Q65NN6 (NADE_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BLi00370, BL01706

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077537
Regions
	Nucleotide binding	45 – 52	8	ATP By similarity
Sites
	Active site	47	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65NN6 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 37C8BA51D6140A4C	FASTA	272	29,997
	10 20 30 40 50 60 MTLQEKIMQE LNVKPSIEPK QEIEKRVGFL KSYLKKTGAK GFVLGISGGQ DSTLAGRLAQ 70 80 90 100 110 120 LAVEELREEG IQAEFIAVRL PYGVQQDEDD AQLALKFIQP DKSFAFDIAS TVGSFAAQYQ 130 140 150 160 170 180 SVTGEALADF HKGNVKARVR MITQYAIGGQ NQLLVIGTDH AAEAVTGFFT KYGDGGADLL 190 200 210 220 230 240 PLTGLTKRQG RSLLEELGAP ERLYTKSPTA DLLDEKPQQS DETELGLTYD NIDDYLEGKA 250 260 270 VSSEVAEAIE KRYKASEHKR QVPASMFDDW WK

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000002 Genomic DNA. Translation: AAU21974.1. AE017333 Genomic DNA. Translation: AAU39328.1.
RefSeq	YP_077612.1. NC_006270.3. YP_090021.1. NC_006322.1.
3D structure databases
HSSP	HSSP built from PDB template 1KQP based on UniProtKB P08164.
ProteinModelPortal	Q65NN6.
SMR	Q65NN6. Positions 2-272.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65NN6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000055073; EBBACP00000053604; EBBACG00000055064. EBBACT00000062619; EBBACP00000061052; EBBACG00000062610.
GeneID	3028255. 3099555.
GenomeReviews	Gene locus BLi00370 in contig AE017333_GR. Gene locus BL01706 in contig CP000002_GR.
KEGG	bld:BLi00370. bli:BL01706.
NMPDR	fig|279010.5.peg.2223.
PATRIC	18946235. VBIBacLic203714_0356.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
GeneTree	EBGT00050000001308.
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI00370-MONOMER. BLIC279010:BL01706-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BACLD

Accession

Primary (citable) accession number: Q65NN6
Secondary accession number(s): Q62Z34

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program