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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Mannheimia succiniciproducens (strain MBEL55E)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciBMAN221988:GHGM-2340-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:MS2281
OrganismiMannheimia succiniciproducens (strain MBEL55E)
Taxonomic identifieri221988 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia
ProteomesiUP000000607 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
BLAST
Chaini33 – 830798Periplasmic nitrate reductasePRO_0000045991Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi221988.MS2281.

Structurei

3D structure databases

ProteinModelPortaliQ65Q72.
SMRiQ65Q72. Positions 38-829.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q65Q72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELNRRDFMK ANAAVAAAAA AGITIPVKNV HAADDDMGIR WDKAPCRYCG
60 70 80 90 100
TGCSVLVGTK DGRVVATQGD PDAEVNRGLN CIKGYFLSKI MYGADRVQTP
110 120 130 140 150
LLRMKDGKFH KEGDFTPVSW DQAFTVMADK IKAILKEKKD PNAIGMFSSG
160 170 180 190 200
QTTIFEGYAK VKLWKAGLRS NTIDPNARHC MASAAVAFLR TFGMDEPMGC
210 220 230 240 250
YNDIEKTDAF VLWGSNMAEM HPILWSRISD RRLSSDKVKV VVMSTFEHRS
260 270 280 290 300
FELADTPIIF KPHSDLAILN YIANYIIQND KVNWDFVNKH TKFKRGETDI
310 320 330 340 350
GYGLRPEHPL EVAAKNRKTA GKMYDSDFEE FKKIVAPYTL EEAHRISGVP
360 370 380 390 400
KDQLETLAKM YADPQQNLVS FWTMGFNQHT RGVWVNHMVY NVHLLTGKIS
410 420 430 440 450
KPGCGPFSLT GQPSACGTAR EVGTFVHRLP ADMVVTNPKH VEIAENIWKL
460 470 480 490 500
PKGTISNKPG FPAVQQSRAL KDGKLNFLWQ LCTNNMQGGP NINEEIFPGW
510 520 530 540 550
RNPDNLIVVS DPYPSASAVA ADLILPTCMW VEKEGAYGNA ERRTQFWRQQ
560 570 580 590 600
VKGPGQSRSD LWQIVEFSKY FKTEEVWSEE LLAQMPEYRG KTLYEVLYLN
610 620 630 640 650
GEVNKFQTPT NVPGYINDEA EDFGFYLQKG LFEEYASFGR GHGHDLADFD
660 670 680 690 700
TYHQVRGLRW PVVDGKETLW RYREGYDPYV KAGEEVSFYG YPDKKAIILG
710 720 730 740 750
VPYEAPAESP DEEYDLWLCT GRVLEHWHTG TMTRRVPELH KAFPNNLCWM
760 770 780 790 800
HPTDAKKRGL RHGDKVKLIT RRGEMISHLD TRGRNKCPEG LIYTTFFDAG
810 820 830
QLANKLTLDA TDPISGETDY KKCAVKVVKA
Length:830
Mass (Da):93,765
Last modified:February 7, 2006 - v2
Checksum:iAF0EDF9F2472B59E
GO

Sequence cautioni

The sequence AAU38888.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016827 Genomic DNA. Translation: AAU38888.1. Different initiation.
RefSeqiWP_041640098.1. NC_006300.1.

Genome annotation databases

EnsemblBacteriaiAAU38888; AAU38888; MS2281.
KEGGimsu:MS2281.
PATRICi22447767. VBIManSuc86752_2085.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016827 Genomic DNA. Translation: AAU38888.1. Different initiation.
RefSeqiWP_041640098.1. NC_006300.1.

3D structure databases

ProteinModelPortaliQ65Q72.
SMRiQ65Q72. Positions 38-829.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi221988.MS2281.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU38888; AAU38888; MS2281.
KEGGimsu:MS2281.
PATRICi22447767. VBIManSuc86752_2085.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciBMAN221988:GHGM-2340-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
    Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
    Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MBEL55E.

Entry informationi

Entry nameiNAPA_MANSM
AccessioniPrimary (citable) accession number: Q65Q72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: July 22, 2015
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.