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Q65Q72

- NAPA_MANSM

UniProt

Q65Q72 - NAPA_MANSM

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Protein

Periplasmic nitrate reductase

Gene
napA, MS2281
Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S) By similarity
Metal bindingi49 – 491Iron-sulfur (4Fe-4S) By similarity
Metal bindingi53 – 531Iron-sulfur (4Fe-4S) By similarity
Metal bindingi81 – 811Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciBMAN221988:GHGM-2340-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:MS2281
OrganismiMannheimia succiniciproducens (strain MBEL55E)
Taxonomic identifieri221988 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
ProteomesiUP000000607: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signal Reviewed predictionAdd
BLAST
Chaini33 – 830798Periplasmic nitrate reductaseUniRule annotationPRO_0000045991Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi221988.MS2281.

Structurei

3D structure databases

ProteinModelPortaliQ65Q72.
SMRiQ65Q72. Positions 38-829.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q65Q72-1 [UniParc]FASTAAdd to Basket

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MELNRRDFMK ANAAVAAAAA AGITIPVKNV HAADDDMGIR WDKAPCRYCG    50
TGCSVLVGTK DGRVVATQGD PDAEVNRGLN CIKGYFLSKI MYGADRVQTP 100
LLRMKDGKFH KEGDFTPVSW DQAFTVMADK IKAILKEKKD PNAIGMFSSG 150
QTTIFEGYAK VKLWKAGLRS NTIDPNARHC MASAAVAFLR TFGMDEPMGC 200
YNDIEKTDAF VLWGSNMAEM HPILWSRISD RRLSSDKVKV VVMSTFEHRS 250
FELADTPIIF KPHSDLAILN YIANYIIQND KVNWDFVNKH TKFKRGETDI 300
GYGLRPEHPL EVAAKNRKTA GKMYDSDFEE FKKIVAPYTL EEAHRISGVP 350
KDQLETLAKM YADPQQNLVS FWTMGFNQHT RGVWVNHMVY NVHLLTGKIS 400
KPGCGPFSLT GQPSACGTAR EVGTFVHRLP ADMVVTNPKH VEIAENIWKL 450
PKGTISNKPG FPAVQQSRAL KDGKLNFLWQ LCTNNMQGGP NINEEIFPGW 500
RNPDNLIVVS DPYPSASAVA ADLILPTCMW VEKEGAYGNA ERRTQFWRQQ 550
VKGPGQSRSD LWQIVEFSKY FKTEEVWSEE LLAQMPEYRG KTLYEVLYLN 600
GEVNKFQTPT NVPGYINDEA EDFGFYLQKG LFEEYASFGR GHGHDLADFD 650
TYHQVRGLRW PVVDGKETLW RYREGYDPYV KAGEEVSFYG YPDKKAIILG 700
VPYEAPAESP DEEYDLWLCT GRVLEHWHTG TMTRRVPELH KAFPNNLCWM 750
HPTDAKKRGL RHGDKVKLIT RRGEMISHLD TRGRNKCPEG LIYTTFFDAG 800
QLANKLTLDA TDPISGETDY KKCAVKVVKA 830
Length:830
Mass (Da):93,765
Last modified:February 7, 2006 - v2
Checksum:iAF0EDF9F2472B59E
GO

Sequence cautioni

The sequence AAU38888.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016827 Genomic DNA. Translation: AAU38888.1. Different initiation.
RefSeqiYP_089473.1. NC_006300.1.

Genome annotation databases

EnsemblBacteriaiAAU38888; AAU38888; MS2281.
GeneIDi3075593.
KEGGimsu:MS2281.
PATRICi22447767. VBIManSuc86752_2085.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016827 Genomic DNA. Translation: AAU38888.1 . Different initiation.
RefSeqi YP_089473.1. NC_006300.1.

3D structure databases

ProteinModelPortali Q65Q72.
SMRi Q65Q72. Positions 38-829.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 221988.MS2281.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU38888 ; AAU38888 ; MS2281 .
GeneIDi 3075593.
KEGGi msu:MS2281.
PATRICi 22447767. VBIManSuc86752_2085.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci BMAN221988:GHGM-2340-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
    Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
    Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MBEL55E.

Entry informationi

Entry nameiNAPA_MANSM
AccessioniPrimary (citable) accession number: Q65Q72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: May 14, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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