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Protein

1-pyrroline-5-carboxylate dehydrogenase

Gene

rocA

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH.UniRule annotation

Pathwayi: L-proline degradation into L-glutamate

This protein is involved in step 2 of the subpathway that synthesizes L-glutamate from L-proline.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 1-pyrroline-5-carboxylate dehydrogenase (rocA)
This subpathway is part of the pathway L-proline degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from L-proline, the pathway L-proline degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei287UniRule annotation1
Active sitei321UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00261; UER00374.

Names & Taxonomyi

Protein namesi
Recommended name:
1-pyrroline-5-carboxylate dehydrogenaseUniRule annotation (EC:1.2.1.88UniRule annotation)
Short name:
P5C dehydrogenaseUniRule annotation
Alternative name(s):
L-glutamate gamma-semialdehyde dehydrogenaseUniRule annotation
Gene namesi
Name:rocAUniRule annotation
Ordered Locus Names:BLi00374, BL01710
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000606 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000565101 – 5161-pyrroline-5-carboxylate dehydrogenaseAdd BLAST516

Interactioni

Protein-protein interaction databases

STRINGi279010.BLi00374.

Structurei

Secondary structure

1516
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 31Combined sources16
Turni32 – 35Combined sources4
Beta strandi36 – 38Combined sources3
Beta strandi40 – 42Combined sources3
Beta strandi45 – 47Combined sources3
Beta strandi50 – 57Combined sources8
Beta strandi60 – 69Combined sources10
Helixi73 – 90Combined sources18
Helixi96 – 111Combined sources16
Helixi113 – 124Combined sources12
Helixi128 – 152Combined sources25
Beta strandi164 – 172Combined sources9
Beta strandi175 – 179Combined sources5
Turni183 – 186Combined sources4
Helixi187 – 198Combined sources12
Beta strandi202 – 205Combined sources4
Helixi213 – 223Combined sources11
Turni224 – 226Combined sources3
Beta strandi231 – 234Combined sources4
Turni239 – 241Combined sources3
Helixi242 – 248Combined sources7
Beta strandi252 – 259Combined sources8
Helixi261 – 272Combined sources12
Beta strandi283 – 287Combined sources5
Beta strandi293 – 296Combined sources4
Beta strandi298 – 300Combined sources3
Helixi302 – 314Combined sources13
Helixi315 – 318Combined sources4
Beta strandi326 – 330Combined sources5
Turni331 – 333Combined sources3
Helixi334 – 345Combined sources12
Beta strandi348 – 351Combined sources4
Beta strandi358 – 360Combined sources3
Helixi366 – 382Combined sources17
Beta strandi383 – 387Combined sources5
Beta strandi394 – 396Combined sources3
Beta strandi402 – 406Combined sources5
Helixi412 – 414Combined sources3
Beta strandi420 – 430Combined sources11
Helixi431 – 438Combined sources8
Beta strandi441 – 450Combined sources10
Helixi454 – 463Combined sources10
Beta strandi467 – 473Combined sources7
Turni480 – 482Combined sources3
Beta strandi499 – 502Combined sources4
Helixi503 – 506Combined sources4
Beta strandi507 – 515Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RJLX-ray2.20A/B/C/D/E/F/G/H1-516[»]
ProteinModelPortaliQ65NN2.
SMRiQ65NN2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family. RocA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C26. Bacteria.
COG1012. LUCA.
HOGENOMiHOG000271511.
KOiK00294.
OMAiPVVGFTK.

Family and domain databases

CDDicd07124. ALDH_PutA-P5CDH-RocA. 1 hit.
Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00733. RocA. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR005932. RocA.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01237. D1pyr5carbox2. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65NN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPYKHEPF TNFGIEENRK AFEKALETVN NEWLGQSYPL VIDGERYETE
60 70 80 90 100
NKIVSINPAN KEEVVGTVSK ATQDHAEKAI QAAAKAFETW RYTDPEERAA
110 120 130 140 150
VLFRAVAKVR RKKHEFSALL VKEAGKPWNE ADADTAEAID FMEYYARQMI
160 170 180 190 200
ELAKGKPVNS REGERNQYVY TPTGVTVVIP PWNFLFAIMA GTTVAPIVTG
210 220 230 240 250
NTVVLKPASA APVIAAKFVE VLEESGLPKG VVNFVPGSGA EVGDYLVDHP
260 270 280 290 300
KTSIITFTGS REVGTRIFER AAKVQPGQTH LKQVIAEMGG KDTVVVDEDC
310 320 330 340 350
DIELAAQSIF TSAFGFAGQK CSAGSRAVVH EKVYDEVLKR VIEITESKKV
360 370 380 390 400
GEPDSADVYM GPVIDQASFN KIMDYIEIGK EEGRLVSGGK GDDSKGYFIE
410 420 430 440 450
PTIFADLDPK ARLMQEEIFG PVVAFSKVSS FDEALEVANN TEYGLTGAVI
460 470 480 490 500
TKNRDHINRA KQEFHVGNLY FNRNCTGAIV GYHPFGGFKM SGTDSKAGGP
510
DYLALHMQAK TISEMF
Length:516
Mass (Da):56,655
Last modified:October 25, 2004 - v1
Checksum:i9BB008CC70ABBFDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU39332.1.
CP000002 Genomic DNA. Translation: AAU21978.1.
RefSeqiWP_011197529.1. NC_006322.1.

Genome annotation databases

EnsemblBacteriaiAAU21978; AAU21978; BL01710.
AAU39332; AAU39332; BLi00374.
GeneIDi3027308.
KEGGibld:BLi00374.
bli:BL01710.
PATRICi18946245. VBIBacLic203714_0361.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU39332.1.
CP000002 Genomic DNA. Translation: AAU21978.1.
RefSeqiWP_011197529.1. NC_006322.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RJLX-ray2.20A/B/C/D/E/F/G/H1-516[»]
ProteinModelPortaliQ65NN2.
SMRiQ65NN2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279010.BLi00374.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU21978; AAU21978; BL01710.
AAU39332; AAU39332; BLi00374.
GeneIDi3027308.
KEGGibld:BLi00374.
bli:BL01710.
PATRICi18946245. VBIBacLic203714_0361.

Phylogenomic databases

eggNOGiENOG4105C26. Bacteria.
COG1012. LUCA.
HOGENOMiHOG000271511.
KOiK00294.
OMAiPVVGFTK.

Enzyme and pathway databases

UniPathwayiUPA00261; UER00374.

Family and domain databases

CDDicd07124. ALDH_PutA-P5CDH-RocA. 1 hit.
Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00733. RocA. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR005932. RocA.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01237. D1pyr5carbox2. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiROCA_BACLD
AccessioniPrimary (citable) accession number: Q65NN2
Secondary accession number(s): Q62Z30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 25, 2004
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.