SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q65RX0

- GSHAB_MANSM

UniProt

Q65RX0 - GSHAB_MANSM

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Glutathione biosynthesis bifunctional protein GshAB
Gene
gshAB, gshF, MS1683
Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi702 – 7021Magnesium or manganese 1 By similarity
Metal bindingi723 – 7231Magnesium or manganese 1 By similarity
Metal bindingi723 – 7231Magnesium or manganese 2 By similarity
Metal bindingi725 – 7251Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi521 – 58060ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBMAN221988:GHGM-1733-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshAB
Alternative name(s):
Gamma-GCS-GS
Short name:
GCS-GS
Including the following 2 domains:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Glutathione synthetase (EC:6.3.2.3)
Alternative name(s):
GSH synthetase
Short name:
GS
Short name:
GSH-S
Short name:
GSHase
Glutathione synthase
Gene namesi
Name:gshAB
Synonyms:gshF
Ordered Locus Names:MS1683
OrganismiMannheimia succiniciproducens (strain MBEL55E)
Taxonomic identifieri221988 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
ProteomesiUP000000607: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757Glutathione biosynthesis bifunctional protein GshABUniRule annotation
PRO_0000192556Add
BLAST

Proteomic databases

PRIDEiQ65RX0.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi221988.MS1683.

Structurei

3D structure databases

ProteinModelPortaliQ65RX0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini494 – 757264ATP-grasp
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 337337Glutamate--cysteine ligaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OrthoDBiEOG6BKJ7H.

Family and domain databases

Gene3Di3.30.470.20. 2 hits.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65RX0-1 [UniParc]FASTAAdd to Basket

« Hide

MNIQQIVKEK GLGLLFRQGT VGIEKESQRV HADGSIVTSE HPKAFGNRSY    50
HPYIQTDFAE SQLELITPPN KKIEDTLRWL SALHEVTLRT IDENEYIFPM 100
SMPAGLPPEQ EIRVAQLDNA ADVAYREHLV ASYGKAKQMV SGIHYNFQLD 150
PKLVETLFNA QTDYKSAVDF QNNLYLKMAK NFLRYQWIPL YLLSATPTVE 200
ANYFKDGSPL KPNQYVRSLR SSKYGYVNAP DIIVSFDSIE KYVETLEHWV 250
NSGRLIAEKE FYSNVRLRGA KKAREFLHTG IQYLEFRLFD LNPFEAYGIN 300
LKDAKFIHHF ILLMIWLEET ADQDAVELGR ARLGEVAFED PHSETAYRDE 350
GEQIINQLID MLKAIGAEQS AVEFAEEKLA QFANPGQTLC ARLVDAIEQA 400
GGYQQLGGEI AKRNKVQAFE RFYALSAFDN MELSTQALMF DAIQKGLNME 450
ILDENDQFLR LQFGDHFEYV KNGNMTSHDS YISPLIMENK VVTKKVLAKA 500
GFNVPQSLEF TSVEQAVASY PLFEGKAVVI KPKSTNFGLG ISIFQQGVHD 550
KADFAKAVEI AFREDKEVMV EDYLVGTEYR FFVLGNETLA VLLRVPANVM 600
GDGVHTVAEL VAAKNDHPLR GDGSRTPLKK IALGEIEQLQ LKEQGLTVDS 650
VPAKDQLVQL RANSNISTGG DSIDMTDEMH PSYKDLAVGI TKAMGAAVCG 700
VDLIIPDLKK PAEPNLSSWG VIEANFNPMM MMHIFPYSGK SRRLTLNVLG 750
MLFPELV 757
Length:757
Mass (Da):85,101
Last modified:July 5, 2005 - v2
Checksum:iDCCBAE68513F5980
GO

Sequence cautioni

The sequence AAU38290.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016827 Genomic DNA. Translation: AAU38290.1. Different initiation.
RefSeqiYP_088875.1. NC_006300.1.

Genome annotation databases

EnsemblBacteriaiAAU38290; AAU38290; MS1683.
GeneIDi3075060.
KEGGimsu:MS1683.
PATRICi22446639. VBIManSuc86752_1534.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016827 Genomic DNA. Translation: AAU38290.1 . Different initiation.
RefSeqi YP_088875.1. NC_006300.1.

3D structure databases

ProteinModelPortali Q65RX0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 221988.MS1683.

Proteomic databases

PRIDEi Q65RX0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU38290 ; AAU38290 ; MS1683 .
GeneIDi 3075060.
KEGGi msu:MS1683.
PATRICi 22446639. VBIManSuc86752_1534.

Phylogenomic databases

eggNOGi COG1181.
HOGENOMi HOG000156471.
KOi K01919.
OrthoDBi EOG6BKJ7H.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00209 .
UPA00142 ; UER00210 .
BioCyci BMAN221988:GHGM-1733-MONOMER.

Family and domain databases

Gene3Di 3.30.470.20. 2 hits.
HAMAPi MF_00782. Glut_biosynth.
InterProi IPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view ]
Pfami PF04262. Glu_cys_ligase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
    Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
    Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MBEL55E.

Entry informationi

Entry nameiGSHAB_MANSM
AccessioniPrimary (citable) accession number: Q65RX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: May 14, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi