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Q65RX0

- GSHAB_MANSM

UniProt

Q65RX0 - GSHAB_MANSM

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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi702 – 7021Magnesium or manganese 1UniRule annotation
Metal bindingi723 – 7231Magnesium or manganese 1UniRule annotation
Metal bindingi723 – 7231Magnesium or manganese 2UniRule annotation
Metal bindingi725 – 7251Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi521 – 58060ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBMAN221988:GHGM-1733-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshABUniRule annotation
Alternative name(s):
Gamma-GCS-GSUniRule annotation
Short name:
GCS-GSUniRule annotation
Including the following 2 domains:
Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-ECSUniRule annotation
Short name:
GCSUniRule annotation
Gamma-glutamylcysteine synthetaseUniRule annotation
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshABUniRule annotation
Synonyms:gshFUniRule annotation
Ordered Locus Names:MS1683
OrganismiMannheimia succiniciproducens (strain MBEL55E)
Taxonomic identifieri221988 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
ProteomesiUP000000607: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757Glutathione biosynthesis bifunctional protein GshABPRO_0000192556Add
BLAST

Proteomic databases

PRIDEiQ65RX0.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi221988.MS1683.

Structurei

3D structure databases

ProteinModelPortaliQ65RX0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini494 – 757264ATP-graspUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 337337Glutamate--cysteine ligaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OrthoDBiEOG6BKJ7H.

Family and domain databases

Gene3Di3.30.470.20. 2 hits.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65RX0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNIQQIVKEK GLGLLFRQGT VGIEKESQRV HADGSIVTSE HPKAFGNRSY
60 70 80 90 100
HPYIQTDFAE SQLELITPPN KKIEDTLRWL SALHEVTLRT IDENEYIFPM
110 120 130 140 150
SMPAGLPPEQ EIRVAQLDNA ADVAYREHLV ASYGKAKQMV SGIHYNFQLD
160 170 180 190 200
PKLVETLFNA QTDYKSAVDF QNNLYLKMAK NFLRYQWIPL YLLSATPTVE
210 220 230 240 250
ANYFKDGSPL KPNQYVRSLR SSKYGYVNAP DIIVSFDSIE KYVETLEHWV
260 270 280 290 300
NSGRLIAEKE FYSNVRLRGA KKAREFLHTG IQYLEFRLFD LNPFEAYGIN
310 320 330 340 350
LKDAKFIHHF ILLMIWLEET ADQDAVELGR ARLGEVAFED PHSETAYRDE
360 370 380 390 400
GEQIINQLID MLKAIGAEQS AVEFAEEKLA QFANPGQTLC ARLVDAIEQA
410 420 430 440 450
GGYQQLGGEI AKRNKVQAFE RFYALSAFDN MELSTQALMF DAIQKGLNME
460 470 480 490 500
ILDENDQFLR LQFGDHFEYV KNGNMTSHDS YISPLIMENK VVTKKVLAKA
510 520 530 540 550
GFNVPQSLEF TSVEQAVASY PLFEGKAVVI KPKSTNFGLG ISIFQQGVHD
560 570 580 590 600
KADFAKAVEI AFREDKEVMV EDYLVGTEYR FFVLGNETLA VLLRVPANVM
610 620 630 640 650
GDGVHTVAEL VAAKNDHPLR GDGSRTPLKK IALGEIEQLQ LKEQGLTVDS
660 670 680 690 700
VPAKDQLVQL RANSNISTGG DSIDMTDEMH PSYKDLAVGI TKAMGAAVCG
710 720 730 740 750
VDLIIPDLKK PAEPNLSSWG VIEANFNPMM MMHIFPYSGK SRRLTLNVLG

MLFPELV
Length:757
Mass (Da):85,101
Last modified:July 5, 2005 - v2
Checksum:iDCCBAE68513F5980
GO

Sequence cautioni

The sequence AAU38290.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016827 Genomic DNA. Translation: AAU38290.1. Different initiation.
RefSeqiYP_088875.1. NC_006300.1.

Genome annotation databases

EnsemblBacteriaiAAU38290; AAU38290; MS1683.
GeneIDi3075060.
KEGGimsu:MS1683.
PATRICi22446639. VBIManSuc86752_1534.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016827 Genomic DNA. Translation: AAU38290.1 . Different initiation.
RefSeqi YP_088875.1. NC_006300.1.

3D structure databases

ProteinModelPortali Q65RX0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 221988.MS1683.

Proteomic databases

PRIDEi Q65RX0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU38290 ; AAU38290 ; MS1683 .
GeneIDi 3075060.
KEGGi msu:MS1683.
PATRICi 22446639. VBIManSuc86752_1534.

Phylogenomic databases

eggNOGi COG1181.
HOGENOMi HOG000156471.
KOi K01919.
OrthoDBi EOG6BKJ7H.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00209 .
UPA00142 ; UER00210 .
BioCyci BMAN221988:GHGM-1733-MONOMER.

Family and domain databases

Gene3Di 3.30.470.20. 2 hits.
HAMAPi MF_00782. Glut_biosynth.
InterProi IPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view ]
Pfami PF04262. Glu_cys_ligase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
    Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
    Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MBEL55E.

Entry informationi

Entry nameiGSHAB_MANSM
AccessioniPrimary (citable) accession number: Q65RX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: November 26, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3