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Q65RY9 (DDL_MANSM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:MS1664
OrganismMannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]
Taxonomic identifier221988 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 307307D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177840

Regions

Domain105 – 304200ATP-grasp
Nucleotide binding135 – 19056ATP By similarity

Sites

Metal binding2581Magnesium or manganese 1 By similarity
Metal binding2711Magnesium or manganese 1 By similarity
Metal binding2711Magnesium or manganese 2 By similarity
Metal binding2731Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65RY9 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 77190C9FCB85441B

FASTA30733,760
        10         20         30         40         50         60 
MKPLKEQKIA VLLGGTSAER EVSLTSGDAV LTALRNQGYD AHPIDPKEYP VAQLKEQGFE 

        70         80         90        100        110        120 
RAFNILHGRG GEDGVIQGVL EQIGLPYTGC GVMTSALTMD KMRTKMLWKG FGLPIADMEI 

       130        140        150        160        170        180 
VTRDTVDELN PLEVVERLGL PLMVKPSREG SSVGLTKVNA VEELKNAVDL ALTHDDTVLI 

       190        200        210        220        230        240 
EEWLSGIEMT VPVLDDQVLP AVQIIPEGEF YDYDAKYISD NTRYICPAPM SEESLQELQK 

       250        260        270        280        290        300 
LVKRAYDVVG CRGWSRIDVM TDANGNFRLV EVNTTPGMTS HSLFPKSAAT VGYSFEQLVV 


KILELSA 

« Hide

References

[1]"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MBEL55E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016827 Genomic DNA. Translation: AAU38271.1.
RefSeqYP_088856.1. NC_006300.1.

3D structure databases

ProteinModelPortalQ65RY9.
SMRQ65RY9. Positions 7-306.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING221988.MS1664.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU38271; AAU38271; MS1664.
GeneID3075094.
KEGGmsu:MS1664.
PATRIC22446603. VBIManSuc86752_1516.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycBMAN221988:GHGM-1714-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_MANSM
AccessionPrimary (citable) accession number: Q65RY9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 25, 2004
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways