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Q65RQ2

- SYI_MANSM

UniProt

Q65RQ2 - SYI_MANSM

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (25 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
    Binding sitei612 – 6121ATPUniRule annotation
    Metal bindingi905 – 9051ZincUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBMAN221988:GHGM-1804-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:MS1751
    OrganismiMannheimia succiniciproducens (strain MBEL55E)
    Taxonomic identifieri221988 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
    ProteomesiUP000000607: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 938938Isoleucine--tRNA ligasePRO_0000098413Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi221988.MS1751.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65RQ2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi65 – 7511"HIGH" regionAdd
    BLAST
    Motifi609 – 6135"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q65RQ2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVRKMSEQKD YKNTLNLPET GFPMRGDLAK REPGMLKNWY DNDLYQKIRQ    50
    SSKGKKSFIL HDGPPYANGS IHIGHAVNKI LKDIIIKSKT ALGFDSPYIP 100
    GWDCHGLPIE LKVEGLVGKP NQKISAAQFR EECRKYAREQ VEGQKKDFIR 150
    LGVLGDWDNP YLTMNFDTEA NIIRAFGKAV ANGHLYKGSK PVHWCLDCAS 200
    SLAEAEVEYE DRTSPSIYVR FAAADESAVE NKFVLTEQGK GKLSAVIWTT 250
    TPWTLPSNKA ISINPELEYQ IVQFGDERFI LAAELVESVA QAVGVESWKA 300
    LGSAKGSDLE LLQFKHPFYD YNVPFILGDH VTLDGGTGLV HTAPDHGQDD 350
    YVVARKYNIG MAGLIGNDGK FNSNAKFFAG LGVFEANGKV LEKLDEVGAL 400
    LKLEKIRHSY PHCWRHKTPI IFRATPQWFI GMETQGLRQQ ALSEIKKVRW 450
    IPDWGQARIE KMVENRPDWC ISRQRTWGVP VALFIHKETE QLHPRTLELI 500
    EEVAKLVERK GIQAWWDLDA KDLLGDDAAH YSKVPDTLDV WFDSGSTYYS 550
    VVKNRPEFNG KEADMYLEGS DQHRGWFMSS LMLSTATDNK APYKQVLTHG 600
    FTVDGQGRKM SKSIGNIVTP QEVMDKFGGD ILRLWVASTD YTGEMTVSDE 650
    ILKRAADSYR RIRNTARFLL ANLNGFDPKR DLVQAHEMIS LDRWAVDCAF 700
    RAQAEIKEAY DNYQFHTVVQ RLMKFCSVEM GSFYLDIIKD RQYTTKADSL 750
    ARRSCQTALW HIAEALVRWM APILSFTADE IWGYLPGERG EFVFTEEFYD 800
    GLFALDVSES LDDAYWQQVI TVRNEVNRVL EQARNDKVIG GGLEAEVTIF 850
    ANDEYSALLN KLGNELRFVT ITSKAEVKTL ADADVAEGEV AGLAIKAIRS 900
    ANHKCPRCWH YSDSKDANSL CSRCEENVNG NGEERRFA 938
    Length:938
    Mass (Da):106,132
    Last modified:October 25, 2004 - v1
    Checksum:i6BB437558466496E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016827 Genomic DNA. Translation: AAU38358.1.
    RefSeqiYP_088943.1. NC_006300.1.

    Genome annotation databases

    EnsemblBacteriaiAAU38358; AAU38358; MS1751.
    GeneIDi3074830.
    KEGGimsu:MS1751.
    PATRICi22446775. VBIManSuc86752_1597.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016827 Genomic DNA. Translation: AAU38358.1 .
    RefSeqi YP_088943.1. NC_006300.1.

    3D structure databases

    ProteinModelPortali Q65RQ2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 221988.MS1751.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU38358 ; AAU38358 ; MS1751 .
    GeneIDi 3074830.
    KEGGi msu:MS1751.
    PATRICi 22446775. VBIManSuc86752_1597.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BMAN221988:GHGM-1804-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
      Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
      Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MBEL55E.

    Entry informationi

    Entry nameiSYI_MANSM
    AccessioniPrimary (citable) accession number: Q65RQ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3