Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate 5-kinase

Gene

proB

Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14ATPUniRule annotation1
Binding sitei54SubstrateUniRule annotation1
Binding sitei141SubstrateUniRule annotation1
Binding sitei153Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi173 – 174ATPUniRule annotation2
Nucleotide bindingi215 – 221ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:MS1862
OrganismiMannheimia succiniciproducens (strain MBEL55E)
Taxonomic identifieri221988 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
Proteomesi
  • UP000000607 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001096901 – 373Glutamate 5-kinaseAdd BLAST373

Interactioni

Protein-protein interaction databases

STRINGi221988.MS1862.

Structurei

3D structure databases

ProteinModelPortaliQ65RE1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini279 – 357PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246369.
KOiK00931.
OrthoDBiPOG091H00OF.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65RE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQKQNSQKT IVVKFGTSTL TQGTPKLNRA HMIEIVRQLA QLHQEGYRLV
60 70 80 90 100
IVTSGAMAAG RHYLNHPKLP PTIASKQLLA AVGQSQLIQT WEQLFAIYDI
110 120 130 140 150
HIGQLLLTRA DIEDRERFLN ARDTLHALLD NRIIPVVNEN DAVATAEIKV
160 170 180 190 200
GDNDNLSALV AILVQAEQLY LLTDQQGLFD SDPRKNPQAK LIPVVNEITD
210 220 230 240 250
HIRSIAGGSG TTLGTGGMST KITAADIATR SGIETIIAPG NRENVIADLA
260 270 280 290 300
HGEAIGTKFT VQTDKLESRK QWLFAAPSAG ILTIDQGAEN AILEQHKSLL
310 320 330 340 350
PAGIVNIEGR FSRGEVVKIR TQQGKDIALG MPRYNSDALY LIQGKKSQNI
360 370
EKILGYEYGS VAIHRDDMIV LNK
Length:373
Mass (Da):40,841
Last modified:October 17, 2006 - v2
Checksum:iB86EDF1805DAF7FC
GO

Sequence cautioni

The sequence AAU38469 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016827 Genomic DNA. Translation: AAU38469.1. Different initiation.
RefSeqiWP_011201025.1. NC_006300.1.

Genome annotation databases

EnsemblBacteriaiAAU38469; AAU38469; MS1862.
KEGGimsu:MS1862.
PATRICi22446989. VBIManSuc86752_1701.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016827 Genomic DNA. Translation: AAU38469.1. Different initiation.
RefSeqiWP_011201025.1. NC_006300.1.

3D structure databases

ProteinModelPortaliQ65RE1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi221988.MS1862.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU38469; AAU38469; MS1862.
KEGGimsu:MS1862.
PATRICi22446989. VBIManSuc86752_1701.

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246369.
KOiK00931.
OrthoDBiPOG091H00OF.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPROB_MANSM
AccessioniPrimary (citable) accession number: Q65RE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: October 17, 2006
Last modified: November 2, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.