UniProtKB - Q65TI0 (GLGB_MANSM)
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Protein
1,4-alpha-glucan branching enzyme GlgB
Gene
glgB
Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Functioni
Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation
Catalytic activityi
Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation
: glycogen biosynthesis Pathwayi
This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 405 | NucleophileUniRule annotation | 1 | |
Active sitei | 458 | Proton donorUniRule annotation | 1 |
GO - Molecular functioni
- 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-EC
- 1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis) Source: UniProtKB-EC
- cation binding Source: InterPro
- hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
GO - Biological processi
- glycogen biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism |
Enzyme and pathway databases
BioCyci | MSUC221988:G1G0A-1048-MONOMER. |
UniPathwayi | UPA00164. |
Protein family/group databases
CAZyi | CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13. |
Names & Taxonomyi
Protein namesi | Recommended name: 1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)Alternative name(s): 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation Alpha-(1->4)-glucan branching enzymeUniRule annotation Glycogen branching enzymeUniRule annotation Short name: BEUniRule annotation |
Gene namesi | Name:glgBUniRule annotation Ordered Locus Names:MS1123 |
Organismi | Mannheimia succiniciproducens (strain MBEL55E) |
Taxonomic identifieri | 221988 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Basfia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000188714 | 1 – 729 | 1,4-alpha-glucan branching enzyme GlgBAdd BLAST | 729 |
Interactioni
Subunit structurei
Monomer.UniRule annotation
Protein-protein interaction databases
STRINGi | 221988.MS1123. |
Structurei
3D structure databases
ProteinModelPortali | Q65TI0. |
SMRi | Q65TI0. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105C9C. Bacteria. COG0296. LUCA. |
HOGENOMi | HOG000283037. |
KOi | K00700. |
OMAi | EVVHGKS. |
OrthoDBi | POG091H08D1. |
Family and domain databases
Gene3Di | 2.60.40.10. 1 hit. 2.60.40.1180. 1 hit. |
HAMAPi | MF_00685. GlgB. 1 hit. |
InterProi | View protein in InterPro IPR006048. A-amylase/branching_C. IPR037439. Branching_enzy. IPR006407. GlgB. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013780. Glyco_hydro_b. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. |
PANTHERi | PTHR43651. PTHR43651. 2 hits. |
Pfami | View protein in Pfam PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. |
PIRSFi | PIRSF000463. GlgB. 1 hit. |
SMARTi | View protein in SMART SM00642. Aamy. 1 hit. |
SUPFAMi | SSF51445. SSF51445. 1 hit. SSF81296. SSF81296. 2 hits. |
TIGRFAMsi | TIGR01515. branching_enzym. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q65TI0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKLVAQSVI DAFFDGTHSD PFAVLGMHET HNGIEIRVLL PEAHRVIVID
60 70 80 90 100
KETHKAVVEL ELVDERGFFN AIVPKANQFF AYELQVYWGK ESQILEDPYR
110 120 130 140 150
FHPMINELDN WLLAEGSHLR PYEVLGAHFV EYDNVAGVNF RVWAPNAKRV
160 170 180 190 200
SVVGDFNYWD GRRHPMRFHP ASGIWELFLP KVALGQLYKF ELIDSNNQLR
210 220 230 240 250
LKADPYAFAA QLRPDTASQV SALPEIVEMT EKRRAANQSD KPISIYEVHL
260 270 280 290 300
GSWRRNLENN FWLDYDEIAD ELIPYVKEMG FTHIELLPIS EYPFDGSWGY
310 320 330 340 350
QPLGLYAPTS RFGTPDGFKR LIEKAHESGI NVILDWVPGH FPSDTHGLAA
360 370 380 390 400
FDGTSLYEYA DPKEGYHQDW NTLIYNYGRH EVKNYLSGNA LYWVERFGLD
410 420 430 440 450
GLRVDAVASM IYRDYSRRDG EWVPNQYGGR ENLEAIEFLK HTNYVLGTEL
460 470 480 490 500
PGVAAIAEES TSFPGVTLPP EHGGLGFHYK WNMGWMNDTL EYMKLDPVYR
510 520 530 540 550
QYHHGKMTFA MLYQYSENFV LPLSHDEVVH GKGSLITKMS GDTWQKFANL
560 570 580 590 600
RAYYGYMWAF PGKKLLFMGN EFAQGREWNY QESLDWFLLD DGQGGGWHSG
610 620 630 640 650
VQRLVKDLNK TYQNQTALFE LDTNPQGFEW LVVDDNQNSV FAFERRSKSG
660 670 680 690 700
EVIIVVSNFT PVPRDNYRIG VNEPGKYEEI LNTDSAYYKG SNLGNYGEVI
710 720
AEEIENHGKA QSISVMVPPL ATVYLRLKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE016827 Genomic DNA. Translation: AAU37730.1. |
RefSeqi | WP_011200298.1. NC_006300.1. |
Genome annotation databases
EnsemblBacteriai | AAU37730; AAU37730; MS1123. |
KEGGi | msu:MS1123. |
Similar proteinsi
Entry informationi
Entry namei | GLGB_MANSM | |
Accessioni | Q65TI0Primary (citable) accession number: Q65TI0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | October 25, 2004 | |
Last modified: | February 28, 2018 | |
This is version 96 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |