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Q65UP2 (RUVC_MANSM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:MS0711
OrganismMannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]
Taxonomic identifier221988 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia

Protein attributes

Sequence length199 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 199199Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000183108

Sites

Metal binding171Magnesium By similarity
Metal binding761Magnesium By similarity
Metal binding1481Magnesium By similarity
Metal binding1511Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65UP2 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 05B9B63F5A476C3B

FASTA19921,914
        10         20         30         40         50         60 
MFALFIWSFM AIILGIDPGS RVTGYGIIRQ TGRTLEYLGS GAIRTQVEDL PTRLKRIYAG 

        70         80         90        100        110        120 
VTEIITQFRP DMFAIEEVFL AKNPNSALKL GQARGTAIVA AVNQNLPVFE YAARLVKQTV 

       130        140        150        160        170        180 
TGSGSADKVQ VQDMVTRILR LSDKPQADAA DALAIAITHA HTIQHSLQVA TSAKSTENHE 

       190 
KTTALLRTRY SRGRFRLKI 

« Hide

References

[1]"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MBEL55E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016827 Genomic DNA. Translation: AAU37318.1.
RefSeqYP_087903.1. NC_006300.1.

3D structure databases

ProteinModelPortalQ65UP2.
SMRQ65UP2. Positions 11-168.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING221988.MS0711.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU37318; AAU37318; MS0711.
GeneID3075058.
KEGGmsu:MS0711.
PATRIC22444811. VBIManSuc86752_0652.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAILHMIKL.
OrthoDBEOG6RG044.
ProtClustDBPRK00039.

Enzyme and pathway databases

BioCycBMAN221988:GHGM-734-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_MANSM
AccessionPrimary (citable) accession number: Q65UP2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 25, 2004
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families