Reviewed, UniProtKB/Swiss-Prot Q65RJ7 (PUR4_MANSM)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	MS1806

Organism

Mannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]

Taxonomic identifier

221988 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Mannheimia

Protein attributes

Sequence length	1297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm. HAMAP MF_00419
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1297	1297	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000264579
Regions
	Domain	1044 – 1297	254	Glutamine amidotransferase type-1
	Nucleotide binding	307 – 318	12	ATP Potential
Sites
	Active site	1137	1	Nucleophile By similarity
	Active site	1262	1	By similarity
	Active site	1264	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65RJ7-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 171374406217BEB4	FASTA	1,297	142,440
	10 20 30 40 50 60 MFQIFRGSPA LSEFRLNQLS ARFQKADLPV KSCYAEYLHF ADLSAGLSAE ETDELEQLLH 70 80 90 100 110 120 YGPTLAQHES KGECFVVIPR VGTISSWSSK ATDIAHNCGL DKVVRLERGI AYYFEFERTL 130 140 150 160 170 180 SAEQQQRLVS HIHDRMMETV VRAPEQAAVL FDSQDPKPFT TVDILNGGRK ALEIANVELG 190 200 210 220 230 240 LALASDEMDY LVENFTALGR NPNDIELYMF AQANSEHCRH KIFNADWVID GEKQEKSLFK 250 260 270 280 290 300 MIKNTFEKTP DHVLSAYKDN AAVMEGSKVG RFFADQDGQY RYHNEDAHIL MKVETHNHPT 310 320 330 340 350 360 AISPFPGAAT GSGGEIRDEG ATGRGAKPKA GLVGFSVSNL VIPGFEQPWE NELSKPSRIS 370 380 390 400 410 420 SALDIMIEGP LGGAAFNNEF GRPALLGYFR TYEEKVNSFA GEEVRGYHKP IMLAGGIGNI 430 440 450 460 470 480 RAEHVQKGEI PVGAKLIVLG GPAMNIGLGG GAASSMTSGK SKEDLDFASV QRDNPEMERR 490 500 510 520 530 540 CQEVIDRCWQ MGEGNPIAFI HDVGAGGLSN AMPELVHDGG RGGKFELRNI LCDERGMSPL 550 560 570 580 590 600 EIWCNESQER YVLAVAPENL AVFEELCQRE RAPYAIIGEA TEEEHLTLHD NHFDNNPIDL 610 620 630 640 650 660 PMSLLLGKTP KMTRDVKSTQ VNNSPVDQTN IELKEAFHRV LRLPVVAEKT FLITIGDRTV 670 680 690 700 710 720 TGMVARDQMV GPWQIPVSDV AVTTAALDTY HGEAMSIGER APVALLDFAA SARLAVAESI 730 740 750 760 770 780 TNIAATNIGD IKRIKLSANW MSAAGHEGED AGLYEAVKAV GEELCPALGL TVPVGKDSMS 790 800 810 820 830 840 MKTTWSENGE QKTVTAPLSL VISAFARVED VRKTVTPQLR TDKGETALLL IDLGEGKNRL 850 860 870 880 890 900 GATALAQVYK QLGDKPADVV NVELLKGFYN AMQTLVQQGK LLAYHDRSDG GLIVTLAEMA 910 920 930 940 950 960 FAGNCGIRAE ISALGDNDLG ILFSEELGAV IQVRESDLAA VREVLTQHGL IHLTKDLGLV 970 980 990 1000 1010 1020 TEYDEFEIKR GTKVVLSEKR SELRGIWAEL THQMQRLRDN PECADQEFAA KKDPANQGFS 1030 1040 1050 1060 1070 1080 AHLTYDINED VAAPYIATGK KPRIAVLREQ GVNSHVEMGA AFDRAGFEAI DVHMSDLHTA 1090 1100 1110 1120 1130 1140 RQNLKDFNAL VACGGFSYGD VLGAGGGWAK SVLFNTALRD QFQAFFERED TLALGVCNGC 1150 1160 1170 1180 1190 1200 QMISTLADII PGTENWPRFV RNTSERFEAR AALVRINESN SVWFQGMAGS HMPIAVSHGE 1210 1220 1230 1240 1250 1260 GRVEFKNDSQ LQGLRDQGLI IAQYVDNNIR PTEVYPANPN GSVDGITALS NTNGRVAIMM 1270 1280 1290 PHPERVFRTV SNSWHPEDWS EDGAWMRLFR NARVFFK

References

[1]

"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens." Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J. Nat. Biotechnol. 22:1275-1281(2004) [PubMed: 15378067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016827 Genomic DNA. Translation: AAU38413.1.
RefSeq	YP_088998.1.
3D structure databases
SMR	Q65RJ7. Positions 1-1293.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65RJ7.
Genome annotation databases
GeneID	3075302.
GenomeReviews	Gene locus MS1806 in contig AE016827_GR.
KEGG	msu:MS1806.
NMPDR	fig|221988.1.peg.1696.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65RJ7.
OMA	ERGIAYY.
Family and domain databases
HAMAP	MF_00419. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_MANSM

Accession

Primary (citable) accession number: Q65RJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)