22444408. VBIManSuc86752_0453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016827 Genomic DNA. Translation: AAU37100.1 .
RefSeqi WP_011199672.1. NC_006300.1.
YP_087685.1. NC_006300.1.

3D structure databases

ProteinModelPortali Q65VB0.
SMRi Q65VB0. Positions 616-690.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 221988.MS0493.

Proteomic databases

PRIDEi Q65VB0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU37100 ; AAU37100 ; MS0493 .
GeneIDi 3076357.
KEGGi msu:MS0493.
PATRICi 22444408. VBIManSuc86752_0453.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci BMAN221988:GHGM-514-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
    Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
    Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MBEL55E.

Entry informationi

Entry nameiPNP_MANSM
AccessioniPrimary (citable) accession number: Q65VB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 25, 2004
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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