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Q65SH4 (ASSY_MANSM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:MS1479
OrganismMannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]
Taxonomic identifier221988 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00581

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00581

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00581

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00581.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 2 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444Argininosuccinate synthase HAMAP-Rule MF_00581
PRO_1000025431

Regions

Nucleotide binding18 – 269ATP By similarity

Sites

Binding site441ATP By similarity
Binding site1001Citrulline By similarity
Binding site1301ATP; via amide nitrogen By similarity
Binding site1321Aspartate By similarity
Binding site1321ATP By similarity
Binding site1361Aspartate By similarity
Binding site1361Citrulline By similarity
Binding site1371Aspartate By similarity
Binding site1371ATP By similarity
Binding site1401Citrulline By similarity
Binding site1931Citrulline By similarity
Binding site1951ATP By similarity
Binding site2021Citrulline By similarity
Binding site2041Citrulline By similarity
Binding site2811Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65SH4 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: F40DF4E2D0BA31BB

FASTA44449,466
        10         20         30         40         50         60 
MSNTILQNLP LGQKVGIAFS GGLDTSAALL WMRQKGAVPY AYTANLGQPD EDDYNAIPKK 

        70         80         90        100        110        120 
AMAYGAENAR LIDCRKQLAQ EGIAAIQCGA FHISTGGVTY FNTTPLGRAV TGTMLVAAMK 

       130        140        150        160        170        180 
EDDVNIWGDG STFKGNDIER FYRYGLLTNP NLKIYKPWLD DQFIDELGGR FEMSQFLIAN 

       190        200        210        220        230        240 
GFDYKMSVEK AYSTDSNMLG ATHEAKDLED LSTGIKIVKP IMGVAFWDES VEIKPEVVTV 

       250        260        270        280        290        300 
RFEEGVPVEL NGKRFDDVVE LFMEANRIGG RHGLGMSDQI ENRIIEAKSR GIYEAPGMAL 

       310        320        330        340        350        360 
FHIAYERLVT GIHNEDTIEQ YRINGLRLGR LLYQGRWFDP QALMLRESSQ RWVAKAITGE 

       370        380        390        400        410        420 
VKLELRRGND YSILDTVSPN LTYEAERLSM EKVEDAPFDP IDRIGQLTMR NLDVTDTRNK 

       430        440 
LGIYSEAGLL TAGKDAVVPQ LGSK 

« Hide

References

[1]"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MBEL55E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016827 Genomic DNA. Translation: AAU38086.1.
RefSeqYP_088671.1. NC_006300.1.

3D structure databases

ProteinModelPortalQ65SH4.
SMRQ65SH4. Positions 4-441.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING221988.MS1479.

Proteomic databases

PRIDEQ65SH4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU38086; AAU38086; MS1479.
GeneID3076838.
KEGGmsu:MS1479.
PATRIC22446247. VBIManSuc86752_1346.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230094.
KOK01940.
OMAMRNLDIA.
OrthoDBEOG6K9QCV.
ProtClustDBPRK05370.

Enzyme and pathway databases

BioCycBMAN221988:GHGM-1522-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00581. Arg_succ_synth_type2.
InterProIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_MANSM
AccessionPrimary (citable) accession number: Q65SH4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 25, 2004
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways