Reviewed, UniProtKB/Swiss-Prot Q65T54 (CYSI_MANSM)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	MS1249

Organism

Mannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]

Taxonomic identifier

221988 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Mannheimia

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Sulfite reductase [NADPH] hemoprotein beta-component		PRO_0000199900
Sites
	Metal binding	443	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	449	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	488	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	492	1	Iron (siroheme axial ligand) By similarity
	Metal binding	492	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65T54-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: C687B9D48C5C0399	FASTA	588	67,221
	10 20 30 40 50 60 MSDKKQKGLE WQDNPLSDNE RLKEESNHLR GTILDDLEDG LTGGFKGDNF QLIRFHGMYE 70 80 90 100 110 120 QDDRDIRAER QEEKLEPRKF MLLRCRLPGG IIKPEQWIEI DKFARDNNYY QSIRLTNRQT 130 140 150 160 170 180 FQYHGVPKTK LQDMHRLLHK LGLDSIATAS DMNRNVLCSS NPVESELHQE AYEWAKKISE 190 200 210 220 230 240 HLLPRTNGYL DVWISGKKVQ SSDSFLGQED EPILGNRYLP RKYKTAVVLP PLNDVDLYSN 250 260 270 280 290 300 DMNFVGIKDE KTGKLAGFNV LVGGGLSFEH GNTKTYPNIA LELGYVPVED TLKAAESIVT 310 320 330 340 350 360 TQRDFGNRAD RKNARLRYTI QNMTLEGFRE EVERRMGRRF EAIRPFEFTE RGDRIGWVKG 370 380 390 400 410 420 IDKKWHLTCF IESGRLVDKP DLPLMTGMLE LAKVHKGDFR ITANQNIIIA NVAEEDKRQI 430 440 450 460 470 480 EDIARQYGLI RKITKLRENA MSCVSFPTCP LAMAESERVL PEFIDELDKI MAKHHVEQDY 490 500 510 520 530 540 IVTRITGCPN GCGRAMLAEI GLVGKAVGRY NLHLGGNIAG TRIPRLYKEN ITLDEILSEL 550 560 570 580 DGLIARWATE RDQGEGFGDF VLRVGIIKPV VNPVVDFWDE NLIPTVAV

References

[1]

"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens." Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J. Nat. Biotechnol. 22:1275-1281(2004) [PubMed: 15378067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016827 Genomic DNA. Translation: AAU37856.1.
RefSeq	YP_088441.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3075538.
GenomeReviews	Gene locus MS1249 in contig AE016827_GR.
KEGG	msu:MS1249.
NMPDR	fig|221988.1.peg.1170.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65T54.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_MANSM

Accession

Primary (citable) accession number: Q65T54

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	October 25, 2004
Last modified:	November 25, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)