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Q65RX0

- GSHAB_MANSM

UniProt

Q65RX0 - GSHAB_MANSM

Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 2 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi702 – 7021Magnesium or manganese 1UniRule annotation
    Metal bindingi723 – 7231Magnesium or manganese 1UniRule annotation
    Metal bindingi723 – 7231Magnesium or manganese 2UniRule annotation
    Metal bindingi725 – 7251Magnesium or manganese 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi521 – 58060ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
    3. glutathione synthase activity Source: UniProtKB-HAMAP
    4. magnesium ion binding Source: UniProtKB-HAMAP
    5. manganese ion binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBMAN221988:GHGM-1733-MONOMER.
    UniPathwayiUPA00142; UER00209.
    UPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione biosynthesis bifunctional protein GshABUniRule annotation
    Alternative name(s):
    Gamma-GCS-GSUniRule annotation
    Short name:
    GCS-GSUniRule annotation
    Including the following 2 domains:
    Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
    Alternative name(s):
    Gamma-ECSUniRule annotation
    Short name:
    GCSUniRule annotation
    Gamma-glutamylcysteine synthetaseUniRule annotation
    Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
    Alternative name(s):
    GSH synthetaseUniRule annotation
    Short name:
    GSUniRule annotation
    Short name:
    GSH-SUniRule annotation
    Short name:
    GSHaseUniRule annotation
    Glutathione synthaseUniRule annotation
    Gene namesi
    Name:gshABUniRule annotation
    Synonyms:gshFUniRule annotation
    Ordered Locus Names:MS1683
    OrganismiMannheimia succiniciproducens (strain MBEL55E)
    Taxonomic identifieri221988 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
    ProteomesiUP000000607: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 757757Glutathione biosynthesis bifunctional protein GshABPRO_0000192556Add
    BLAST

    Proteomic databases

    PRIDEiQ65RX0.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi221988.MS1683.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65RX0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini494 – 757264ATP-graspUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 337337Glutamate--cysteine ligaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1181.
    HOGENOMiHOG000156471.
    KOiK01919.
    OrthoDBiEOG6BKJ7H.

    Family and domain databases

    Gene3Di3.30.470.20. 2 hits.
    HAMAPiMF_00782. Glut_biosynth.
    InterProiIPR011761. ATP-grasp.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view]
    PfamiPF04262. Glu_cys_ligase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q65RX0-1 [UniParc]FASTAAdd to Basket

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    MNIQQIVKEK GLGLLFRQGT VGIEKESQRV HADGSIVTSE HPKAFGNRSY    50
    HPYIQTDFAE SQLELITPPN KKIEDTLRWL SALHEVTLRT IDENEYIFPM 100
    SMPAGLPPEQ EIRVAQLDNA ADVAYREHLV ASYGKAKQMV SGIHYNFQLD 150
    PKLVETLFNA QTDYKSAVDF QNNLYLKMAK NFLRYQWIPL YLLSATPTVE 200
    ANYFKDGSPL KPNQYVRSLR SSKYGYVNAP DIIVSFDSIE KYVETLEHWV 250
    NSGRLIAEKE FYSNVRLRGA KKAREFLHTG IQYLEFRLFD LNPFEAYGIN 300
    LKDAKFIHHF ILLMIWLEET ADQDAVELGR ARLGEVAFED PHSETAYRDE 350
    GEQIINQLID MLKAIGAEQS AVEFAEEKLA QFANPGQTLC ARLVDAIEQA 400
    GGYQQLGGEI AKRNKVQAFE RFYALSAFDN MELSTQALMF DAIQKGLNME 450
    ILDENDQFLR LQFGDHFEYV KNGNMTSHDS YISPLIMENK VVTKKVLAKA 500
    GFNVPQSLEF TSVEQAVASY PLFEGKAVVI KPKSTNFGLG ISIFQQGVHD 550
    KADFAKAVEI AFREDKEVMV EDYLVGTEYR FFVLGNETLA VLLRVPANVM 600
    GDGVHTVAEL VAAKNDHPLR GDGSRTPLKK IALGEIEQLQ LKEQGLTVDS 650
    VPAKDQLVQL RANSNISTGG DSIDMTDEMH PSYKDLAVGI TKAMGAAVCG 700
    VDLIIPDLKK PAEPNLSSWG VIEANFNPMM MMHIFPYSGK SRRLTLNVLG 750
    MLFPELV 757
    Length:757
    Mass (Da):85,101
    Last modified:July 5, 2005 - v2
    Checksum:iDCCBAE68513F5980
    GO

    Sequence cautioni

    The sequence AAU38290.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016827 Genomic DNA. Translation: AAU38290.1. Different initiation.
    RefSeqiYP_088875.1. NC_006300.1.

    Genome annotation databases

    EnsemblBacteriaiAAU38290; AAU38290; MS1683.
    GeneIDi3075060.
    KEGGimsu:MS1683.
    PATRICi22446639. VBIManSuc86752_1534.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016827 Genomic DNA. Translation: AAU38290.1 . Different initiation.
    RefSeqi YP_088875.1. NC_006300.1.

    3D structure databases

    ProteinModelPortali Q65RX0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 221988.MS1683.

    Proteomic databases

    PRIDEi Q65RX0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU38290 ; AAU38290 ; MS1683 .
    GeneIDi 3075060.
    KEGGi msu:MS1683.
    PATRICi 22446639. VBIManSuc86752_1534.

    Phylogenomic databases

    eggNOGi COG1181.
    HOGENOMi HOG000156471.
    KOi K01919.
    OrthoDBi EOG6BKJ7H.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00209 .
    UPA00142 ; UER00210 .
    BioCyci BMAN221988:GHGM-1733-MONOMER.

    Family and domain databases

    Gene3Di 3.30.470.20. 2 hits.
    HAMAPi MF_00782. Glut_biosynth.
    InterProi IPR011761. ATP-grasp.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view ]
    Pfami PF04262. Glu_cys_ligase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
      Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
      Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MBEL55E.

    Entry informationi

    Entry nameiGSHAB_MANSM
    AccessioniPrimary (citable) accession number: Q65RX0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 59 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3