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Q65N90 (TOP3_BACLD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 3

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase III
Gene names
Name:topB
Ordered Locus Names:BLi00518, BL02823
OrganismBacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]
Taxonomic identifier279010 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length727 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00953

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00953

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00953

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence caution

The sequence AAU22114.2 differs from that shown. Reason: Erroneous initiation.

The sequence AAU39474.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 727727DNA topoisomerase 3 HAMAP-Rule MF_00953
PRO_0000286364

Regions

Domain3 – 136134Toprim
Region187 – 1926Interaction with DNA By similarity

Sites

Active site3101O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium; catalytic By similarity
Metal binding1051Magnesium; catalytic By similarity
Site611Interaction with DNA By similarity
Site1681Interaction with DNA By similarity
Site1761Interaction with DNA By similarity
Site3121Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65N90 [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: 6649802B7503A542

FASTA72781,504
        10         20         30         40         50         60 
MSKTVVLAEK PSVGRDLARV LKCHKKGNGY LEGDRYIVTW ALGHLVTLAD PEGYGKEYQS 

        70         80         90        100        110        120 
WRLEDLPIIP EPLKLVVIKK TGKQFQAVKS QLIRKDVKDI VIATDAGREG ELVARWIIEK 

       130        140        150        160        170        180 
AKVTKPLKRL WISSVTDKAI KDGFKNLKNG KDFENLYHSA VARAEADWIV GINATRALTT 

       190        200        210        220        230        240 
KFNAQLSCGR VQTPTLAMIA KREEDIKNFK PVPYFGLRAA VDGMTLTWQD KKTSQTRTFN 

       250        260        270        280        290        300 
ASVTSQLAGA LQGKPAVIVD LKKTAKKSFA PGLYDLTELQ RDAHKRFGFS AKETLSVLQK 

       310        320        330        340        350        360 
LYEQHKLVTY PRTDSRFLSN DIVPTLKDRL EGMQVKPYAQ HVARILKRGV KANKSFVNDA 

       370        380        390        400        410        420 
KVSDHHAIIP TEEPLALGAL SEKERKLYDL IAKRFLAVLM PPFEYEETKV FAEIGGETFT 

       430        440        450        460        470        480 
AKGKTVQSQG WKAVYDFADE DDDEEEKDQT LPKLAKGDTL SVRSLTETKG ETKPPARFNE 

       490        500        510        520        530        540 
GTLLSAMENP AAFMQGEEKN LVKTLGETGG LGTVATRADI IEKLFNTFLI EKKGKDIYIT 

       550        560        570        580        590        600 
SKGKQLLELV PSDLKSPALT AEWEQKLSGI AKGKLKSSAF IKEMKEYAKQ TIREIKSSNQ 

       610        620        630        640        650        660 
KFKHDNITGT HCPDCGKLML KVNGKRGTML VCQDRECGHR KTVAKQTNAR CPVCHKRMEL 

       670        680        690        700        710        720 
RGHGEGQTFA CVCGHREKLS VFEKRRAKDK NSKASKRDVH SYMKKQNKDE PINNALAEQL 


KKLKLDQ 

« Hide

References

[1]"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.
[2]"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species."
Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. expand/collapse author list , Galleron N., Ehrlich S.D., Berka R.M.
Genome Biol. 5:R77.1-R77.12(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017333 Genomic DNA. Translation: AAU39474.1. Different initiation.
CP000002 Genomic DNA. Translation: AAU22114.2. Different initiation.
RefSeqYP_006711945.1. NC_006322.1.
YP_077752.2. NC_006270.3.

3D structure databases

ProteinModelPortalQ65N90.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279010.BL02823.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU22114; AAU22114; BL02823.
AAU39474; AAU39474; BLi00518.
GeneID3030214.
3101651.
KEGGbld:BLi00518.
bli:BL02823.
PATRIC18946539. VBIBacLic203714_0508.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000086847.
KOK03169.
OMATKHDEYF.
OrthoDBEOG60CWM6.
ProtClustDBPRK07726.

Enzyme and pathway databases

BioCycBLIC279010:GJ2P-506-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00953. Topoisom_3_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01056. topB. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP3_BACLD
AccessionPrimary (citable) accession number: Q65N90
Secondary accession number(s): Q62YP4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: February 19, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families