Reviewed, UniProtKB/Swiss-Prot Q65Q72 (NAPA_MANSM)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	MS2281

Organism

Mannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]

Taxonomic identifier

221988 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Mannheimia

Protein attributes

Sequence length	830 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity.
Subcellular location	PeriplasmBy similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Tat-type signal Potential
	Chain	33 – 830	798	Periplasmic nitrate reductase		PRO_0000045991
Sites
	Metal binding	46	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	53	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	81	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65Q72-1 [UniParc]. Last modified February 7, 2006. Version 2. Checksum: AF0EDF9F2472B59E	FASTA	830	93,765
	10 20 30 40 50 60 MELNRRDFMK ANAAVAAAAA AGITIPVKNV HAADDDMGIR WDKAPCRYCG TGCSVLVGTK 70 80 90 100 110 120 DGRVVATQGD PDAEVNRGLN CIKGYFLSKI MYGADRVQTP LLRMKDGKFH KEGDFTPVSW 130 140 150 160 170 180 DQAFTVMADK IKAILKEKKD PNAIGMFSSG QTTIFEGYAK VKLWKAGLRS NTIDPNARHC 190 200 210 220 230 240 MASAAVAFLR TFGMDEPMGC YNDIEKTDAF VLWGSNMAEM HPILWSRISD RRLSSDKVKV 250 260 270 280 290 300 VVMSTFEHRS FELADTPIIF KPHSDLAILN YIANYIIQND KVNWDFVNKH TKFKRGETDI 310 320 330 340 350 360 GYGLRPEHPL EVAAKNRKTA GKMYDSDFEE FKKIVAPYTL EEAHRISGVP KDQLETLAKM 370 380 390 400 410 420 YADPQQNLVS FWTMGFNQHT RGVWVNHMVY NVHLLTGKIS KPGCGPFSLT GQPSACGTAR 430 440 450 460 470 480 EVGTFVHRLP ADMVVTNPKH VEIAENIWKL PKGTISNKPG FPAVQQSRAL KDGKLNFLWQ 490 500 510 520 530 540 LCTNNMQGGP NINEEIFPGW RNPDNLIVVS DPYPSASAVA ADLILPTCMW VEKEGAYGNA 550 560 570 580 590 600 ERRTQFWRQQ VKGPGQSRSD LWQIVEFSKY FKTEEVWSEE LLAQMPEYRG KTLYEVLYLN 610 620 630 640 650 660 GEVNKFQTPT NVPGYINDEA EDFGFYLQKG LFEEYASFGR GHGHDLADFD TYHQVRGLRW 670 680 690 700 710 720 PVVDGKETLW RYREGYDPYV KAGEEVSFYG YPDKKAIILG VPYEAPAESP DEEYDLWLCT 730 740 750 760 770 780 GRVLEHWHTG TMTRRVPELH KAFPNNLCWM HPTDAKKRGL RHGDKVKLIT RRGEMISHLD 790 800 810 820 830 TRGRNKCPEG LIYTTFFDAG QLANKLTLDA TDPISGETDY KKCAVKVVKA

References

[1]

"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens." Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J. Nat. Biotechnol. 22:1275-1281(2004) [PubMed: 15378067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016827 Genomic DNA. Translation: AAU38888.1. Different initiation.
RefSeq	YP_089473.1.
3D structure databases
SMR	Q65Q72. Positions 38-829.
ModBase	Search...
Genome annotation databases
GeneID	3075593.
GenomeReviews	Gene locus MS2281 in contig AE016827_GR.
KEGG	msu:MS2281.
NMPDR	fig|221988.1.peg.2146.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65Q72.
Family and domain databases
HAMAP	MF_01630. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_MANSM

Accession

Primary (citable) accession number: Q65Q72

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	November 25, 2008
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)