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Q65RX0 (GSHAB_MANSM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione biosynthesis bifunctional protein GshAB
Alternative name(s):
Gamma-GCS-GS
Short name=GCS-GS

Including the following 2 domains:

  1. Glutamate--cysteine ligase
    EC=6.3.2.2
    Alternative name(s):
    Gamma-ECS
    Short name=GCS
    Gamma-glutamylcysteine synthetase
  2. Glutathione synthetase
    EC=6.3.2.3
    Alternative name(s):
    GSH synthetase
    Short name=GS
    Short name=GSH-S
    Short name=GSHase
    Glutathione synthase
Gene names
Name:gshAB
Synonyms:gshF
Ordered Locus Names:MS1683
OrganismMannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]
Taxonomic identifier221988 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia

Protein attributes

Sequence length757 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine By similarity. HAMAP-Rule MF_00782

Catalytic activity

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP-Rule MF_00782

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00782

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP-Rule MF_00782

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00782

Sequence similarities

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence AAU38290.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 757757Glutathione biosynthesis bifunctional protein GshAB HAMAP-Rule MF_00782
PRO_0000192556

Regions

Domain494 – 757264ATP-grasp
Nucleotide binding521 – 58060ATP By similarity
Region1 – 337337Glutamate--cysteine ligase HAMAP-Rule MF_00782

Sites

Metal binding7021Magnesium or manganese 1 By similarity
Metal binding7231Magnesium or manganese 1 By similarity
Metal binding7231Magnesium or manganese 2 By similarity
Metal binding7251Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65RX0 [UniParc].

Last modified July 5, 2005. Version 2.
Checksum: DCCBAE68513F5980

FASTA75785,101
        10         20         30         40         50         60 
MNIQQIVKEK GLGLLFRQGT VGIEKESQRV HADGSIVTSE HPKAFGNRSY HPYIQTDFAE 

        70         80         90        100        110        120 
SQLELITPPN KKIEDTLRWL SALHEVTLRT IDENEYIFPM SMPAGLPPEQ EIRVAQLDNA 

       130        140        150        160        170        180 
ADVAYREHLV ASYGKAKQMV SGIHYNFQLD PKLVETLFNA QTDYKSAVDF QNNLYLKMAK 

       190        200        210        220        230        240 
NFLRYQWIPL YLLSATPTVE ANYFKDGSPL KPNQYVRSLR SSKYGYVNAP DIIVSFDSIE 

       250        260        270        280        290        300 
KYVETLEHWV NSGRLIAEKE FYSNVRLRGA KKAREFLHTG IQYLEFRLFD LNPFEAYGIN 

       310        320        330        340        350        360 
LKDAKFIHHF ILLMIWLEET ADQDAVELGR ARLGEVAFED PHSETAYRDE GEQIINQLID 

       370        380        390        400        410        420 
MLKAIGAEQS AVEFAEEKLA QFANPGQTLC ARLVDAIEQA GGYQQLGGEI AKRNKVQAFE 

       430        440        450        460        470        480 
RFYALSAFDN MELSTQALMF DAIQKGLNME ILDENDQFLR LQFGDHFEYV KNGNMTSHDS 

       490        500        510        520        530        540 
YISPLIMENK VVTKKVLAKA GFNVPQSLEF TSVEQAVASY PLFEGKAVVI KPKSTNFGLG 

       550        560        570        580        590        600 
ISIFQQGVHD KADFAKAVEI AFREDKEVMV EDYLVGTEYR FFVLGNETLA VLLRVPANVM 

       610        620        630        640        650        660 
GDGVHTVAEL VAAKNDHPLR GDGSRTPLKK IALGEIEQLQ LKEQGLTVDS VPAKDQLVQL 

       670        680        690        700        710        720 
RANSNISTGG DSIDMTDEMH PSYKDLAVGI TKAMGAAVCG VDLIIPDLKK PAEPNLSSWG 

       730        740        750 
VIEANFNPMM MMHIFPYSGK SRRLTLNVLG MLFPELV 

« Hide

References

[1]"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MBEL55E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016827 Genomic DNA. Translation: AAU38290.1. Different initiation.
RefSeqYP_088875.1. NC_006300.1.

3D structure databases

ProteinModelPortalQ65RX0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING221988.MS1683.

Proteomic databases

PRIDEQ65RX0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU38290; AAU38290; MS1683.
GeneID3075060.
KEGGmsu:MS1683.
PATRIC22446639. VBIManSuc86752_1534.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000156471.
KOK01919.
OrthoDBEOG6BKJ7H.

Enzyme and pathway databases

BioCycBMAN221988:GHGM-1733-MONOMER.
UniPathwayUPA00142; UER00209.
UPA00142; UER00210.

Family and domain databases

Gene3D3.30.470.20. 2 hits.
HAMAPMF_00782. Glut_biosynth.
InterProIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHAB_MANSM
AccessionPrimary (citable) accession number: Q65RX0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: May 14, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways