glgB

Functionⁱ

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityⁱ

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayⁱ: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	405	NucleophileUniRule annotation		1
Active siteⁱ	458	Proton donorUniRule annotation		1

GO - Molecular functionⁱ

1,4-alpha-glucan branching enzyme activity Source: UniProtKB-EC
1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis) Source: UniProtKB-EC
cation binding Source: InterPro
hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

glycogen biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase
Biological process	Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCycⁱ	MSUC221988:G1G0A-1048-MONOMER.
UniPathwayⁱ	UPA00164.

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.

CAZyⁱ

CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation) Alternative name(s): 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation Alpha-(1->4)-glucan branching enzymeUniRule annotation Glycogen branching enzymeUniRule annotation Short name: BEUniRule annotation
Gene namesⁱ	Name:glgBUniRule annotation Ordered Locus Names:MS1123
Organismⁱ	Mannheimia succiniciproducens (strain MBEL55E)
Taxonomic identifierⁱ	221988 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Basfia › [Mannheimia] succiniciproducens
Proteomesⁱ	UP000000607 Componentⁱ: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000188714	1 – 729	1,4-alpha-glucan branching enzyme GlgBAdd BLAST		729

Interactionⁱ

Subunit structureⁱ

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGⁱ	221988.MS1123.

STRINGⁱ

221988.MS1123.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q65TI0.
SMRⁱ	Q65TI0.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105C9C. Bacteria. COG0296. LUCA.
HOGENOMⁱ	HOG000283037.
KEGG Orthology (KO) More...KOⁱ	K00700.
OMAⁱ	EVVHGKS.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H08D1.

Family and domain databases

Gene3Dⁱ	2.60.40.10. 1 hit. 2.60.40.1180. 1 hit.
HAMAPⁱ	MF_00685. GlgB. 1 hit.
InterProⁱ	View protein in InterPro IPR006048. A-amylase/branching_C. IPR037439. Branching_enzy. IPR006407. GlgB. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013780. Glyco_hydro_b. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set.
PANTHERⁱ	PTHR43651. PTHR43651. 2 hits.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit.
PIRSFⁱ	PIRSF000463. GlgB. 1 hit.
SMARTⁱ	View protein in SMART SM00642. Aamy. 1 hit.
SUPFAMⁱ	SSF51445. SSF51445. 1 hit. SSF81296. SSF81296. 2 hits.
TIGRFAMsⁱ	TIGR01515. branching_enzym. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q65TI0-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKKLVAQSVI DAFFDGTHSD PFAVLGMHET HNGIEIRVLL PEAHRVIVID 
        60         70         80         90        100
KETHKAVVEL ELVDERGFFN AIVPKANQFF AYELQVYWGK ESQILEDPYR 
       110        120        130        140        150
FHPMINELDN WLLAEGSHLR PYEVLGAHFV EYDNVAGVNF RVWAPNAKRV 
       160        170        180        190        200
SVVGDFNYWD GRRHPMRFHP ASGIWELFLP KVALGQLYKF ELIDSNNQLR 
       210        220        230        240        250
LKADPYAFAA QLRPDTASQV SALPEIVEMT EKRRAANQSD KPISIYEVHL 
       260        270        280        290        300
GSWRRNLENN FWLDYDEIAD ELIPYVKEMG FTHIELLPIS EYPFDGSWGY 
       310        320        330        340        350
QPLGLYAPTS RFGTPDGFKR LIEKAHESGI NVILDWVPGH FPSDTHGLAA 
       360        370        380        390        400
FDGTSLYEYA DPKEGYHQDW NTLIYNYGRH EVKNYLSGNA LYWVERFGLD 
       410        420        430        440        450
GLRVDAVASM IYRDYSRRDG EWVPNQYGGR ENLEAIEFLK HTNYVLGTEL 
       460        470        480        490        500
PGVAAIAEES TSFPGVTLPP EHGGLGFHYK WNMGWMNDTL EYMKLDPVYR 
       510        520        530        540        550
QYHHGKMTFA MLYQYSENFV LPLSHDEVVH GKGSLITKMS GDTWQKFANL 
       560        570        580        590        600
RAYYGYMWAF PGKKLLFMGN EFAQGREWNY QESLDWFLLD DGQGGGWHSG 
       610        620        630        640        650
VQRLVKDLNK TYQNQTALFE LDTNPQGFEW LVVDDNQNSV FAFERRSKSG 
       660        670        680        690        700
EVIIVVSNFT PVPRDNYRIG VNEPGKYEEI LNTDSAYYKG SNLGNYGEVI 
       710        720 
AEEIENHGKA QSISVMVPPL ATVYLRLKK

Length:729

Mass (Da):83,760

Last modified:October 25, 2004 - v1

Checksum:ⁱ723DDFB52E751887

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE016827 Genomic DNA. Translation: AAU37730.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011200298.1. NC_006300.1.

Genome annotation databases

EnsemblBacteriaⁱ	AAU37730; AAU37730; MS1123.
KEGGⁱ	msu:MS1123.

Similar proteinsⁱ

50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q65TI0	Q3J3M6 A4WTG2 A0A239D4G3 A3PIQ5 UPI0002A3990E UPI000316DFA2 UPI00020DF9C9 A0A1E3CV59 UPI000C6E1874 UPI000C6DF6DE +689	Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) Rhodobacter megalophilus Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9) Rhodobacter sp. AKP1 Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) Rhodobacter johrii Rhodobacter azotoformans Rhodobacter sphaeroides (strain KD131 / KCTC 12085) Loktanella vestfoldensis And more	728	UniRef50_Q3J3M6	Cluster: 1,4-alpha-glucan branching enzyme GlgB	700

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE016827 Genomic DNA. Translation: AAU37730.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011200298.1. NC_006300.1.

3D structure databases

ProteinModelPortalⁱ	Q65TI0.
SMRⁱ	Q65TI0.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	221988.MS1123.

STRINGⁱ

221988.MS1123.

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.

CAZyⁱ

CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAU37730; AAU37730; MS1123.
KEGGⁱ	msu:MS1123.

Phylogenomic databases

eggNOGⁱ	ENOG4105C9C. Bacteria. COG0296. LUCA.
HOGENOMⁱ	HOG000283037.
KEGG Orthology (KO) More...KOⁱ	K00700.
OMAⁱ	EVVHGKS.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H08D1.

Enzyme and pathway databases

UniPathwayⁱ	UPA00164.
BioCycⁱ	MSUC221988:G1G0A-1048-MONOMER.

Family and domain databases

Gene3Dⁱ	2.60.40.10. 1 hit. 2.60.40.1180. 1 hit.
HAMAPⁱ	MF_00685. GlgB. 1 hit.
InterProⁱ	View protein in InterPro IPR006048. A-amylase/branching_C. IPR037439. Branching_enzy. IPR006407. GlgB. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013780. Glyco_hydro_b. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set.
PANTHERⁱ	PTHR43651. PTHR43651. 2 hits.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit.
PIRSFⁱ	PIRSF000463. GlgB. 1 hit.
SMARTⁱ	View protein in SMART SM00642. Aamy. 1 hit.
SUPFAMⁱ	SSF51445. SSF51445. 1 hit. SSF81296. SSF81296. 2 hits.
TIGRFAMsⁱ	TIGR01515. branching_enzym. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GLGB_MANSM
Accessionⁱ	Q65TI0Primary (citable) accession number: Q65TI0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
	Last sequence update:	October 25, 2004
	Last modified:	February 28, 2018
	This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q65TI0 (GLGB_MANSM)

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1,4-alpha-glucan branching enzyme GlgB

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycogen biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: glycogen biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ