Reviewed, UniProtKB/Swiss-Prot Q65PH5 (ISPE_BACLD)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	BLi00059, BL00525

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235064
Regions
	Nucleotide binding	94 – 104	11	ATP Potential
Sites
	Active site	10	1	By similarity
	Active site	136	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65PH5-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 4323BD08948F4B38	FASTA	289	31,943
	10 20 30 40 50 60 MRILEKAPAK INLSLDVRHK RPDGYHEVEM VMTTIDLADR VELTELAEDK VTVSSHNRFV 70 80 90 100 110 120 PDDQRNLAYQ AAMLMKERYG IKKGVSIFIT KVIPVAAGLA GGSSDAAAVF RGLNRLWDLK 130 140 150 160 170 180 LSMNELAELG AEIGSDVSFC VHGGTALATG RGEKIRHIET PPHCWVVLAK PTIGVSTAEV 190 200 210 220 230 240 YKQLKVDEIE HPDVQGMIAA IEEKNFQKMC DKLGNVLESV TLNMHPEVAM IKNQMKRFGA 250 260 270 280 DAVLMSGSGP TVFGLVQYES KVQRIYNGLR GFCDQVYAVR MIGEQNELD

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU39039.1. CP000002 Genomic DNA. Translation: AAU21694.2.
RefSeq	YP_077332.2. YP_089732.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q65PH5.
Genome annotation databases
GeneID	3027307. 3101513.
GenomeReviews	Gene locus BLi00059 in contig AE017333_GR. Gene locus BL00525 in contig CP000002_GR.
KEGG	bld:BLi00059. bli:BL00525.
NMPDR	fig|279010.5.peg.1341.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65PH5.
Enzyme and pathway databases
BioCyc	BLIC279010:BL00525-MON.
Family and domain databases
HAMAP	MF_00061. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_BACLD

Accession

Primary (citable) accession number: Q65PH5
Secondary accession number(s): Q62ZW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	October 25, 2004
Last modified:	September 22, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)