Q65MW0 (SYY2_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase 2
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase 2
Short name=TyrRS 2

Gene names

Name:	tyrS2
Ordered Locus Names:	BLi00666, BL00484

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02007
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity. HAMAP MF_02007
Subcellular location	Cytoplasm By similarity HAMAP MF_02007.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Tyrosine--tRNA ligase 2 HAMAP MF_02007		PRO_0000236694
Regions
	Domain	354 – 415	62	S4 RNA-binding
	Motif	59 – 68	10	"HIGH" region HAMAP MF_02007
	Motif	243 – 247	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	246	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65MW0 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 70CEA3B806D32AF7	FASTA	417	47,192
	10 20 30 40 50 60 MQQQQFDQLT TAEKREVERQ LHLYMDGAEE VIPPEELRAK LAKSILTGEP LKIKLGLDPS 70 80 90 100 110 120 APDVHLGHTV VLNKLRQFQE NGHIVQLLIG DFTGKIGDPT GKSAARKQLT DEEVRRNAET 130 140 150 160 170 180 YFQQFGKVLD QNKVELHYNS KWLKRLTLED VIELAGKITV ARLMERDDFE ERIAMQKPIS 190 200 210 220 230 240 LHEFFYPLMQ GYDSVVLDSD IELGGTDQHF NVLMGRHFQE KYGKEKQVVI LMPLLEGLDG 250 260 270 280 290 300 IEKMSKSKNN YIGIDEDPHE MFGKTMSLPD SLMKKYIHLA TDLEAEEKKR LVKDLETGAV 310 320 330 340 350 360 HPRDAKMLLG KTIVNMYHGS KAAEAAEQQF KKVFQQNSAP DNITAVHWQG GGTVPVTDLL 370 380 390 400 410 VTLELLPSKS EARRMIQNGG VKINGAKAES IHADIALEHG MIIQVGKRKF VKLQMHQ

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU39604.1. CP000002 Genomic DNA. Translation: AAU22255.1.
RefSeq	YP_077893.1. NC_006270.3. YP_090297.1. NC_006322.1.
3D structure databases
ProteinModelPortal	Q65MW0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65MW0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000054858; EBBACP00000053389; EBBACG00000054849. EBBACT00000059664; EBBACP00000058097; EBBACG00000059655.
GeneID	3031036. 3100653.
GenomeReviews	Gene locus BLi00666 in contig AE017333_GR. Gene locus BL00484 in contig CP000002_GR.
KEGG	bld:BLi00666. bli:BL00484.
NMPDR	fig|279010.5.peg.925.
PATRIC	18946853. VBIBacLic203714_0651.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0162.
GeneTree	EBGT00050000000599.
HOGENOM	HBG288125.
OMA	YVVQVGK.
PhylomeDB	Q65MW0.
ProtClustDB	PRK13354.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI00666-MONOMER. BLIC279010:BL00484-MONOMER.
Family and domain databases
HAMAP	MF_02007. Tyr_tRNA_synth_type2. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024108. Tyr-tRNA-synth_bac_2. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY2_BACLD

Accession

Primary (citable) accession number: Q65MW0
Secondary accession number(s): Q62YA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program