Q65SC6 (NAGK_MANSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyl-D-glucosamine kinase
EC=2.7.1.59

Alternative name(s):
GlcNAc kinase

Gene names

Name:	nagK
Ordered Locus Names:	MS1527

Organism

Mannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]

Taxonomic identifier

221988 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Basfia › Mannheimia succiniciproducens

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P By similarity. HAMAP-Rule MF_01271
Catalytic activity	ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate. HAMAP-Rule MF_01271
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_01271
Sequence similarities	Belongs to the ROK (NagC/XylR) family. NagK subfamily.
Sequence caution	The sequence AAU38134.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: HAMAP peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP N-acetylglucosamine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	N-acetyl-D-glucosamine kinase HAMAP-Rule MF_01271		PRO_0000270108
Regions
	Nucleotide binding	4 – 11	8	ATP Potential
	Nucleotide binding	133 – 140	8	ATP Potential
Sites
	Metal binding	157	1	Zinc By similarity
	Metal binding	178	1	Zinc By similarity
	Metal binding	180	1	Zinc By similarity
	Metal binding	185	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65SC6 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: 51503279D504A2B5	FASTA	304	33,284
	10 20 30 40 50 60 MLYGFDIGGT KIELAVFNDK LERQYTERVE TPKDSYEQWL DVIVNLVEKA DQKFACKGSV 70 80 90 100 110 120 GLGLPGFVNH ETGIAEITNI RVADNKPIIK DLSERLGREV RAENDANCFA LSEAWDEENQ 130 140 150 160 170 180 QYPFVLGLIL GTGFGGGLIF NGKVHSGQIG MAGELGHLQL NYHALKLLGW DKAPIYDCGC 190 200 210 220 230 240 GNRACLDTYL SGRGFEMLYR DLKGEALSAK EIIERFYAAD KTAVDFVGLF IELCAISLGN 250 260 270 280 290 300 IITALDPHVI VLGGGLSNFD YLYEALPKAL PKHLMRSAKV PVIKKAKYGD SGGVRGAAAL FLTK

References

[1]

"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens." Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J. Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MBEL55E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016827 Genomic DNA. Translation: AAU38134.1. Different initiation.
RefSeq	YP_088719.2. NC_006300.1.
3D structure databases
HSSP	HSSP built from PDB template 2AP1 based on UniProtKB Q8ZPZ9.
ProteinModelPortal	Q65SC6.
SMR	Q65SC6. Positions 1-303.
ModBase	Search...
Protein-protein interaction databases
STRING	221988.MS1527.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU38134; AAU38134; MS1527.
GeneID	3077056.
KEGG	msu:MS1527.
PATRIC	22446341. VBIManSuc86752_1391.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1940.
HOGENOM	HOG000150087.
KO	K00884.
ProtClustDB	PRK13310.
Enzyme and pathway databases
BioCyc	BMAN221988:GHGM-1521-MONOMER.
UniPathway	UPA00544.
Family and domain databases
HAMAP	MF_01271. GlcNAc_kinase.
InterPro	IPR023505. N-acetyl-D-glucosamine_kinase. IPR000600. ROK. [Graphical view]
Pfam	PF00480. ROK. 1 hit. [Graphical view]
PROSITE	PS01125. ROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGK_MANSM

Accession

Primary (citable) accession number: Q65SC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program