Reviewed, UniProtKB/Swiss-Prot Q65RI4 (NRFA_MANSM)

Names and origin

Protein names

Recommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase

Gene names

Name:	nrfA
Ordered Locus Names:	MS1819

Organism

Mannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]

Taxonomic identifier

221988 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Mannheimia

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a role in nitrite reduction By similarity.
Catalytic activity	NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+).
Cofactor	Binds 1 calcium ion per monomer By similarity. Binds 5 heme groups covalently per monomer By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation).
Subcellular location	PeriplasmBy similarity.
Sequence similarities	Belongs to the cytochrome c-552 family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrogen compound metabolic process Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: HAMAP
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrite reductase (cytochrome, ammonia-forming) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 525	493	Cytochrome c-552		PRO_0000268968
Sites
	Metal binding	129	1	Iron (heme 3 axial ligand) By similarity
	Metal binding	161	1	Iron (heme 1 axial ligand) By similarity
	Metal binding	199	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	248	1	Iron (heme 3 axial ligand) By similarity
	Metal binding	250	1	Calcium By similarity
	Metal binding	251	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	298	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	300	1	Calcium By similarity
	Metal binding	312	1	Iron (heme 5 axial ligand) By similarity
	Metal binding	323	1	Iron (heme 4 axial ligand) By similarity
	Metal binding	338	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	357	1	Iron (heme 5 axial ligand) By similarity
	Metal binding	432	1	Iron (heme 4 axial ligand) By similarity
	Binding site	157	1	Heme 1 (covalent) By similarity
	Binding site	160	1	Heme 1 (covalent) By similarity
	Binding site	195	1	Heme 2 (covalent) By similarity
	Binding site	198	1	Heme 2 (covalent) By similarity
	Binding site	244	1	Heme 3 (covalent) By similarity
	Binding site	247	1	Heme 3 (covalent) By similarity
	Binding site	251	1	Substrate By similarity
	Binding site	301	1	Substrate By similarity
	Binding site	319	1	Heme 4 (covalent) By similarity
	Binding site	322	1	Heme 4 (covalent) By similarity
	Binding site	353	1	Heme 5 (covalent) By similarity
	Binding site	356	1	Heme 5 (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65RI4-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 747EF08AFB3036BB	FASTA	525	58,466
	10 20 30 40 50 60 MNGVIIVNIL RKTLSSLAIV GLGFAMANSA VAEEKAMATH QQAQQLQQPA PETAAKRAPT 70 80 90 100 110 120 KEELTPVNPN LKIEAANEKF AADFPRQYNS WAKTAEQTEF HKEVEDDPRM IVMWGGYAFA 130 140 150 160 170 180 KEFNSPRGHI YAVTDVRNIL RTGSPKDANG GPQPMACWTC KGPDVPRLIA EWGEEGYFSG 190 200 210 220 230 240 KWAKGGAEVV NSIGCADCHD TQSQDFKDGK PALRVARPHV LRALDTVGKT FATSDRTDQR 250 260 270 280 290 300 AGVCANCHVE YYFDKSTGAN NVVFPWYKGR DVDSIEKYYD EIGFKDWEHS ISKAPMLKAQ 310 320 330 340 350 360 HPDFETWSMG THGKNGVTCV DCHMAKTQDK DGKVYTDHQV VGNPVKDNFQ NTCARCHDQS 370 380 390 400 410 420 QDTLIKTVEQ HKADVREVML KLEDQLVKSH FEAKTAWDNG ATQEEMKDAL QAIRHAQWRW 430 440 450 460 470 480 DFAAASHGMH MHAPDVALKI IASGLDRVAD ARAKLAVILA KHGVQQPIQY PDISTAEKAW 490 500 510 520 KVMGIDIEKE RKEKEEFIKT VIPEWNKEAI SKGLILTAPP TTPAK

References

[1]

"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens." Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J. Nat. Biotechnol. 22:1275-1281(2004) [PubMed: 15378067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016827 Genomic DNA. Translation: AAU38426.1.
RefSeq	YP_089011.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3075137.
GenomeReviews	Gene locus MS1819 in contig AE016827_GR.
KEGG	msu:MS1819.
NMPDR	fig|221988.1.peg.1708.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65RI4.
Family and domain databases
HAMAP	MF_01182. [Tree]
InterPro	IPR003321. Cyt_c552. IPR017570. Cytc_552_NO2Rdtase_formate-dep. IPR011031. Multihaem_cyt. [Graphical view]
Pfam	PF02335. Cytochrom_C552. 1 hit. [Graphical view]
PIRSF	PIRSF000243. Cyt_c552. 1 hit.
PROSITE	PS51008. MULTIHEME_CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NRFA_MANSM

Accession

Primary (citable) accession number: Q65RI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	October 25, 2004
Last modified:	November 4, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)