Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciBMAN221988:GHGM-1346-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:MS1305
OrganismiMannheimia succiniciproducens (strain MBEL55E)
Taxonomic identifieri221988 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
ProteomesiUP000000607: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 875875Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192743Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi221988.MS1305.

Structurei

3D structure databases

ProteinModelPortaliQ65SZ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini461 – 594134HDUniRule annotationAdd
BLAST
Domaini700 – 78283ACT 1UniRule annotationAdd
BLAST
Domaini806 – 87570ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 343343UridylyltransferaseAdd
BLAST
Regioni344 – 699356Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiSLWNNWK.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65SZ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIGHNNFIIQ SVILRFFMFQ SVEGLLTPGL IKQQKEQLKQ TELENFAQAD
60 70 80 90 100
VNSLISHRTL FCDNFLIRLW RQFSLHEVTD LALIAVGGYG RREIFPLSDL
110 120 130 140 150
DFLILTEQPM PADLAKKVEE FIQFVWDCGF DVGASVRTLE DCDSQGRADI
160 170 180 190 200
TIATNLLESR LLTGNETLFD KLSSIVGRED FWPRKTFFEA KIQEKKQRYQ
210 220 230 240 250
RYNNTSYNLE PDIKYNPGGL RDLHLIYWIA LRHSNALSLE EILQSGFIYP
260 270 280 290 300
EEYAELERNQ QFLFKVRFAL HLILKRYDNR LLFDRQVKVS ELLGYQGEGN
310 320 330 340 350
QGVETMMKAF FQSLQAISLA SDILAKHYKE HFVDENGEEE CQVLDDNFQM
360 370 380 390 400
INNAIFLVRE DCFVQQPDTI LDLFSYLIIR PQAELHSSTL RLLHLALGQL
410 420 430 440 450
NGYLSELPAA REKFLRLLTQ PRGIERALIP MHKYGVLTAY IPEWKGIEGL
460 470 480 490 500
MQFDLFHIYT VDEHTMRVLA KLETFLSEET AEAHPLCVKL FPSLPDRALI
510 520 530 540 550
YIAALFHDIA KGRGGNHADL GAVDVGRFAA QHGFDCREIE TMKWLVKQHL
560 570 580 590 600
FMSVTAQRRD IHDPEVVMNF AAEVQNQVRL NYLVCLTVAD ICATNTTLWN
610 620 630 640 650
SWKRSLFASL YQYTNQQFNQ GMDNLLDNQE QEEQNKALAL EILQSQGFTE
660 670 680 690 700
DVQSLWKRCP GDYFLRNTPK ELAWHAVLLA GVETELLVKI SNRFSAGGTE
710 720 730 740 750
VFIYCKDRPN LFLKVVAAIG NKKLSIHDAQ IITSLDGYAF DSFIVTELDG
760 770 780 790 800
SLLKFDRRRV LEKAIINSLN SNELTKLQGS ENHKLQHFNV KTEVRFLNTE
810 820 830 840 850
KTTHTEMELF TLDKAGLLAD VSLVFSELNL SIQNAKITTI GEKAQDFFIL
860 870
TNAKGEALSE RERQSLSEKL QARLD
Length:875
Mass (Da):100,522
Last modified:October 25, 2004 - v1
Checksum:iE69E48587C5D349A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016827 Genomic DNA. Translation: AAU37912.1.
RefSeqiYP_088497.1. NC_006300.1.

Genome annotation databases

EnsemblBacteriaiAAU37912; AAU37912; MS1305.
GeneIDi3077152.
KEGGimsu:MS1305.
PATRICi22445929. VBIManSuc86752_1190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016827 Genomic DNA. Translation: AAU37912.1.
RefSeqiYP_088497.1. NC_006300.1.

3D structure databases

ProteinModelPortaliQ65SZ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi221988.MS1305.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU37912; AAU37912; MS1305.
GeneIDi3077152.
KEGGimsu:MS1305.
PATRICi22445929. VBIManSuc86752_1190.

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiSLWNNWK.
OrthoDBiEOG6CCH44.

Enzyme and pathway databases

BioCyciBMAN221988:GHGM-1346-MONOMER.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
    Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
    Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MBEL55E.

Entry informationi

Entry nameiGLND_MANSM
AccessioniPrimary (citable) accession number: Q65SZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: October 25, 2004
Last modified: January 7, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.