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Q65Q72 (NAPA_MANSM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:MS2281
OrganismMannheimia succiniciproducens (strain MBEL55E) [Complete proteome] [HAMAP]
Taxonomic identifier221988 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeBasfia

Protein attributes

Sequence length830 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence caution

The sequence AAU38888.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal Potential
Chain33 – 830798Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045991

Regions

Domain39 – 95574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding461Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding531Iron-sulfur (4Fe-4S) By similarity
Metal binding811Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65Q72 [UniParc].

Last modified February 7, 2006. Version 2.
Checksum: AF0EDF9F2472B59E

FASTA83093,765
        10         20         30         40         50         60 
MELNRRDFMK ANAAVAAAAA AGITIPVKNV HAADDDMGIR WDKAPCRYCG TGCSVLVGTK 

        70         80         90        100        110        120 
DGRVVATQGD PDAEVNRGLN CIKGYFLSKI MYGADRVQTP LLRMKDGKFH KEGDFTPVSW 

       130        140        150        160        170        180 
DQAFTVMADK IKAILKEKKD PNAIGMFSSG QTTIFEGYAK VKLWKAGLRS NTIDPNARHC 

       190        200        210        220        230        240 
MASAAVAFLR TFGMDEPMGC YNDIEKTDAF VLWGSNMAEM HPILWSRISD RRLSSDKVKV 

       250        260        270        280        290        300 
VVMSTFEHRS FELADTPIIF KPHSDLAILN YIANYIIQND KVNWDFVNKH TKFKRGETDI 

       310        320        330        340        350        360 
GYGLRPEHPL EVAAKNRKTA GKMYDSDFEE FKKIVAPYTL EEAHRISGVP KDQLETLAKM 

       370        380        390        400        410        420 
YADPQQNLVS FWTMGFNQHT RGVWVNHMVY NVHLLTGKIS KPGCGPFSLT GQPSACGTAR 

       430        440        450        460        470        480 
EVGTFVHRLP ADMVVTNPKH VEIAENIWKL PKGTISNKPG FPAVQQSRAL KDGKLNFLWQ 

       490        500        510        520        530        540 
LCTNNMQGGP NINEEIFPGW RNPDNLIVVS DPYPSASAVA ADLILPTCMW VEKEGAYGNA 

       550        560        570        580        590        600 
ERRTQFWRQQ VKGPGQSRSD LWQIVEFSKY FKTEEVWSEE LLAQMPEYRG KTLYEVLYLN 

       610        620        630        640        650        660 
GEVNKFQTPT NVPGYINDEA EDFGFYLQKG LFEEYASFGR GHGHDLADFD TYHQVRGLRW 

       670        680        690        700        710        720 
PVVDGKETLW RYREGYDPYV KAGEEVSFYG YPDKKAIILG VPYEAPAESP DEEYDLWLCT 

       730        740        750        760        770        780 
GRVLEHWHTG TMTRRVPELH KAFPNNLCWM HPTDAKKRGL RHGDKVKLIT RRGEMISHLD 

       790        800        810        820        830 
TRGRNKCPEG LIYTTFFDAG QLANKLTLDA TDPISGETDY KKCAVKVVKA 

« Hide

References

[1]"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens."
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.
Nat. Biotechnol. 22:1275-1281(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MBEL55E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016827 Genomic DNA. Translation: AAU38888.1. Different initiation.
RefSeqYP_089473.1. NC_006300.1.

3D structure databases

ProteinModelPortalQ65Q72.
SMRQ65Q72. Positions 38-829.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING221988.MS2281.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU38888; AAU38888; MS2281.
GeneID3075593.
KEGGmsu:MS2281.
PATRIC22447767. VBIManSuc86752_2085.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycBMAN221988:GHGM-2340-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_MANSM
AccessionPrimary (citable) accession number: Q65Q72
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: February 19, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families