Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q65TC8 (TRUA_MANSM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase A

EC=5.4.99.12
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I
Gene names
Name:truA
Ordered Locus Names:MS1175
OrganismMannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]
Taxonomic identifier221988 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171

Catalytic activity

tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00171

Sequence similarities

Belongs to the tRNA pseudouridine synthase TruA family.

Sequence caution

The sequence AAU37782.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263tRNA pseudouridine synthase A HAMAP-Rule MF_00171
PRO_0000057405

Sites

Active site511Nucleophile By similarity
Binding site1091Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65TC8 [UniParc].

Last modified April 26, 2005. Version 2.
Checksum: C419C1B3B2F1D749

FASTA26329,955
        10         20         30         40         50         60 
MKIALGIEYN GKNYFGWQRQ EKVHSVQAEL EKALSFVANE KIEVFCAGRT DSGVHGTGQV 

        70         80         90        100        110        120 
VHFETNAIRP EKAWAFGTNA NLPDDIAVRW AKEVPDDFHA RFSATARRYR YVLYCNKLRS 

       130        140        150        160        170        180 
AILPYGITHT HLDLDEHKMQ EAGRFLLGEN DFSSFRAAQC QSNTPWRNIH HLNVIRRGNF 

       190        200        210        220        230        240 
VIVDIKANAF VHHMVRNIVG SLMEVGCGNQ PPEWIEWLLA QKNRKLAAPT AKAEGLYLVQ 

       250        260 
VTYPEHFELP QMPLGPLFLA DEL 

« Hide

References

[1]"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MBEL55E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016827 Genomic DNA. Translation: AAU37782.1. Different initiation.
RefSeqYP_088367.1. NC_006300.1.

3D structure databases

ProteinModelPortalQ65TC8.
SMRQ65TC8. Positions 2-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING221988.MS1175.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU37782; AAU37782; MS1175.
GeneID3076252.
KEGGmsu:MS1175.
PATRIC22445683. VBIManSuc86752_1073.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0101.
HOGENOMHOG000248672.
KOK06173.
OrthoDBEOG6Z9B4R.
ProtClustDBPRK00021.

Enzyme and pathway databases

BioCycBMAN221988:GHGM-1210-MONOMER.

Family and domain databases

Gene3D3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPMF_00171. TruA.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERPTHR11142. PTHR11142. 1 hit.
PfamPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMSSF55120. SSF55120. 1 hit.
TIGRFAMsTIGR00071. hisT_truA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRUA_MANSM
AccessionPrimary (citable) accession number: Q65TC8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: February 19, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families