Q65QH0 (GLPE_MANSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiosulfate sulfurtransferase glpE
EC=2.8.1.1

Gene names

Name:	glpE
Synonyms:	pspE
Ordered Locus Names:	MS2183

Organism

Mannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]

Taxonomic identifier

221988 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Basfia

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide By similarity. HAMAP MF_01009
Catalytic activity	Thiosulfate + cyanide = sulfite + thiocyanate. HAMAP MF_01009
Subcellular location	Cytoplasm By similarity HAMAP MF_01009.
Sequence similarities	Belongs to the glpE family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiosulfate sulfurtransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 105	105	Thiosulfate sulfurtransferase glpE HAMAP MF_01009		PRO_0000200555
Regions
	Domain	15 – 103	89	Rhodanese
Sites
	Active site	63	1	Cysteine persulfide intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65QH0 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 536AF592819FC4B9	FASTA	105	11,973
	10 20 30 40 50 60 MFKEITPQQA WQLMIEENAT LVDIRDEQRF TYSHAKGAFH LTGQSYGKFQ IQCDFDDPVI 70 80 90 100 VSCYHGISSR NVAAFLVEQG YDNIYSIIGG FEGWQRAGLP IETAY

References

[1]

"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens." Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J. Nat. Biotechnol. 22:1275-1281(2004) [PubMed: 15378067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MBEL55E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016827 Genomic DNA. Translation: AAU38790.1.
RefSeq	YP_089375.1. NC_006300.1.
3D structure databases
ProteinModelPortal	Q65QH0.
SMR	Q65QH0. Positions 2-105.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65QH0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3076149.
GenomeReviews	Gene locus MS2183 in contig AE016827_GR.
KEGG	msu:MS2183.
NMPDR	fig|221988.1.peg.2056.
PATRIC	22447581. VBIManSuc86752_1996.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0607.
HOGENOM	HBG743107.
OMA	FSAWHEA.
PhylomeDB	Q65QH0.
ProtClustDB	CLSK892378.
Enzyme and pathway databases
BioCyc	MSUC221988:MS2183-MONOMER.
Family and domain databases
HAMAP	MF_01009. Thiosulf_sulfurtr. [Tree]
InterPro	IPR001763. Rhodanese-like. IPR023695. Thiosulf_sulfurTrfase. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 1 hit.
KO	K02439.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPE_MANSM

Accession

Primary (citable) accession number: Q65QH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program