Reviewed, UniProtKB/Swiss-Prot Q65NN2 (ROCA_BACLD)

Names and origin

Protein names

Recommended name:
    1-pyrroline-5-carboxylate dehydrogenase
      Short name=P5C dehydrogenase
    EC=1.5.1.12

Gene names

Name:	rocA
Ordered Locus Names:	BLi00374, BL01710

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 516	516	1-pyrroline-5-carboxylate dehydrogenase HAMAP MF_00733		PRO_0000056510
Sites
	Active site	287	1	By similarity
	Active site	321	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65NN2-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 9BB008CC70ABBFDB	FASTA	516	56,655
	10 20 30 40 50 60 MTTPYKHEPF TNFGIEENRK AFEKALETVN NEWLGQSYPL VIDGERYETE NKIVSINPAN 70 80 90 100 110 120 KEEVVGTVSK ATQDHAEKAI QAAAKAFETW RYTDPEERAA VLFRAVAKVR RKKHEFSALL 130 140 150 160 170 180 VKEAGKPWNE ADADTAEAID FMEYYARQMI ELAKGKPVNS REGERNQYVY TPTGVTVVIP 190 200 210 220 230 240 PWNFLFAIMA GTTVAPIVTG NTVVLKPASA APVIAAKFVE VLEESGLPKG VVNFVPGSGA 250 260 270 280 290 300 EVGDYLVDHP KTSIITFTGS REVGTRIFER AAKVQPGQTH LKQVIAEMGG KDTVVVDEDC 310 320 330 340 350 360 DIELAAQSIF TSAFGFAGQK CSAGSRAVVH EKVYDEVLKR VIEITESKKV GEPDSADVYM 370 380 390 400 410 420 GPVIDQASFN KIMDYIEIGK EEGRLVSGGK GDDSKGYFIE PTIFADLDPK ARLMQEEIFG 430 440 450 460 470 480 PVVAFSKVSS FDEALEVANN TEYGLTGAVI TKNRDHINRA KQEFHVGNLY FNRNCTGAIV 490 500 510 GYHPFGGFKM SGTDSKAGGP DYLALHMQAK TISEMF

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU39332.1. CP000002 Genomic DNA. Translation: AAU21978.1.
RefSeq	YP_077616.1. YP_090025.1.
3D structure databases
SMR	Q65NN2. Positions 3-516.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65NN2.
Genome annotation databases
GeneID	3027308. 3097821.
GenomeReviews	Gene locus BLi00374 in contig AE017333_GR. Gene locus BL01710 in contig CP000002_GR.
KEGG	bld:BLi00374. bli:BL01710.
NMPDR	fig|279010.5.peg.2227.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HBG752218.
OMA	TPYKHEP.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI00374-MONOMER. BLIC279010:BL01710-MONOMER.
Family and domain databases
HAMAP	MF_00733. RocA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR005932. d-1-pyrroline-5-COlate_DH-2. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ROCA_BACLD

Accession

Primary (citable) accession number: Q65NN2
Secondary accession number(s): Q62Z30

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	October 25, 2004
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)