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Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Gene

ispH

Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).UniRule annotation

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation

Cofactori

[3Fe-4S] clusterUniRule annotationNote: Binds 1 [3Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121Iron-sulfur (3Fe-4S)UniRule annotation
Binding sitei41 – 411SubstrateUniRule annotation
Binding sitei74 – 741SubstrateUniRule annotation
Metal bindingi96 – 961Iron-sulfur (3Fe-4S)UniRule annotation
Binding sitei124 – 1241SubstrateUniRule annotation
Binding sitei167 – 1671SubstrateUniRule annotation
Metal bindingi197 – 1971Iron-sulfur (3Fe-4S)UniRule annotation
Binding sitei269 – 2691SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

3Fe-4S, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciBMAN221988:GHGM-1802-MONOMER.
UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation (EC:1.17.7.4UniRule annotation)
Gene namesi
Name:ispHUniRule annotation
Synonyms:lytB
Ordered Locus Names:MS1749
OrganismiMannheimia succiniciproducens (strain MBEL55E)
Taxonomic identifieri221988 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
Proteomesi
  • UP000000607 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3143144-hydroxy-3-methylbut-2-enyl diphosphate reductasePRO_0000128834Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi221988.MS1749.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni225 – 2273Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the IspH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C48. Bacteria.
COG0761. LUCA.
HOGENOMiHOG000220192.
KOiK03527.
OMAiHTIDATC.
OrthoDBiPOG091H038O.

Family and domain databases

CDDicd13944. lytB_ispH. 1 hit.
HAMAPiMF_00191. IspH. 1 hit.
InterProiIPR003451. LytB/IspH.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q65RQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIILANPRG FCAGVDRAIS IVELALEIHG APIYVRHEVV HNRFVVNGLR
60 70 80 90 100
ERGAVFVEEL NEVPDGAIVI FSAHGVSQAV RQEAKNRNLK VFDATCPLVT
110 120 130 140 150
KVHMQVARAS RKGTKAILIG HEGHPEVQGT MGQYDNPEGG IFLVENVEDI
160 170 180 190 200
AKLGLKDNEE LTFMTQTTLS IDDTSDVIVA LKAKYPAIQG PRKNDICYAT
210 220 230 240 250
TNRQQAVREL AEQSDLVIVV GSKNSSNSNR LAELASRMGV PAKLIDDSND
260 270 280 290 300
IEPDWLKGIN TIGVTAGASA PEVLVQSVIA RLKELGVDSV EELEGCEENT
310
VFEVPKELRI KEVG
Length:314
Mass (Da):34,166
Last modified:October 25, 2004 - v1
Checksum:i7C4CBFE487322ED7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016827 Genomic DNA. Translation: AAU38356.1.
RefSeqiWP_011200915.1. NC_006300.1.

Genome annotation databases

EnsemblBacteriaiAAU38356; AAU38356; MS1749.
KEGGimsu:MS1749.
PATRICi22446771. VBIManSuc86752_1595.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016827 Genomic DNA. Translation: AAU38356.1.
RefSeqiWP_011200915.1. NC_006300.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi221988.MS1749.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU38356; AAU38356; MS1749.
KEGGimsu:MS1749.
PATRICi22446771. VBIManSuc86752_1595.

Phylogenomic databases

eggNOGiENOG4105C48. Bacteria.
COG0761. LUCA.
HOGENOMiHOG000220192.
KOiK03527.
OMAiHTIDATC.
OrthoDBiPOG091H038O.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.
BioCyciBMAN221988:GHGM-1802-MONOMER.

Family and domain databases

CDDicd13944. lytB_ispH. 1 hit.
HAMAPiMF_00191. IspH. 1 hit.
InterProiIPR003451. LytB/IspH.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiISPH_MANSM
AccessioniPrimary (citable) accession number: Q65RQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: October 25, 2004
Last modified: September 7, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.