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Q65RQ2 (SYI_MANSM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:MS1751
OrganismMannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]
Taxonomic identifier221988 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia

Protein attributes

Sequence length938 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 938938Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098413

Regions

Motif65 – 7511"HIGH" region HAMAP-Rule MF_02002
Motif609 – 6135"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding9051Zinc By similarity
Metal binding9081Zinc By similarity
Metal binding9211Zinc By similarity
Metal binding9241Zinc By similarity
Binding site5681Aminoacyl-adenylate By similarity
Binding site6121ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65RQ2 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 6BB437558466496E

FASTA938106,132
        10         20         30         40         50         60 
MVRKMSEQKD YKNTLNLPET GFPMRGDLAK REPGMLKNWY DNDLYQKIRQ SSKGKKSFIL 

        70         80         90        100        110        120 
HDGPPYANGS IHIGHAVNKI LKDIIIKSKT ALGFDSPYIP GWDCHGLPIE LKVEGLVGKP 

       130        140        150        160        170        180 
NQKISAAQFR EECRKYAREQ VEGQKKDFIR LGVLGDWDNP YLTMNFDTEA NIIRAFGKAV 

       190        200        210        220        230        240 
ANGHLYKGSK PVHWCLDCAS SLAEAEVEYE DRTSPSIYVR FAAADESAVE NKFVLTEQGK 

       250        260        270        280        290        300 
GKLSAVIWTT TPWTLPSNKA ISINPELEYQ IVQFGDERFI LAAELVESVA QAVGVESWKA 

       310        320        330        340        350        360 
LGSAKGSDLE LLQFKHPFYD YNVPFILGDH VTLDGGTGLV HTAPDHGQDD YVVARKYNIG 

       370        380        390        400        410        420 
MAGLIGNDGK FNSNAKFFAG LGVFEANGKV LEKLDEVGAL LKLEKIRHSY PHCWRHKTPI 

       430        440        450        460        470        480 
IFRATPQWFI GMETQGLRQQ ALSEIKKVRW IPDWGQARIE KMVENRPDWC ISRQRTWGVP 

       490        500        510        520        530        540 
VALFIHKETE QLHPRTLELI EEVAKLVERK GIQAWWDLDA KDLLGDDAAH YSKVPDTLDV 

       550        560        570        580        590        600 
WFDSGSTYYS VVKNRPEFNG KEADMYLEGS DQHRGWFMSS LMLSTATDNK APYKQVLTHG 

       610        620        630        640        650        660 
FTVDGQGRKM SKSIGNIVTP QEVMDKFGGD ILRLWVASTD YTGEMTVSDE ILKRAADSYR 

       670        680        690        700        710        720 
RIRNTARFLL ANLNGFDPKR DLVQAHEMIS LDRWAVDCAF RAQAEIKEAY DNYQFHTVVQ 

       730        740        750        760        770        780 
RLMKFCSVEM GSFYLDIIKD RQYTTKADSL ARRSCQTALW HIAEALVRWM APILSFTADE 

       790        800        810        820        830        840 
IWGYLPGERG EFVFTEEFYD GLFALDVSES LDDAYWQQVI TVRNEVNRVL EQARNDKVIG 

       850        860        870        880        890        900 
GGLEAEVTIF ANDEYSALLN KLGNELRFVT ITSKAEVKTL ADADVAEGEV AGLAIKAIRS 

       910        920        930 
ANHKCPRCWH YSDSKDANSL CSRCEENVNG NGEERRFA 

« Hide

References

[1]"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MBEL55E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016827 Genomic DNA. Translation: AAU38358.1.
RefSeqYP_088943.1. NC_006300.1.

3D structure databases

ProteinModelPortalQ65RQ2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING221988.MS1751.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU38358; AAU38358; MS1751.
GeneID3074830.
KEGGmsu:MS1751.
PATRIC22446775. VBIManSuc86752_1597.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAVLGDWDN.
OrthoDBEOG644ZM1.
ProtClustDBPRK05743.

Enzyme and pathway databases

BioCycBMAN221988:GHGM-1804-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_MANSM
AccessionPrimary (citable) accession number: Q65RQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 25, 2004
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries