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Q65TI0 (GLGB_MANSM) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:MS1123
OrganismMannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]
Taxonomic identifier221988 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7297291,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188714

Sites

Active site4051Nucleophile By similarity
Active site4581Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65TI0 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 723DDFB52E751887

FASTA72983,760
        10         20         30         40         50         60 
MKKLVAQSVI DAFFDGTHSD PFAVLGMHET HNGIEIRVLL PEAHRVIVID KETHKAVVEL 

        70         80         90        100        110        120 
ELVDERGFFN AIVPKANQFF AYELQVYWGK ESQILEDPYR FHPMINELDN WLLAEGSHLR 

       130        140        150        160        170        180 
PYEVLGAHFV EYDNVAGVNF RVWAPNAKRV SVVGDFNYWD GRRHPMRFHP ASGIWELFLP 

       190        200        210        220        230        240 
KVALGQLYKF ELIDSNNQLR LKADPYAFAA QLRPDTASQV SALPEIVEMT EKRRAANQSD 

       250        260        270        280        290        300 
KPISIYEVHL GSWRRNLENN FWLDYDEIAD ELIPYVKEMG FTHIELLPIS EYPFDGSWGY 

       310        320        330        340        350        360 
QPLGLYAPTS RFGTPDGFKR LIEKAHESGI NVILDWVPGH FPSDTHGLAA FDGTSLYEYA 

       370        380        390        400        410        420 
DPKEGYHQDW NTLIYNYGRH EVKNYLSGNA LYWVERFGLD GLRVDAVASM IYRDYSRRDG 

       430        440        450        460        470        480 
EWVPNQYGGR ENLEAIEFLK HTNYVLGTEL PGVAAIAEES TSFPGVTLPP EHGGLGFHYK 

       490        500        510        520        530        540 
WNMGWMNDTL EYMKLDPVYR QYHHGKMTFA MLYQYSENFV LPLSHDEVVH GKGSLITKMS 

       550        560        570        580        590        600 
GDTWQKFANL RAYYGYMWAF PGKKLLFMGN EFAQGREWNY QESLDWFLLD DGQGGGWHSG 

       610        620        630        640        650        660 
VQRLVKDLNK TYQNQTALFE LDTNPQGFEW LVVDDNQNSV FAFERRSKSG EVIIVVSNFT 

       670        680        690        700        710        720 
PVPRDNYRIG VNEPGKYEEI LNTDSAYYKG SNLGNYGEVI AEEIENHGKA QSISVMVPPL 


ATVYLRLKK 

« Hide

References

[1]"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MBEL55E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016827 Genomic DNA. Translation: AAU37730.1.
RefSeqYP_088315.1. NC_006300.1.

3D structure databases

ProteinModelPortalQ65TI0.
SMRQ65TI0. Positions 117-725.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING221988.MS1123.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU37730; AAU37730; MS1123.
GeneID3075873.
KEGGmsu:MS1123.
PATRIC22445593. VBIManSuc86752_1028.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycBMAN221988:GHGM-1159-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_MANSM
AccessionPrimary (citable) accession number: Q65TI0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: October 25, 2004
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries