Reviewed, UniProtKB/Swiss-Prot Q65UJ9 (ARGA_MANSM)

Names and origin

Protein names

Recommended name:
    Amino-acid acetyltransferase
    EC=2.3.1.1
Alternative name(s):
    N-acetylglutamate synthase
      Short name=AGS
      Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	MS0754

Organism

Mannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]

Taxonomic identifier

221988 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Mannheimia

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm By similarity.
Miscellaneous	In bacteria which possess the bifunctional enzyme ornithine acetyltransferase/N-acetylglutamate synthase (argJ), argA fulfills an anaplerotic role. HAMAP MF_01105
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino-acid N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Amino-acid acetyltransferase HAMAP MF_01105		PRO_1000084813
Regions
	Domain	289 – 429	141	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	Q65UJ9-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 593FD647EBBD24A5	FASTA	439	49,472
	10 20 30 40 50 60 MRSTELVQWF RQSTPYVNMH RGKTFVIMLD GNTIASSNFI NIINDISLLH SLGIKLIIVY 70 80 90 100 110 120 GARVQINSLL AQNNVTSVYH KNIRVTDPRT LELVKQAVGQ LSYDITARLS VRLPHSPVLN 130 140 150 160 170 180 VVSSNFILAQ PIGVDDGVDY MLSGKIRRIE IDNIKHHLDN NAIVLLGPIA PSVTGETFNL 190 200 210 220 230 240 PFEEIATQVA IKLKAEKLIG FSSTQGILDP QGISIPDLLP QDAAKYLNQY IQQGEYHCSQ 250 260 270 280 290 300 ARFLQAAIEV CKAGVKRSHL LSYEEDGSLL QELFTRDGVG TQLSVDNSED IRIATVQDIP 310 320 330 340 350 360 GLIELIHPLE QQGILVKRSR EQLEMDIANY TIIDRDGVII ACAALNQYPE ENMAEMACVA 370 380 390 400 410 420 VHPDYRSSSR GDILLEAIQK RARQLGIEKL FVLTTRTVHW FQERGFRLAN VEDLPKEKRD 430 HYNYQRRSKI LIQPLNEEE

References

[1]

"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens." Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J. Nat. Biotechnol. 22:1275-1281(2004) [PubMed: 15378067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016827 Genomic DNA. Translation: AAU37361.1.
RefSeq	YP_087946.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q65UJ9.
Genome annotation databases
GeneID	3076769.
GenomeReviews	Gene locus MS0754 in contig AE016827_GR.
KEGG	msu:MS0754.
NMPDR	fig|221988.1.peg.713.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65UJ9.
OMA	DLIRPLE.
Family and domain databases
HAMAP	MF_01105. [Tree]
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR000182. GCN5-rel_AcTrfase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_MANSM

Accession

Primary (citable) accession number: Q65UJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)