Q65TI0 (GLGB_MANSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	MS1123

Organism

Mannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]

Taxonomic identifier

221988 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Basfia › Mannheimia succiniciproducens

Protein attributes

Sequence length	729 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-HAMAP
Molecular_function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: UniProtKB-HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 729	729	1,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685		PRO_0000188714
Sites
	Active site	405	1	Nucleophile By similarity
	Active site	458	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65TI0 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 723DDFB52E751887	FASTA	729	83,760
	10 20 30 40 50 60 MKKLVAQSVI DAFFDGTHSD PFAVLGMHET HNGIEIRVLL PEAHRVIVID KETHKAVVEL 70 80 90 100 110 120 ELVDERGFFN AIVPKANQFF AYELQVYWGK ESQILEDPYR FHPMINELDN WLLAEGSHLR 130 140 150 160 170 180 PYEVLGAHFV EYDNVAGVNF RVWAPNAKRV SVVGDFNYWD GRRHPMRFHP ASGIWELFLP 190 200 210 220 230 240 KVALGQLYKF ELIDSNNQLR LKADPYAFAA QLRPDTASQV SALPEIVEMT EKRRAANQSD 250 260 270 280 290 300 KPISIYEVHL GSWRRNLENN FWLDYDEIAD ELIPYVKEMG FTHIELLPIS EYPFDGSWGY 310 320 330 340 350 360 QPLGLYAPTS RFGTPDGFKR LIEKAHESGI NVILDWVPGH FPSDTHGLAA FDGTSLYEYA 370 380 390 400 410 420 DPKEGYHQDW NTLIYNYGRH EVKNYLSGNA LYWVERFGLD GLRVDAVASM IYRDYSRRDG 430 440 450 460 470 480 EWVPNQYGGR ENLEAIEFLK HTNYVLGTEL PGVAAIAEES TSFPGVTLPP EHGGLGFHYK 490 500 510 520 530 540 WNMGWMNDTL EYMKLDPVYR QYHHGKMTFA MLYQYSENFV LPLSHDEVVH GKGSLITKMS 550 560 570 580 590 600 GDTWQKFANL RAYYGYMWAF PGKKLLFMGN EFAQGREWNY QESLDWFLLD DGQGGGWHSG 610 620 630 640 650 660 VQRLVKDLNK TYQNQTALFE LDTNPQGFEW LVVDDNQNSV FAFERRSKSG EVIIVVSNFT 670 680 690 700 710 720 PVPRDNYRIG VNEPGKYEEI LNTDSAYYKG SNLGNYGEVI AEEIENHGKA QSISVMVPPL ATVYLRLKK

References

[1]

"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens." Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J. Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MBEL55E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016827 Genomic DNA. Translation: AAU37730.1.
RefSeq	YP_088315.1. NC_006300.1.
3D structure databases
ProteinModelPortal	Q65TI0.
SMR	Q65TI0. Positions 117-725.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	221988.MS1123.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU37730; AAU37730; MS1123.
GeneID	3075873.
KEGG	msu:MS1123.
PATRIC	22445593. VBIManSuc86752_1028.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HOG000283037.
KO	K00700.
OMA	AKLLFMG.
OrthoDB	EOG6JX7GT.
Enzyme and pathway databases
BioCyc	BMAN221988:GHGM-1159-MONOMER.
UniPathway	UPA00164.
Family and domain databases
Gene3D	2.60.40.10. 2 hits. 2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
HAMAP	MF_00685. GlgB.
InterPro	IPR006048. A-amylase_b_C. IPR006407. GlgB. IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. SSF51445. 1 hit. SSF81296. SSF81296. 2 hits.
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_MANSM

Accession

Primary (citable) accession number: Q65TI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	October 25, 2004
Last modified:	June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program