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Protein

1-pyrroline-5-carboxylate dehydrogenase

Gene

rocA

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH.UniRule annotation

Pathwayi: L-proline degradation into L-glutamate

This protein is involved in step 2 of the subpathway that synthesizes L-glutamate from L-proline.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 1-pyrroline-5-carboxylate dehydrogenase (rocA)
This subpathway is part of the pathway L-proline degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from L-proline, the pathway L-proline degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei287 – 2871UniRule annotation
Active sitei321 – 3211UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-366-MONOMER.
UniPathwayiUPA00261; UER00374.

Names & Taxonomyi

Protein namesi
Recommended name:
1-pyrroline-5-carboxylate dehydrogenaseUniRule annotation (EC:1.2.1.88UniRule annotation)
Short name:
P5C dehydrogenaseUniRule annotation
Alternative name(s):
L-glutamate gamma-semialdehyde dehydrogenaseUniRule annotation
Gene namesi
Name:rocAUniRule annotation
Ordered Locus Names:BLi00374, BL01710
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000606 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5165161-pyrroline-5-carboxylate dehydrogenasePRO_0000056510Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi279010.BLi00374.

Structurei

Secondary structure

1
516
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 3116Combined sources
Turni32 – 354Combined sources
Beta strandi36 – 383Combined sources
Beta strandi40 – 423Combined sources
Beta strandi45 – 473Combined sources
Beta strandi50 – 578Combined sources
Beta strandi60 – 6910Combined sources
Helixi73 – 9018Combined sources
Helixi96 – 11116Combined sources
Helixi113 – 12412Combined sources
Helixi128 – 15225Combined sources
Beta strandi164 – 1729Combined sources
Beta strandi175 – 1795Combined sources
Turni183 – 1864Combined sources
Helixi187 – 19812Combined sources
Beta strandi202 – 2054Combined sources
Helixi213 – 22311Combined sources
Turni224 – 2263Combined sources
Beta strandi231 – 2344Combined sources
Turni239 – 2413Combined sources
Helixi242 – 2487Combined sources
Beta strandi252 – 2598Combined sources
Helixi261 – 27212Combined sources
Beta strandi283 – 2875Combined sources
Beta strandi293 – 2964Combined sources
Beta strandi298 – 3003Combined sources
Helixi302 – 31413Combined sources
Helixi315 – 3184Combined sources
Beta strandi326 – 3305Combined sources
Turni331 – 3333Combined sources
Helixi334 – 34512Combined sources
Beta strandi348 – 3514Combined sources
Beta strandi358 – 3603Combined sources
Helixi366 – 38217Combined sources
Beta strandi383 – 3875Combined sources
Beta strandi394 – 3963Combined sources
Beta strandi402 – 4065Combined sources
Helixi412 – 4143Combined sources
Beta strandi420 – 43011Combined sources
Helixi431 – 4388Combined sources
Beta strandi441 – 45010Combined sources
Helixi454 – 46310Combined sources
Beta strandi467 – 4737Combined sources
Turni480 – 4823Combined sources
Beta strandi499 – 5024Combined sources
Helixi503 – 5064Combined sources
Beta strandi507 – 5159Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RJLX-ray2.20A/B/C/D/E/F/G/H1-516[»]
ProteinModelPortaliQ65NN2.
SMRiQ65NN2. Positions 3-516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family. RocA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C26. Bacteria.
COG1012. LUCA.
HOGENOMiHOG000271511.
KOiK00294.
OMAiWLKRTIL.
OrthoDBiEOG6BS8QW.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00733. RocA.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR005932. RocA.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01237. D1pyr5carbox2. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65NN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPYKHEPF TNFGIEENRK AFEKALETVN NEWLGQSYPL VIDGERYETE
60 70 80 90 100
NKIVSINPAN KEEVVGTVSK ATQDHAEKAI QAAAKAFETW RYTDPEERAA
110 120 130 140 150
VLFRAVAKVR RKKHEFSALL VKEAGKPWNE ADADTAEAID FMEYYARQMI
160 170 180 190 200
ELAKGKPVNS REGERNQYVY TPTGVTVVIP PWNFLFAIMA GTTVAPIVTG
210 220 230 240 250
NTVVLKPASA APVIAAKFVE VLEESGLPKG VVNFVPGSGA EVGDYLVDHP
260 270 280 290 300
KTSIITFTGS REVGTRIFER AAKVQPGQTH LKQVIAEMGG KDTVVVDEDC
310 320 330 340 350
DIELAAQSIF TSAFGFAGQK CSAGSRAVVH EKVYDEVLKR VIEITESKKV
360 370 380 390 400
GEPDSADVYM GPVIDQASFN KIMDYIEIGK EEGRLVSGGK GDDSKGYFIE
410 420 430 440 450
PTIFADLDPK ARLMQEEIFG PVVAFSKVSS FDEALEVANN TEYGLTGAVI
460 470 480 490 500
TKNRDHINRA KQEFHVGNLY FNRNCTGAIV GYHPFGGFKM SGTDSKAGGP
510
DYLALHMQAK TISEMF
Length:516
Mass (Da):56,655
Last modified:October 25, 2004 - v1
Checksum:i9BB008CC70ABBFDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU39332.1.
CP000002 Genomic DNA. Translation: AAU21978.1.
RefSeqiWP_011197529.1. NC_006322.1.

Genome annotation databases

EnsemblBacteriaiAAU21978; AAU21978; BL01710.
AAU39332; AAU39332; BLi00374.
GeneIDi3027308.
KEGGibld:BLi00374.
bli:BL01710.
PATRICi18946245. VBIBacLic203714_0361.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU39332.1.
CP000002 Genomic DNA. Translation: AAU21978.1.
RefSeqiWP_011197529.1. NC_006322.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RJLX-ray2.20A/B/C/D/E/F/G/H1-516[»]
ProteinModelPortaliQ65NN2.
SMRiQ65NN2. Positions 3-516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279010.BLi00374.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU21978; AAU21978; BL01710.
AAU39332; AAU39332; BLi00374.
GeneIDi3027308.
KEGGibld:BLi00374.
bli:BL01710.
PATRICi18946245. VBIBacLic203714_0361.

Phylogenomic databases

eggNOGiENOG4105C26. Bacteria.
COG1012. LUCA.
HOGENOMiHOG000271511.
KOiK00294.
OMAiWLKRTIL.
OrthoDBiEOG6BS8QW.

Enzyme and pathway databases

UniPathwayiUPA00261; UER00374.
BioCyciBLIC279010:GJ2P-366-MONOMER.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00733. RocA.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR005932. RocA.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01237. D1pyr5carbox2. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46.

Entry informationi

Entry nameiROCA_BACLD
AccessioniPrimary (citable) accession number: Q65NN2
Secondary accession number(s): Q62Z30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 25, 2004
Last modified: January 20, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.