ID   Q65TT6_MANSM            Unreviewed;       880 AA.
AC   Q65TT6;
DT   25-OCT-2004, integrated into UniProtKB/TrEMBL.
DT   25-OCT-2004, sequence version 1.
DT   27-MAR-2024, entry version 112.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595,
GN   ECO:0000313|EMBL:AAU37624.1};
GN   OrderedLocusNames=MS1017 {ECO:0000313|EMBL:AAU37624.1};
OS   Mannheimia succiniciproducens (strain MBEL55E).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Basfia.
OX   NCBI_TaxID=221988 {ECO:0000313|EMBL:AAU37624.1, ECO:0000313|Proteomes:UP000000607};
RN   [1] {ECO:0000313|EMBL:AAU37624.1, ECO:0000313|Proteomes:UP000000607}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MBEL55E {ECO:0000313|EMBL:AAU37624.1,
RC   ECO:0000313|Proteomes:UP000000607};
RX   PubMed=15378067; DOI=10.1038/nbt1010;
RA   Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H.,
RA   Jeong H., Hur C.G., Kim J.J.;
RT   "The genome sequence of the capnophilic rumen bacterium Mannheimia
RT   succiniciproducens.";
RL   Nat. Biotechnol. 22:1275-1281(2004).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC       ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR   EMBL; AE016827; AAU37624.1; -; Genomic_DNA.
DR   RefSeq; WP_011200194.1; NC_006300.1.
DR   AlphaFoldDB; Q65TT6; -.
DR   STRING; 221988.MS1017; -.
DR   KEGG; msu:MS1017; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_6; -.
DR   OrthoDB; 9768133at2; -.
DR   Proteomes; UP000000607; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW   Rule:MF_00595};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595}.
FT   ACT_SITE        138
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        545
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   880 AA;  99613 MW;  B67AA9DE11D8C59D CRC64;
     MTEEYLMMRN NINMLGRFLG ETIQEAQGDD ILELIENIRV LSRNSRSGDD KARAALLDTL
     STISADNIIP VARAFSQFLN LTNVAEQYQT MSRSHEDKVS AERSTAALFA RLKEQHVSQE
     EIIKTVQKLL IEIVLTAHPT EVTRRSLMHK QVEINKCLAQ LDHTDLTAEE QKNIEYKLLR
     LIAEAWHTNE IRTNRPTPLE EAKWGFAVIE NSLWEGLPAF IRKLNDAAVE HLNYALPVDL
     TPVRFSSWMG GDRDGNPFVT AKITREALQL ARWKAADLFL TDIQELCDEL SMTQCTAEFR
     EKYGDHLEPY RVVVKDLRSK LKNTLDYYND ILAGRIPPFK QDEIISEDQQ LWQPLYDCYQ
     SLTACGMRII ANGLLLDTLR RVRCFGVTLL RLDIRQESTR HSDAIGEITR YIGLGDYSQW
     TEDDKQAFLI RELSSRRPLI PHNWTPSEHT REILDTCKVI AKQPEGVISC YIISMARTAS
     DVLAVHLLLK EAGISYHLPV VPLFETLDDL DASKEVMTQL FNVGWYRGVI KNRQMIMIGY
     SDSAKDAGMM AASWAQYRAQ DALVKLCEQT GIELTLFHGR GGTVGRGGAP AHAALLSQPP
     RSLKNGLRVT EQGEMIRFKL GLPAIAAESL DLYASAILEA NLLPPPEPKA SWCRVMDELA
     VASCEIYRNV VRGDKDFVPY FRSATPEQEL AKLPLGSRPA KRNPNGGVES LRAIPWIFAW
     MQNRLMLPAW LGAGASIRQA MESGKAAVIE EMCNHWPFFN TRIGMLEMVF SKTDSWLSEY
     YDQRLVKKEL WYLGESLRKQ LSEDIATVLR LSGKGDQLMS DLPWVAESIA LRNVYTDPLN
     LLQVELLRRL RADPEHPNPD IEQALMITIT GIAAGMRNTG
//