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Q65N90

- TOP3_BACLD

UniProt

Q65N90 - TOP3_BACLD

Protein

DNA topoisomerase 3

Gene

topB

Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Magnesium; catalyticUniRule annotation
    Sitei61 – 611Interaction with DNAUniRule annotation
    Metal bindingi105 – 1051Magnesium; catalyticUniRule annotation
    Sitei168 – 1681Interaction with DNAUniRule annotation
    Sitei176 – 1761Interaction with DNAUniRule annotation
    Active sitei310 – 3101O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei312 – 3121Interaction with DNAUniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBLIC279010:GJ2P-506-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IIIUniRule annotation
    Gene namesi
    Name:topBUniRule annotation
    Ordered Locus Names:BLi00518, BL02823
    OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
    Taxonomic identifieri279010 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 727727DNA topoisomerase 3PRO_0000286364Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi279010.BL02823.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65N90.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 136134ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni187 – 1926Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0551.
    HOGENOMiHOG000086847.
    KOiK03169.
    OMAiTKHDEYF.
    OrthoDBiEOG60CWM6.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00953. Topoisom_3_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005738. TopoIII.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01056. topB. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q65N90-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKTVVLAEK PSVGRDLARV LKCHKKGNGY LEGDRYIVTW ALGHLVTLAD    50
    PEGYGKEYQS WRLEDLPIIP EPLKLVVIKK TGKQFQAVKS QLIRKDVKDI 100
    VIATDAGREG ELVARWIIEK AKVTKPLKRL WISSVTDKAI KDGFKNLKNG 150
    KDFENLYHSA VARAEADWIV GINATRALTT KFNAQLSCGR VQTPTLAMIA 200
    KREEDIKNFK PVPYFGLRAA VDGMTLTWQD KKTSQTRTFN ASVTSQLAGA 250
    LQGKPAVIVD LKKTAKKSFA PGLYDLTELQ RDAHKRFGFS AKETLSVLQK 300
    LYEQHKLVTY PRTDSRFLSN DIVPTLKDRL EGMQVKPYAQ HVARILKRGV 350
    KANKSFVNDA KVSDHHAIIP TEEPLALGAL SEKERKLYDL IAKRFLAVLM 400
    PPFEYEETKV FAEIGGETFT AKGKTVQSQG WKAVYDFADE DDDEEEKDQT 450
    LPKLAKGDTL SVRSLTETKG ETKPPARFNE GTLLSAMENP AAFMQGEEKN 500
    LVKTLGETGG LGTVATRADI IEKLFNTFLI EKKGKDIYIT SKGKQLLELV 550
    PSDLKSPALT AEWEQKLSGI AKGKLKSSAF IKEMKEYAKQ TIREIKSSNQ 600
    KFKHDNITGT HCPDCGKLML KVNGKRGTML VCQDRECGHR KTVAKQTNAR 650
    CPVCHKRMEL RGHGEGQTFA CVCGHREKLS VFEKRRAKDK NSKASKRDVH 700
    SYMKKQNKDE PINNALAEQL KKLKLDQ 727
    Length:727
    Mass (Da):81,504
    Last modified:May 1, 2007 - v2
    Checksum:i6649802B7503A542
    GO

    Sequence cautioni

    The sequence AAU22114.2 differs from that shown. Reason: Erroneous initiation.
    The sequence AAU39474.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017333 Genomic DNA. Translation: AAU39474.1. Different initiation.
    CP000002 Genomic DNA. Translation: AAU22114.2. Different initiation.
    RefSeqiYP_006711945.1. NC_006322.1.
    YP_077752.2. NC_006270.3.

    Genome annotation databases

    EnsemblBacteriaiAAU22114; AAU22114; BL02823.
    AAU39474; AAU39474; BLi00518.
    GeneIDi3030214.
    3101651.
    KEGGibld:BLi00518.
    bli:BL02823.
    PATRICi18946539. VBIBacLic203714_0508.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017333 Genomic DNA. Translation: AAU39474.1 . Different initiation.
    CP000002 Genomic DNA. Translation: AAU22114.2 . Different initiation.
    RefSeqi YP_006711945.1. NC_006322.1.
    YP_077752.2. NC_006270.3.

    3D structure databases

    ProteinModelPortali Q65N90.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 279010.BL02823.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU22114 ; AAU22114 ; BL02823 .
    AAU39474 ; AAU39474 ; BLi00518 .
    GeneIDi 3030214.
    3101651.
    KEGGi bld:BLi00518.
    bli:BL02823.
    PATRICi 18946539. VBIBacLic203714_0508.

    Phylogenomic databases

    eggNOGi COG0551.
    HOGENOMi HOG000086847.
    KOi K03169.
    OMAi TKHDEYF.
    OrthoDBi EOG60CWM6.

    Enzyme and pathway databases

    BioCyci BLIC279010:GJ2P-506-MONOMER.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00953. Topoisom_3_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005738. TopoIII.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01056. topB. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
      Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
      J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 13 / ATCC 14580.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 13 / ATCC 14580.

    Entry informationi

    Entry nameiTOP3_BACLD
    AccessioniPrimary (citable) accession number: Q65N90
    Secondary accession number(s): Q62YP4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3